The effects of donor stage on the survival and function of embryonic striatal grafts in the adult rat brain: II. Correlation between positron emission tomography and reaching behaviour
Grafts of embryonic striatal primordia are able to elicit behavioural recovery in rats which have received an excitotoxic lesion to the striatum, and it is believed that the P zones or striatal-like tissue within the transplants play a crucial role in these functional effects. We performed this stud...
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Veröffentlicht in: | Neuroscience 1997-08, Vol.79 (3), p.711-721 |
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creator | Fricker, R.A Torres, E.M Hume, S.P Myers, R Opacka-Juffrey, J Ashworth, S Brooks, D.J Dunnett, S.B |
description | Grafts of embryonic striatal primordia are able to elicit behavioural recovery in rats which have received an excitotoxic lesion to the striatum, and it is believed that the P zones or striatal-like tissue within the transplants play a crucial role in these functional effects. We performed this study to compare the effects of different donor stage of embryonic tissue on both the morphology (see accompanying paper) and function of striatal transplants. Both the medial and lateral ganglionic eminence was dissected from rat embryos of either 10
mm, 15
mm, 19
mm, or 23
mm crown-rump length, and implanted as a cell suspension into adult rats which had received an ibotenic acid lesion 10 days prior to transplantation. After four months the animals were tested on the “staircase task” of skilled forelimb use. At 10–14 months rats from the groups which had received grafts from 10
mm or 15
mm donor embryos were taken for positron emission tomography scanning in a small diameter postiron emission tomography scanner, using ligands to the dopamine D
1 and D
2 receptors, [
11C]SCH 23390 and [
11C]raclopride, respectively. A lesion-alone group was also scanned with the same ligands for comparison. Animals which had received transplants from the 10
mm donors showed a significant recovery with their contralateral paw on the “staircase test”. No other groups showed recovery on this task. Similarly, the animals with grafts from the youngest donors showed a significant increase in D
1 and D
2 receptor binding when compared to the lesion-alone group. No increase in signal was observed with either ligand in the group which had received grafts from 15
mm donors. Success in paw reaching showed a strong correlation to both the positron emission tomography signal obtained and the P zone volume of the grafts.
These results suggest that striatal grafts from younger donors (10
mm CRL) give greater behavioural recovery than grafts prepared from older embryos. This recovery is due to both the increased proportion of striatal-like tissue within the grafts and an increase in functional D
1 and D
2 dopamine receptors measured by positron emission tomography, i.e. a more extensive integration of the graft with the host brain. |
doi_str_mv | 10.1016/S0306-4522(96)00657-4 |
format | Article |
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mm, 15
mm, 19
mm, or 23
mm crown-rump length, and implanted as a cell suspension into adult rats which had received an ibotenic acid lesion 10 days prior to transplantation. After four months the animals were tested on the “staircase task” of skilled forelimb use. At 10–14 months rats from the groups which had received grafts from 10
mm or 15
mm donor embryos were taken for positron emission tomography scanning in a small diameter postiron emission tomography scanner, using ligands to the dopamine D
1 and D
2 receptors, [
11C]SCH 23390 and [
11C]raclopride, respectively. A lesion-alone group was also scanned with the same ligands for comparison. Animals which had received transplants from the 10
mm donors showed a significant recovery with their contralateral paw on the “staircase test”. No other groups showed recovery on this task. Similarly, the animals with grafts from the youngest donors showed a significant increase in D
1 and D
2 receptor binding when compared to the lesion-alone group. No increase in signal was observed with either ligand in the group which had received grafts from 15
mm donors. Success in paw reaching showed a strong correlation to both the positron emission tomography signal obtained and the P zone volume of the grafts.
