Biochemical effects of 17β-estradiol on UMR106 cells
The effect of 17β-estradiol (E) on an osteoblast-like cell line, UMR106, was studied in vitro. The concentrations of transferrin and seven enzymes (gamma glutamyl transferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, alanine aminotransferase and aspettate amino...
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| Veröffentlicht in: | Bone and mineral 1989-04, Vol.6 (1), p.55-63 |
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| creator | Bankson, D.D. Rifai, N. Williams, M.E. Silverman, L.M. Gray, T.K. |
| description | The effect of 17β-estradiol (E) on an osteoblast-like cell line, UMR106, was studied in vitro. The concentrations of transferrin and seven enzymes (gamma glutamyl transferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, alanine aminotransferase and aspettate aminotransferase) were measured in these cells after incubation in culture medium containing either E or the vehicle. E treatment increased five of the seven enzymes and increased the transferrin concentration in the UMR106 cells white simultaneously reducing the proliferation rates, 4-Hydroxytamoxifen, an estrogen antagonist, produced a mild estrogen agonist action on growth rates and enzyme concentrations in the UMR106 cells, When E was present simultaneously, the agonist properties of 4-hydroxytamoxifen were enhanced. These studies show that E enhanced activity of five enzymes and the transferrin content of UMR106 cells after a 2-day incubation. 4-Hydroxytamoxifen enhanced the E effect, illustrating that estrogen antagonists may manifest agonist or antagonist properties depending on the model. These results extend our previous observations showing a direct effect of E in vitro on osteoblast-like cells. |
| doi_str_mv | 10.1016/0169-6009(89)90023-8 |
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The concentrations of transferrin and seven enzymes (gamma glutamyl transferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, alanine aminotransferase and aspettate aminotransferase) were measured in these cells after incubation in culture medium containing either E or the vehicle. E treatment increased five of the seven enzymes and increased the transferrin concentration in the UMR106 cells white simultaneously reducing the proliferation rates, 4-Hydroxytamoxifen, an estrogen antagonist, produced a mild estrogen agonist action on growth rates and enzyme concentrations in the UMR106 cells, When E was present simultaneously, the agonist properties of 4-hydroxytamoxifen were enhanced. These studies show that E enhanced activity of five enzymes and the transferrin content of UMR106 cells after a 2-day incubation. 4-Hydroxytamoxifen enhanced the E effect, illustrating that estrogen antagonists may manifest agonist or antagonist properties depending on the model. These results extend our previous observations showing a direct effect of E in vitro on osteoblast-like cells.</description><identifier>ISSN: 0169-6009</identifier><identifier>DOI: 10.1016/0169-6009(89)90023-8</identifier><identifier>PMID: 2568866</identifier><identifier>CODEN: BOMIET</identifier><language>eng</language><publisher>London: Elsevier Inc</publisher><subject>4-Hydroxytamoxifen ; Acid Phosphatase - metabolism ; Alanine Transaminase - metabolism ; Alkaline Phosphatase - metabolism ; Animals ; Aspartate Aminotransferases - metabolism ; Biological and medical sciences ; Cell Differentiation - drug effects ; Cell Line ; Cells, Cultured ; Creatine Kinase - metabolism ; Enzyme ; Estradiol - pharmacology ; Estrogen ; Estrogen Antagonists - metabolism ; Estrogen Antagonists - pharmacology ; Estrogens - pharmacology ; Fundamental and applied biological sciences. Psychology ; gamma-Glutamyltransferase - metabolism ; Hormone metabolism and regulation ; L-Lactate Dehydrogenase - metabolism ; Mammalian female genital system ; Osteoblastic cell ; Osteoblasts - analysis ; Osteoblasts - drug effects ; Osteoblasts - enzymology ; Rats ; Receptors, Estrogen - drug effects ; Receptors, Estrogen - metabolism ; Tamoxifen - analogs & derivatives ; Tamoxifen - metabolism ; Tamoxifen - pharmacology ; Transferrin ; Transferrin - analysis ; Vertebrates: reproduction</subject><ispartof>Bone and mineral, 1989-04, Vol.6 (1), p.55-63</ispartof><rights>1989 Elsevier Science Publishers B.V. (Biomedical Division)</rights><rights>1991 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-f24026fe1e1e6d91aea893829095832a54465994be92d2d824bcad1182fabb03</citedby><cites>FETCH-LOGICAL-c447t-f24026fe1e1e6d91aea893829095832a54465994be92d2d824bcad1182fabb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19501371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2568866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bankson, D.D.