Von Willebrand factor restores impaired platelet thrombogenesis in copper-deficient rats
Dietary copper restriction reduces microvascular thrombogenesis. We have now examined the roles of shear forces and von Willebrand factor (vWF) in in vivo thrombus formation in the cremaster microcirculation of copper-deficient rats. Male weanling Sprague-Dawley rats were fed purified diets that wer...
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| Veröffentlicht in: | The Journal of nutrition 1997-07, Vol.127 (7), p.1320-1327 |
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| creator | Lominadze, David Saari, Jack T Miller, Frederick N Catalfamo, James L Schuschke, Dale A |
| description | Dietary copper restriction reduces microvascular thrombogenesis. We have now examined the roles of shear forces and von Willebrand factor (vWF) in in vivo thrombus formation in the cremaster microcirculation of copper-deficient rats. Male weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (6.3 mg Cu/kg) or copper-deficient (0.3 mg Cu/kg) for 4 wk. Intravascular fluorescein isothiocyanate tagged to bovine serum albumin was activated with 450-490 nm light to induce thrombus formation in microvessels. Thrombus initiation time was significantly prolonged in copper-deficient rats; after thrombus appearance, however, vessel occlusion was significantly accelerated. The greater shear rates of arterioles compared with venules significantly increased the thrombus initiation time in both groups. However, vessel occlusion time and thrombus growth time were independent of shear rate. Intravascular vWF (0.2 u/100 g body wt) decreased thrombus initiation time in the CuD group without affecting thrombus growth time. The data suggest that decreased thrombogenesis in copper-deficient rats is not a result of altered rheological factors or arteriolar-venular differences, but appears to result from decreased platelet-to-endothelial cell adhesion. |
| doi_str_mv | 10.1093/jn/127.7.1320 |
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We have now examined the roles of shear forces and von Willebrand factor (vWF) in in vivo thrombus formation in the cremaster microcirculation of copper-deficient rats. Male weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (6.3 mg Cu/kg) or copper-deficient (0.3 mg Cu/kg) for 4 wk. Intravascular fluorescein isothiocyanate tagged to bovine serum albumin was activated with 450-490 nm light to induce thrombus formation in microvessels. Thrombus initiation time was significantly prolonged in copper-deficient rats; after thrombus appearance, however, vessel occlusion was significantly accelerated. The greater shear rates of arterioles compared with venules significantly increased the thrombus initiation time in both groups. However, vessel occlusion time and thrombus growth time were independent of shear rate. Intravascular vWF (0.2 u/100 g body wt) decreased thrombus initiation time in the CuD group without affecting thrombus growth time. The data suggest that decreased thrombogenesis in copper-deficient rats is not a result of altered rheological factors or arteriolar-venular differences, but appears to result from decreased platelet-to-endothelial cell adhesion.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/127.7.1320</identifier><identifier>PMID: 9202086</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Nutritional Sciences</publisher><subject>Analysis of Variance ; animal models ; Animals ; arteries ; Arterioles - cytology ; Arterioles - physiology ; Biological and medical sciences ; Blood ; blood coagulation factors ; blood plasma ; blood platelets ; Blood Platelets - cytology ; Blood Platelets - drug effects ; Blood Platelets - physiology ; blood pressure ; Blood Pressure - physiology ; Blood Viscosity - physiology ; body weight ; Body Weight - physiology ; Cell Adhesion - drug effects ; Cell Adhesion - physiology ; Circulatory system ; Copper ; Copper - analysis ; Copper - deficiency ; Copper - physiology ; diet ; duration ; Endothelium, Vascular - cytology ; Endothelium, Vascular - physiology ; experimental diets ; heart rate ; Heart Rate - physiology ; Hematocrit ; Iron - analysis ; liver ; Liver - chemistry ; Male ; Medical sciences ; Metabolic diseases ; nutrient deficiencies ; Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) ; Rats ; Rats, Sprague-Dawley ; Regional Blood Flow ; Rodents ; shear ; thrombosis ; Thrombosis - blood ; Thrombosis - etiology ; Thrombosis - physiopathology ; trace element deficiencies ; veins ; viscosity ; von Willebrand Factor - pharmacology</subject><ispartof>The Journal of nutrition, 1997-07, Vol.127 (7), p.1320-1327</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright American Institute of Nutrition Jul 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-a9f7191db4986e0ebf283dfc48c1156ad356c315264e56c86c64ee13bba95adc3</citedby><cites>FETCH-LOGICAL-c407t-a9f7191db4986e0ebf283dfc48c1156ad356c315264e56c86c64ee13bba95adc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2762170$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9202086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lominadze, David</creatorcontrib><creatorcontrib>Saari, Jack