These results suggest that striatal grafts from younger donors (10
mm CRL) give greater behavioural recovery than grafts prepared from older embryos. This recovery is due to both the increased proportion of striatal-like tissue within the grafts and an increase in functional D
1 and D
2 dopamine receptors measured by positron emission tomography, i.e. a more extensive integration of the graft with the host brain.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/S0306-4522(96)00657-4</identifier><identifier>PMID: 9219935</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Behavior, Animal - physiology ; Biological and medical sciences ; Brain - diagnostic imaging ; Brain Tissue Transplantation ; Cell Survival - physiology ; Corpus Striatum - transplantation ; Development. Senescence. Regeneration. Transplantation ; dopamine receptors ; Embryo Transfer ; embryonic striatal grafts ; Female ; Fundamental and applied biological sciences. Psychology ; paw reaching ; positron emission tomography ; raclopride ; Rats ; Rats, Sprague-Dawley ; SCH 23390 ; Time Factors ; Tomography, Emission-Computed ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 1997-08, Vol.79 (3), p.711-721</ispartof><rights>1997 IBRO</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0306-4522(96)00657-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2712261$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9219935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fricker, R.A</creatorcontrib><creatorcontrib>Torres, E.M</creatorcontrib><creatorcontrib>Hume, S.P</creatorcontrib><creatorcontrib>Myers, R</creatorcontrib><creatorcontrib>Opacka-Juffrey, J</creatorcontrib><creatorcontrib>Ashworth, S</creatorcontrib><creatorcontrib>Brooks, D.J</creatorcontrib><creatorcontrib>Dunnett, S.B</creatorcontrib><title>The effects of donor stage on the survival and function of embryonic striatal grafts in the adult rat brain: II. Correlation between positron emission tomography and reaching behaviour</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Grafts of embryonic striatal primordia are able to elicit behavioural recovery in rats which have received an excitotoxic lesion to the striatum, and it is believed that the P zones or striatal-like tissue within the transplants play a crucial role in these functional effects. We performed this study to compare the effects of different donor stage of embryonic tissue on both the morphology (see accompanying paper) and function of striatal transplants. Both the medial and lateral ganglionic eminence was dissected from rat embryos of either 10
mm, 15
mm, 19
mm, or 23
mm crown-rump length, and implanted as a cell suspension into adult rats which had received an ibotenic acid lesion 10 days prior to transplantation. After four months the animals were tested on the “staircase task” of skilled forelimb use. At 10–14 months rats from the groups which had received grafts from 10
mm or 15
mm donor embryos were taken for positron emission tomography scanning in a small diameter postiron emission tomography scanner, using ligands to the dopamine D
1 and D
2 receptors, [
11C]SCH 23390 and [
11C]raclopride, respectively. A lesion-alone group was also scanned with the same ligands for comparison. Animals which had received transplants from the 10
mm donors showed a significant recovery with their contralateral paw on the “staircase test”. No other groups showed recovery on this task. Similarly, the animals with grafts from the youngest donors showed a significant increase in D
1 and D
2 receptor binding when compared to the lesion-alone group. No increase in signal was observed with either ligand in the group which had received grafts from 15
mm donors. Success in paw reaching showed a strong correlation to both the positron emission tomography signal obtained and the P zone volume of the grafts.
These results suggest that striatal grafts from younger donors (10
mm CRL) give greater behavioural recovery than grafts prepared from older embryos. This recovery is due to both the increased proportion of striatal-like tissue within the grafts and an increase in functional D
1 and D
2 dopamine receptors measured by positron emission tomography, i.e. a more extensive integration of the graft with the host brain.</description><subject>Animals</subject><subject>Behavior, Animal - physiology</subject><subject>Biological and medical sciences</subject><subject>Brain - diagnostic imaging</subject><subject>Brain Tissue Transplantation</subject><subject>Cell Survival - physiology</subject><subject>Corpus Striatum - transplantation</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>dopamine receptors</subject><subject>Embryo Transfer</subject><subject>embryonic striatal grafts</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>paw reaching</subject><subject>positron emission tomography</subject><subject>raclopride</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>SCH 23390</subject><subject>Time Factors</subject><subject>Tomography, Emission-Computed</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksuOFCEUhitGM_aMPsIkLIzRRY1AcWncGNPx0skkLhzXhKIO3ZgqaIHqSb-Zjyd9yWxlQ8L_nR8452-aW4LvCCbiw0_cYdEyTuk7Jd5jLLhs2bNmQZayayVn7HmzeEJeNtc5_8Z1cdZdNVeKEqU6vmj-PmwBgXNgS0bRoSGGmFAuZgMoBlSqmue093szIhMG5OZgi69KZWHq0yEGbyufvCkV2STjqpE_V5phHgtKpqA-GR8-ovX6Dq1iSjCak0kP5REgoF3MvqR6AJPP-aiUOMVqttseTtcmMHbrw6ZWbM3exzm9al44M2Z4fdlvml9fvzysvrf3P76tV5_vW6BClJZiLlxthsJM8GXHHbXEDrVHxEgAKRnul9j11lkHQ09oZxQzVHLuOoUHkN1N8_bsu0vxzwy56PpEC-NoAsQ5a6kI4VyJ_4JEMMGIOIK3F3DuJxj0LvnJpIO-zKTqby66ydaMLplgfX7CqCSUClKxT2cM6u_3HpLO1kOwMPhUp6mH6DXB-pgVfcqKPgZBK6FPWdGs-wcn8LNx</recordid><startdate>199708</startdate><enddate>199708</enddate><creator>Fricker, R.A</creator><creator>Torres, E.M</creator><creator>Hume, S.P</creator><creator>Myers, R</creator><creator>Opacka-Juffrey, J</creator><creator>Ashworth, S</creator><creator>Brooks, D.J</creator><creator>Dunnett, S.B</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199708</creationdate><title>The effects of donor stage on the survival and function of embryonic striatal grafts in the adult rat brain: II. Correlation between positron emission tomography and reaching behaviour</title><author>Fricker, R.A ; Torres, E.M ; Hume, S.P ; Myers, R ; Opacka-Juffrey, J ; Ashworth, S ; Brooks, D.J ; Dunnett, S.