Evaluation of hepatitis C virus envelope proteins expressed in E. coli and insect cells for use as tools for antibody screening

Background/Methods: The two envelope proteins of hepatitis C virus, E1 and E2, were expressed in E. coli and, as secretory proteins, in Sf9 insect cells using recombinant baculoviruses. Co-infection of insect cells with E1 and E2-recombinant baculoviruses was performed, which has been shown to resul...

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Veröffentlicht in:	Journal of hepatology 1997-06, Vol.26 (6), p.1179-1186
Hauptverfasser:	Hüssy, Peter, Faust, Heinrich, Wagner, Jean-Claude, Schmid, Georg, Mous, Jan, Jacobsen, Helmut
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Line Cloning, Molecular Dimerization Escherichia coli Hepacivirus - immunology Hepatitis C - diagnosis Hepatitis C - prevention & control Hepatitis C Antibodies - blood Human viral diseases Humans Immunoenzyme Techniques Infectious diseases Mass Screening Medical sciences Recombinant Proteins - biosynthesis Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Reference Values Reproducibility of Results Spodoptera Transfection Viral diseases Viral Envelope Proteins - biosynthesis Viral Envelope Proteins - immunology Viral Envelope Proteins - isolation & purification Viral hepatitis
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