</creatorcontrib><creatorcontrib>Rifai, N.</creatorcontrib><creatorcontrib>Williams, M.E.</creatorcontrib><creatorcontrib>Silverman, L.M.</creatorcontrib><creatorcontrib>Gray, T.K.</creatorcontrib><title>Biochemical effects of 17β-estradiol on UMR106 cells</title><title>Bone and mineral</title><addtitle>Bone Miner</addtitle><description>The effect of 17β-estradiol (E) on an osteoblast-like cell line, UMR106, was studied in vitro. The concentrations of transferrin and seven enzymes (gamma glutamyl transferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, alanine aminotransferase and aspettate aminotransferase) were measured in these cells after incubation in culture medium containing either E or the vehicle. E treatment increased five of the seven enzymes and increased the transferrin concentration in the UMR106 cells white simultaneously reducing the proliferation rates, 4-Hydroxytamoxifen, an estrogen antagonist, produced a mild estrogen agonist action on growth rates and enzyme concentrations in the UMR106 cells, When E was present simultaneously, the agonist properties of 4-hydroxytamoxifen were enhanced. These studies show that E enhanced activity of five enzymes and the transferrin content of UMR106 cells after a 2-day incubation. 4-Hydroxytamoxifen enhanced the E effect, illustrating that estrogen antagonists may manifest agonist or antagonist properties depending on the model. These results extend our previous observations showing a direct effect of E in vitro on osteoblast-like cells.</description><subject>4-Hydroxytamoxifen</subject><subject>Acid Phosphatase - metabolism</subject><subject>Alanine Transaminase - metabolism</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Creatine Kinase - metabolism</subject><subject>Enzyme</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen</subject><subject>Estrogen Antagonists - metabolism</subject><subject>Estrogen Antagonists - pharmacology</subject><subject>Estrogens - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Glutamyltransferase - metabolism</subject><subject>Hormone metabolism and regulation</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Mammalian female genital system</subject><subject>Osteoblastic cell</subject><subject>Osteoblasts - analysis</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - enzymology</subject><subject>Rats</subject><subject>Receptors, Estrogen - drug effects</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Tamoxifen - analogs & derivatives</subject><subject>Tamoxifen - metabolism</subject><subject>Tamoxifen - pharmacology</subject><subject>Transferrin</subject><subject>Transferrin - analysis</subject><subject>Vertebrates: reproduction</subject><issn>0169-6009</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN1KxDAQhXOhrOvqGyj0RtGLapKmaXIj6OIfrAiyXoc0PxhpmzXZFXwtH8RnMnXLeqcMw8DMmcPhA-AAwTMEET1PzXMKIT9h_JRDiIucbYHxZr0DdmN8TXtGCB6BES4pY5SOQXnlvHoxrVOyyYy1Ri1j5m2Gqq_P3MRlkNr5JvNd9vzwhCDNlGmauAe2rWyi2R_mBMxvrufTu3z2eHs_vZzlipBqmVtMIKbWoFRUcySNZLxgmENesgLLkhBack5qw7HGmmFSK6kRYtjKuobFBByvbRfBv61SGtG62AeQnfGrKCqOUEVZ8a8QlZhTUtAkJGuhCj7GYKxYBNfK8CEQFD1J0SMTPTLBuPghKVh6Oxz8V3Vr9OZpwJjuR8NdxgTSBtkpF3-9eQlRUaGku1jrTIL27kwQUTnTKaNdSOSF9u7vIN-HG47g</recordid><startdate>19890401</startdate><enddate>19890401</enddate><creator>Bankson, D.D.</creator><creator>Rifai, N.</creator><creator>Williams, M.E.</creator><creator>Silverman, L.M.</creator><creator>Gray, T.K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19890401</creationdate><title>Biochemical effects of 17β-estradiol on UMR106 cells</title><author>Bankson, D.D. ; Rifai, N. ; Williams, M.E. ; Silverman, L.M. ; Gray, T.