T</creatorcontrib><creatorcontrib>Miller, Frederick N</creatorcontrib><creatorcontrib>Catalfamo, James L</creatorcontrib><creatorcontrib>Schuschke, Dale A</creatorcontrib><title>Von Willebrand factor restores impaired platelet thrombogenesis in copper-deficient rats</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>Dietary copper restriction reduces microvascular thrombogenesis. We have now examined the roles of shear forces and von Willebrand factor (vWF) in in vivo thrombus formation in the cremaster microcirculation of copper-deficient rats. Male weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (6.3 mg Cu/kg) or copper-deficient (0.3 mg Cu/kg) for 4 wk. Intravascular fluorescein isothiocyanate tagged to bovine serum albumin was activated with 450-490 nm light to induce thrombus formation in microvessels. Thrombus initiation time was significantly prolonged in copper-deficient rats; after thrombus appearance, however, vessel occlusion was significantly accelerated. The greater shear rates of arterioles compared with venules significantly increased the thrombus initiation time in both groups. However, vessel occlusion time and thrombus growth time were independent of shear rate. Intravascular vWF (0.2 u/100 g body wt) decreased thrombus initiation time in the CuD group without affecting thrombus growth time. The data suggest that decreased thrombogenesis in copper-deficient rats is not a result of altered rheological factors or arteriolar-venular differences, but appears to result from decreased platelet-to-endothelial cell adhesion.</description><subject>Analysis of Variance</subject><subject>animal models</subject><subject>Animals</subject><subject>arteries</subject><subject>Arterioles - cytology</subject><subject>Arterioles - physiology</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>blood coagulation factors</subject><subject>blood plasma</subject><subject>blood platelets</subject><subject>Blood Platelets - cytology</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - physiology</subject><subject>blood pressure</subject><subject>Blood Pressure - physiology</subject><subject>Blood Viscosity - physiology</subject><subject>body weight</subject><subject>Body Weight - physiology</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - physiology</subject><subject>Circulatory system</subject><subject>Copper</subject><subject>Copper - analysis</subject><subject>Copper - deficiency</subject><subject>Copper - physiology</subject><subject>diet</subject><subject>duration</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - physiology</subject><subject>experimental diets</subject><subject>heart rate</subject><subject>Heart Rate - physiology</subject><subject>Hematocrit</subject><subject>Iron - analysis</subject><subject>liver</subject><subject>Liver - chemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>nutrient deficiencies</subject><subject>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regional Blood Flow</subject><subject>Rodents</subject><subject>shear</subject><subject>thrombosis</subject><subject>Thrombosis - blood</subject><subject>Thrombosis - etiology</subject><subject>Thrombosis - physiopathology</subject><subject>trace element deficiencies</subject><subject>veins</subject><subject>viscosity</subject><subject>von Willebrand Factor - pharmacology</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEFv1DAQhS0EKtvCkSMiQhW3bGfsxI6PqGoBqRIHKO3Ncpxx8SqJg5098O_xalc9cJonzaenp4-xdwhbBC2udvMVcrVVWxQcXrANtg3WEgFesg0A57VAKV-z85x3AICN7s7YmebAoZMb9vgrztVDGEfqk52Hylu3xlQlyuVQrsK02JBoqJbRrjTSWq2_U5z6-EQz5VCAuXJxWSjVA_ngAs1rleya37BX3o6Z3p7uBbu_vfl5_bW--_7l2_Xnu9o1oNbaaq9Q49CXXZKAes87MXjXdA6xlXYQrXQCWy4bKqmTrgRC0fdWt3Zw4oJ9OvYuKf7Zl9lmCtnRONqZ4j4bpYsllFDAj_-Bu7hPc9lmUKtGKJCiQPURcinmnMibJYXJpr8GwRx0m91sim6jzEF34d-fSvf9RMMzffJb_penv83Ojr44diE_Y1xJjupQ8-GIeRuNfUoFuf_BAQXwTrdtw8U_2vGRVQ</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Lominadze, David</creator><creator>Saari, Jack T</creator><creator>Miller, Frederick N</creator><creator>Catalfamo, James L</creator><creator>Schuschke, Dale A</creator><general>American Society for Nutritional Sciences</general><general>American Institute of Nutrition</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>19970701</creationdate><title>Von Willebrand factor restores impaired platelet thrombogenesis in copper-deficient rats</title><author>Lominadze, David ; Saari, Jack T ; Miller, Frederick N ; Catalfamo, James L ; Schuschke, Dale A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-a9f7191db4986e0ebf283dfc48c1156ad356c315264e56c86c64ee13bba95adc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Analysis of Variance</topic><topic>animal models</topic><topic>Animals</topic><topic>arteries</topic><topic>Arterioles - cytology</topic><topic>Arterioles - physiology</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>blood coagulation factors</topic><topic>blood plasma</topic><topic>blood platelets</topic><topic>Blood Platelets - cytology</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - physiology</topic><topic>blood pressure</topic><topic>Blood Pressure - physiology</topic><topic>Blood Viscosity - physiology</topic><topic>body weight</topic><topic>Body Weight - physiology</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Adhesion - physiology</topic><topic>Circulatory system</topic><topic>Copper</topic><topic>Copper - analysis</topic><topic>Copper - deficiency</topic><topic>Copper - physiology</topic><topic>diet</topic><topic>duration</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - physiology</topic><topic>experimental diets</topic><topic>heart rate</topic><topic>Heart Rate - physiology</topic><topic>Hematocrit</topic><topic>Iron - analysis</topic><topic>liver</topic><topic>Liver - chemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>nutrient deficiencies</topic><topic>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regional Blood Flow</topic><topic>Rodents</topic><topic>shear</topic><topic>thrombosis</topic><topic>Thrombosis - blood</topic><topic>Thrombosis - etiology</topic><topic>Thrombosis - physiopathology</topic><topic>trace element deficiencies</topic><topic>veins</topic><topic>viscosity</topic><topic>von Willebrand Factor - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lominadze, David</creatorcontrib><creatorcontrib>Saari, Jack T</creatorcontrib><creatorcontrib>Miller, Frederick N</creatorcontrib><creatorcontrib>Catalfamo, James L</creatorcontrib><creatorcontrib>Schuschke, Dale A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lominadze, David</au><au>Saari, Jack T</au><au>Miller, Frederick N</au><au>Catalfamo, James L</au><au>Schuschke, Dale A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Von Willebrand factor restores impaired platelet thrombogenesis in copper-deficient rats</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>127</volume><issue>7</issue><spage>1320</spage><epage>1327</epage><pages>1320-1327</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>Dietary copper restriction reduces microvascular thrombogenesis. We have now examined the roles of shear forces and von Willebrand factor (vWF) in in vivo thrombus formation in the cremaster microcirculation of copper-deficient rats. Male weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (6.3 mg Cu/kg) or copper-deficient (0.3 mg Cu/kg) for 4 wk. Intravascular fluorescein isothiocyanate tagged to bovine serum albumin was activated with 450-490 nm light to induce thrombus formation in microvessels. Thrombus initiation time was significantly prolonged in copper-deficient rats; after thrombus appearance, however, vessel occlusion was significantly accelerated. The greater shear rates of arterioles compared with venules significantly increased the thrombus initiation time in both groups. However, vessel occlusion time and thrombus growth time were independent of shear rate. Intravascular vWF (0.2 u/100 g body wt) decreased thrombus initiation time in the CuD group without affecting thrombus growth time. The data suggest that decreased thrombogenesis in copper-deficient rats is not a result of altered rheological factors or arteriolar-venular differences, but appears to result from decreased platelet-to-endothelial cell adhesion.</abstract><cop>Bethesda, MD</cop><pub>American Society for Nutritional Sciences</pub><pmid>9202086</pmid><doi>10.1093/jn/127.7.1320</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of nutrition, 1997-07, Vol.127 (7), p.1320-1327 |
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| subjects | Analysis of Variance animal models Animals arteries Arterioles - cytology Arterioles - physiology Biological and medical sciences Blood blood coagulation factors blood plasma blood platelets Blood Platelets - cytology Blood Platelets - drug effects Blood Platelets - physiology blood pressure Blood Pressure - physiology Blood Viscosity - physiology body weight Body Weight - physiology Cell Adhesion - drug effects Cell Adhesion - physiology Circulatory system Copper Copper - analysis Copper - deficiency Copper - physiology diet duration Endothelium, Vascular - cytology Endothelium, Vascular - physiology experimental diets heart rate Heart Rate - physiology Hematocrit Iron - analysis liver Liver - chemistry Male Medical sciences Metabolic diseases nutrient deficiencies Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) Rats Rats, Sprague-Dawley Regional Blood Flow Rodents shear thrombosis Thrombosis - blood Thrombosis - etiology Thrombosis - physiopathology trace element deficiencies veins viscosity von Willebrand Factor - pharmacology |
| title | Von Willebrand factor restores impaired platelet thrombogenesis in copper-deficient rats |
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