Acid pH Induces Formation of Dense Cells in Sickle Erythrocytes
When erythrocytes from patients homozygous for hemoglobin S (SS) are swollen or exposed to pH
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| Veröffentlicht in: | Blood 1989-07, Vol.74 (1), p.487-495 |
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| container_title | Blood |
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| creator | Brugnara, Carlo Van Ha, Thuong Tosteson, Daniel C. |
| description | When erythrocytes from patients homozygous for hemoglobin S (SS) are swollen or exposed to pH |
| doi_str_mv | 10.1182/blood.V74.1.487.487 |
| format | Article |
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We report that carbon monoxidetreated SS cells become progressively denser when incubated for eight to 12 hours in media with pH <7.40 (7.3 to 7.0) at constant cell 2,3-diphosphoglycerate (DPG). This phenomenon is maximal in fresh SS cells from the top and middle density fractions, and is absent in cells from the densest fraction. When AA cells are separated according to density, acid pH induces cell shrinkage in the least dense fraction of AA cells, which has considerable KCI cotransport, but produces no change in cell density in the densest fractions of AA erythrocyte, which have no KCI cotransport. These data suggest that dense cells can form in oxygenated SS erythrocytes when the KCI cotransport system is activated by acidification.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V74.1.487.487</identifier><identifier>PMID: 2752126</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Anemia, Sickle Cell - blood ; Anemias. Hemoglobinopathies ; Biological and medical sciences ; Biological Transport ; Centrifugation, Density Gradient ; Chlorides - blood ; Diseases of red blood cells ; Erythrocytes, Abnormal - physiopathology ; Hematologic and hematopoietic diseases ; Humans ; Hydrogen-Ion Concentration ; In Vitro Techniques ; Medical sciences ; Potassium - blood ; Water-Electrolyte Balance</subject><ispartof>Blood, 1989-07, Vol.74 (1), p.487-495</ispartof><rights>1989 American Society of Hematology</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-5c62a22c4aa93f3037dfbeaada6f98e10d9d2f26f36d4ea1c0599ddf5541b4df3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7317038$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2752126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brugnara, Carlo</creatorcontrib><creatorcontrib>Van Ha, Thuong</creatorcontrib><creatorcontrib>Tosteson, Daniel C.</creatorcontrib><title>Acid pH Induces Formation of Dense Cells in Sickle Erythrocytes</title><title>Blood</title><addtitle>Blood</addtitle><description>When erythrocytes from patients homozygous for hemoglobin S (SS) are swollen or exposed to pH <7.40, they lose K, Cl, and water through a volume and pH-dependent KCI cotransport system. We report that carbon monoxidetreated SS cells become progressively denser when incubated for eight to 12 hours in media with pH <7.40 (7.3 to 7.0) at constant cell 2,3-diphosphoglycerate (DPG). This phenomenon is maximal in fresh SS cells from the top and middle density fractions, and is absent in cells from the densest fraction. When AA cells are separated according to density, acid pH induces cell shrinkage in the least dense fraction of AA cells, which has considerable KCI cotransport, but produces no change in cell density in the densest fractions of AA erythrocyte, which have no KCI cotransport. These data suggest that dense cells can form in oxygenated SS erythrocytes when the KCI cotransport system is activated by acidification.</description><subject>Anemia, Sickle Cell - blood</subject><subject>Anemias. Hemoglobinopathies</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Centrifugation, Density Gradient</subject><subject>Chlorides - blood</subject><subject>Diseases of red blood cells</subject><subject>Erythrocytes, Abnormal - physiopathology</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>In Vitro Techniques</subject><subject>Medical sciences</subject><subject>Potassium - blood</subject><subject>Water-Electrolyte Balance</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotVafQIQsxN3U3Oa2ECm11ULBhZdtyCQnGJ1OajIj9O2d2tKli8NZnO_8_HwIXVIyprRgt1XtvRm_52JMx6LIt3OEhjRlRUIII8doSAjJElHm9BSdxfhJCBWcpQM0YHnKKMuG6H6incHrJ7xoTKch4rkPK9U632Bv8QM0EfAU6jpi1-AXp79qwLOwaT-C15sW4jk6saqOcLHfI_Q2n71On5Ll8-NiOlkmmmekTVKdMcWYFkqV3HLCc2MrUMqozJYFUGJKwyzLLM-MAEU1ScvSGJumglbCWD5CN7vcdfDfHcRWrlzUfTHVgO-izEtKRJHyHuQ7UAcfYwAr18GtVNhISuRWm_zTJnttkspe2Xb6r6t9fFetwBx-9p76-_X-rqJWtQ2q0S4esJzTnPCix-52GPQqfhwEGbWDRoNxAXQrjXf_1vgFT9qLaw</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Brugnara, Carlo</creator><creator>Van Ha, Thuong</creator><creator>Tosteson, Daniel C.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890701</creationdate><title>Acid pH Induces Formation of Dense Cells in Sickle Erythrocytes</title><author>Brugnara, Carlo ; Van Ha, Thuong ; Tosteson, Daniel C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-5c62a22c4aa93f3037dfbeaada6f98e10d9d2f26f36d4ea1c0599ddf5541b4df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Anemia, Sickle Cell - blood</topic><topic>Anemias. Hemoglobinopathies</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Centrifugation, Density Gradient</topic><topic>Chlorides - blood</topic><topic>Diseases of red blood cells</topic><topic>Erythrocytes, Abnormal - physiopathology</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>In Vitro Techniques</topic><topic>Medical sciences</topic><topic>Potassium - blood</topic><topic>Water-Electrolyte Balance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brugnara, Carlo</creatorcontrib><creatorcontrib>Van Ha, Thuong</creatorcontrib><creatorcontrib>Tosteson, Daniel C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brugnara, Carlo</au><au>Van Ha, Thuong</au><au>Tosteson, Daniel C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acid pH Induces Formation of Dense Cells in Sickle Erythrocytes</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>74</volume><issue>1</issue><spage>487</spage><epage>495</epage><pages>487-495</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>When erythrocytes from patients homozygous for hemoglobin S (SS) are swollen or exposed to pH <7.40, they lose K, Cl, and water through a volume and pH-dependent KCI cotransport system. We report that carbon monoxidetreated SS cells become progressively denser when incubated for eight to 12 hours in media with pH <7.40 (7.3 to 7.0) at constant cell 2,3-diphosphoglycerate (DPG). This phenomenon is maximal in fresh SS cells from the top and middle density fractions, and is absent in cells from the densest fraction. When AA cells are separated according to density, acid pH induces cell shrinkage in the least dense fraction of AA cells, which has considerable KCI cotransport, but produces no change in cell density in the densest fractions of AA erythrocyte, which have no KCI cotransport. These data suggest that dense cells can form in oxygenated SS erythrocytes when the KCI cotransport system is activated by acidification.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>2752126</pmid><doi>10.1182/blood.V74.1.487.487</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Blood, 1989-07, Vol.74 (1), p.487-495 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Anemia, Sickle Cell - blood Anemias. Hemoglobinopathies Biological and medical sciences Biological Transport Centrifugation, Density Gradient Chlorides - blood Diseases of red blood cells Erythrocytes, Abnormal - physiopathology Hematologic and hematopoietic diseases Humans Hydrogen-Ion Concentration In Vitro Techniques Medical sciences Potassium - blood Water-Electrolyte Balance |
| title | Acid pH Induces Formation of Dense Cells in Sickle Erythrocytes |
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