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e266t-2056f54490465835f2c1cd1871a7ee7740b80fbcfcfedb123a94a2755f390de73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Behavior, Animal - physiology</topic><topic>Biological and medical sciences</topic><topic>Brain - diagnostic imaging</topic><topic>Brain Tissue Transplantation</topic><topic>Cell Survival - physiology</topic><topic>Corpus Striatum - transplantation</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>dopamine receptors</topic><topic>Embryo Transfer</topic><topic>embryonic striatal grafts</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>paw reaching</topic><topic>positron emission tomography</topic><topic>raclopride</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>SCH 23390</topic><topic>Time Factors</topic><topic>Tomography, Emission-Computed</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fricker, R.A</creatorcontrib><creatorcontrib>Torres, E.M</creatorcontrib><creatorcontrib>Hume, S.P</creatorcontrib><creatorcontrib>Myers, R</creatorcontrib><creatorcontrib>Opacka-Juffrey, J</creatorcontrib><creatorcontrib>Ashworth, S</creatorcontrib><creatorcontrib>Brooks, D.J</creatorcontrib><creatorcontrib>Dunnett, S.B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fricker, R.A</au><au>Torres, E.M</au><au>Hume, S.P</au><au>Myers, R</au><au>Opacka-Juffrey, J</au><au>Ashworth, S</au><au>Brooks, D.J</au><au>Dunnett, S.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of donor stage on the survival and function of embryonic striatal grafts in the adult rat brain: II. Correlation between positron emission tomography and reaching behaviour</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1997-08</date><risdate>1997</risdate><volume>79</volume><issue>3</issue><spage>711</spage><epage>721</epage><pages>711-721</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Grafts of embryonic striatal primordia are able to elicit behavioural recovery in rats which have received an excitotoxic lesion to the striatum, and it is believed that the P zones or striatal-like tissue within the transplants play a crucial role in these functional effects. We performed this study to compare the effects of different donor stage of embryonic tissue on both the morphology (see accompanying paper) and function of striatal transplants. Both the medial and lateral ganglionic eminence was dissected from rat embryos of either 10
mm, 15
mm, 19
mm, or 23
mm crown-rump length, and implanted as a cell suspension into adult rats which had received an ibotenic acid lesion 10 days prior to transplantation. After four months the animals were tested on the “staircase task” of skilled forelimb use. At 10–14 months rats from the groups which had received grafts from 10
mm or 15
mm donor embryos were taken for positron emission tomography scanning in a small diameter postiron emission tomography scanner, using ligands to the dopamine D
1 and D
2 receptors, [
11C]SCH 23390 and [
11C]raclopride, respectively. A lesion-alone group was also scanned with the same ligands for comparison. Animals which had received transplants from the 10
mm donors showed a significant recovery with their contralateral paw on the “staircase test”. No other groups showed recovery on this task. Similarly, the animals with grafts from the youngest donors showed a significant increase in D
1 and D
2 receptor binding when compared to the lesion-alone group. No increase in signal was observed with either ligand in the group which had received grafts from 15
mm donors. Success in paw reaching showed a strong correlation to both the positron emission tomography signal obtained and the P zone volume of the grafts.
These results suggest that striatal grafts from younger donors (10
mm CRL) give greater behavioural recovery than grafts prepared from older embryos. This recovery is due to both the increased proportion of striatal-like tissue within the grafts and an increase in functional D
1 and D
2 dopamine receptors measured by positron emission tomography, i.e. a more extensive integration of the graft with the host brain.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9219935</pmid><doi>10.1016/S0306-4522(96)00657-4</doi><tpages>11</tpages></addata></record> |
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subjects | Animals Behavior, Animal - physiology Biological and medical sciences Brain - diagnostic imaging Brain Tissue Transplantation Cell Survival - physiology Corpus Striatum - transplantation Development. Senescence. Regeneration. Transplantation dopamine receptors Embryo Transfer embryonic striatal grafts Female Fundamental and applied biological sciences. Psychology paw reaching positron emission tomography raclopride Rats Rats, Sprague-Dawley SCH 23390 Time Factors Tomography, Emission-Computed Vertebrates: nervous system and sense organs |
title | The effects of donor stage on the survival and function of embryonic striatal grafts in the adult rat brain: II. Correlation between positron emission tomography and reaching behaviour |
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