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-f24026fe1e1e6d91aea893829095832a54465994be92d2d824bcad1182fabb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>4-Hydroxytamoxifen</topic><topic>Acid Phosphatase - metabolism</topic><topic>Alanine Transaminase - metabolism</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Aspartate Aminotransferases - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Creatine Kinase - metabolism</topic><topic>Enzyme</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen</topic><topic>Estrogen Antagonists - metabolism</topic><topic>Estrogen Antagonists - pharmacology</topic><topic>Estrogens - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Glutamyltransferase - metabolism</topic><topic>Hormone metabolism and regulation</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Mammalian female genital system</topic><topic>Osteoblastic cell</topic><topic>Osteoblasts - analysis</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - enzymology</topic><topic>Rats</topic><topic>Receptors, Estrogen - drug effects</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Tamoxifen - analogs & derivatives</topic><topic>Tamoxifen - metabolism</topic><topic>Tamoxifen - pharmacology</topic><topic>Transferrin</topic><topic>Transferrin - analysis</topic><topic>Vertebrates: reproduction</topic><toplevel>online_resources</toplevel><creatorcontrib>Bankson, D.D.</creatorcontrib><creatorcontrib>Rifai, N.</creatorcontrib><creatorcontrib>Williams, M.E.</creatorcontrib><creatorcontrib>Silverman, L.M.</creatorcontrib><creatorcontrib>Gray, T.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bone and mineral</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bankson, D.D.</au><au>Rifai, N.</au><au>Williams, M.E.</au><au>Silverman, L.M.</au><au>Gray, T.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical effects of 17β-estradiol on UMR106 cells</atitle><jtitle>Bone and mineral</jtitle><addtitle>Bone Miner</addtitle><date>1989-04-01</date><risdate>1989</risdate><volume>6</volume><issue>1</issue><spage>55</spage><epage>63</epage><pages>55-63</pages><issn>0169-6009</issn><coden>BOMIET</coden><abstract>The effect of 17β-estradiol (E) on an osteoblast-like cell line, UMR106, was studied in vitro. The concentrations of transferrin and seven enzymes (gamma glutamyl transferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, alanine aminotransferase and aspettate aminotransferase) were measured in these cells after incubation in culture medium containing either E or the vehicle. E treatment increased five of the seven enzymes and increased the transferrin concentration in the UMR106 cells white simultaneously reducing the proliferation rates, 4-Hydroxytamoxifen, an estrogen antagonist, produced a mild estrogen agonist action on growth rates and enzyme concentrations in the UMR106 cells, When E was present simultaneously, the agonist properties of 4-hydroxytamoxifen were enhanced. These studies show that E enhanced activity of five enzymes and the transferrin content of UMR106 cells after a 2-day incubation. 4-Hydroxytamoxifen enhanced the E effect, illustrating that estrogen antagonists may manifest agonist or antagonist properties depending on the model. These results extend our previous observations showing a direct effect of E in vitro on osteoblast-like cells.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2568866</pmid><doi>10.1016/0169-6009(89)90023-8</doi><tpages>9</tpages></addata></record> |
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| subjects | 4-Hydroxytamoxifen Acid Phosphatase - metabolism Alanine Transaminase - metabolism Alkaline Phosphatase - metabolism Animals Aspartate Aminotransferases - metabolism Biological and medical sciences Cell Differentiation - drug effects Cell Line Cells, Cultured Creatine Kinase - metabolism Enzyme Estradiol - pharmacology Estrogen Estrogen Antagonists - metabolism Estrogen Antagonists - pharmacology Estrogens - pharmacology Fundamental and applied biological sciences. Psychology gamma-Glutamyltransferase - metabolism Hormone metabolism and regulation L-Lactate Dehydrogenase - metabolism Mammalian female genital system Osteoblastic cell Osteoblasts - analysis Osteoblasts - drug effects Osteoblasts - enzymology Rats Receptors, Estrogen - drug effects Receptors, Estrogen - metabolism Tamoxifen - analogs & derivatives Tamoxifen - metabolism Tamoxifen - pharmacology Transferrin Transferrin - analysis Vertebrates: reproduction |
| title | Biochemical effects of 17β-estradiol on UMR106 cells |
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