[15] Determination, purification, and characterization of α-NADH and α-NADPH

This chapter describes the two methods for the quantitative determination of small amounts of α-anomers of nicotinamide adenine dinucleotide (NAD), NADH, nicotinamide adenine dinucleotide phosphate (NADP), and NADPH in the presence of large amounts of β-anomers. These methods use ion-exchange chroma...

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Veröffentlicht in:	Methods in Enzymology 1997, Vol.280, p.171-186
Hauptverfasser:	Klemm, Andree, Steiner, Thomas, Flötgen, Uwe, Cumme, Gerhard A., Horn, Anton
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid Chromatography, Ion Exchange Erythrocytes - chemistry Glutathione Reductase - metabolism Humans Models, Chemical NAD - chemistry NAD - metabolism NADP - chemistry NADP - metabolism Oxidation-Reduction Spectrophotometry Stereoisomerism Substrate Specificity
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