Quantitation of Vascular Endothelial Growth Factor mRNA Levels in Human Breast Tumors and Metastatic Lymph Nodes
In situhybridization analysis provides a means to qualitatively study the heterogeneity of primary tumors and metastases based on the types of genes transcribed. In this study, we have tested some parameters for quantitative analysis ofin situhybridizations with paraffin-embedded human breast tumors...
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| Veröffentlicht in: | Experimental and molecular pathology 1997-02, Vol.64 (1), p.41-51 |
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| container_title | Experimental and molecular pathology |
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| creator | Wright, Paul S. Loudy, David E. Cross-Doersen, Doreen E. Montgomery, Lauren R. Sprinkle-Cavallo, Jean Miller, Jerry A. Distler, Carole M. Lower, Elyse E. Woessner, Richard D. |
| description | In situhybridization analysis provides a means to qualitatively study the heterogeneity of primary tumors and metastases based on the types of genes transcribed. In this study, we have tested some parameters for quantitative analysis ofin situhybridizations with paraffin-embedded human breast tumors and measured mRNA levels for the angiogenic protein, vascular endothelial growth factor (VEGF). VEGF mRNAs were highly tumor specific, with the highest levels near necrotic regions within the tissues (0.1 to 2.7 dpm/mm2). Normal cells within the tissue sections did not have detectable levels of VEGF mRNA. For comparison, tumor levels of c-myc(4 to 46 dpm/mm2) and glyceraldehyde-3-phosphate dehydrogenase mRNAs (48 to 214 dpm/mm2) were measured. The mRNAs for both of these genes were more broadly expressed across the tissue sections. The hybridization pattern for VEGF mRNAs was consistent with hypoxia-induced VEGF mRNA steady-state levels and supports the hypothesis that oxidative stress regulates VEGF expression in breast tumors. |
| doi_str_mv | 10.1006/exmp.1997.2208 |
| format | Article |
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In this study, we have tested some parameters for quantitative analysis ofin situhybridizations with paraffin-embedded human breast tumors and measured mRNA levels for the angiogenic protein, vascular endothelial growth factor (VEGF). VEGF mRNAs were highly tumor specific, with the highest levels near necrotic regions within the tissues (0.1 to 2.7 dpm/mm2). Normal cells within the tissue sections did not have detectable levels of VEGF mRNA. For comparison, tumor levels of c-myc(4 to 46 dpm/mm2) and glyceraldehyde-3-phosphate dehydrogenase mRNAs (48 to 214 dpm/mm2) were measured. The mRNAs for both of these genes were more broadly expressed across the tissue sections. The hybridization pattern for VEGF mRNAs was consistent with hypoxia-induced VEGF mRNA steady-state levels and supports the hypothesis that oxidative stress regulates VEGF expression in breast tumors.</description><identifier>ISSN: 0014-4800</identifier><identifier>EISSN: 1096-0945</identifier><identifier>DOI: 10.1006/exmp.1997.2208</identifier><identifier>PMID: 9203508</identifier><identifier>CODEN: EXMPA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Biological and medical sciences ; Biomarkers, Tumor ; Breast Neoplasms - chemistry ; Breast Neoplasms - pathology ; DNA Primers - chemistry ; Endothelial Growth Factors - analysis ; Endothelial Growth Factors - genetics ; Female ; Glyceraldehyde-3-Phosphate Dehydrogenases - analysis ; Gynecology. Andrology. Obstetrics ; Humans ; In Situ Hybridization ; Lymph Nodes - chemistry ; Lymph Nodes - pathology ; Lymphatic Metastasis ; Lymphokines - analysis ; Lymphokines - genetics ; Mammary gland diseases ; Medical sciences ; Proto-Oncogene Proteins c-myc - analysis ; RNA, Messenger - analysis ; Tumors ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors</subject><ispartof>Experimental and molecular pathology, 1997-02, Vol.64 (1), p.41-51</ispartof><rights>1997 Academic Press</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-49bdfa1f3ac17c8ca8bf9634124dff8d06b0fa8a0392ae91d0d3f6920800b2d23</citedby><cites>FETCH-LOGICAL-c463t-49bdfa1f3ac17c8ca8bf9634124dff8d06b0fa8a0392ae91d0d3f6920800b2d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014480097922085$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2810051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9203508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wright, Paul S.</creatorcontrib><creatorcontrib>Loudy, David E.</creatorcontrib><creatorcontrib>Cross-Doersen, Doreen E.</creatorcontrib><creatorcontrib>Montgomery, Lauren R.</creatorcontrib><creatorcontrib>Sprinkle-Cavallo, Jean</creatorcontrib><creatorcontrib>Miller, Jerry A.</creatorcontrib><creatorcontrib>Distler, Carole M.</creatorcontrib><creatorcontrib>Lower, Elyse E.</creatorcontrib><creatorcontrib>Woessner, Richard D.</creatorcontrib><title>Quantitation of Vascular Endothelial Growth Factor mRNA Levels in Human Breast Tumors and Metastatic Lymph Nodes</title><title>Experimental and molecular pathology</title><addtitle>Exp Mol Pathol</addtitle><description>In situhybridization analysis provides a means to qualitatively study the heterogeneity of primary tumors and metastases based on the types of genes transcribed. In this study, we have tested some parameters for quantitative analysis ofin situhybridizations with paraffin-embedded human breast tumors and measured mRNA levels for the angiogenic protein, vascular endothelial growth factor (VEGF). VEGF mRNAs were highly tumor specific, with the highest levels near necrotic regions within the tissues (0.1 to 2.7 dpm/mm2). Normal cells within the tissue sections did not have detectable levels of VEGF mRNA. For comparison, tumor levels of c-myc(4 to 46 dpm/mm2) and glyceraldehyde-3-phosphate dehydrogenase mRNAs (48 to 214 dpm/mm2) were measured. The mRNAs for both of these genes were more broadly expressed across the tissue sections. The hybridization pattern for VEGF mRNAs was consistent with hypoxia-induced VEGF mRNA steady-state levels and supports the hypothesis that oxidative stress regulates VEGF expression in breast tumors.</description><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor</subject><subject>Breast Neoplasms - chemistry</subject><subject>Breast Neoplasms - pathology</subject><subject>DNA Primers - chemistry</subject><subject>Endothelial Growth Factors - analysis</subject><subject>Endothelial Growth Factors - genetics</subject><subject>Female</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - analysis</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Lymph Nodes - chemistry</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Lymphokines - analysis</subject><subject>Lymphokines - genetics</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Proto-Oncogene Proteins c-myc - analysis</subject><subject>RNA, Messenger - analysis</subject><subject>Tumors</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><issn>0014-4800</issn><issn>1096-0945</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi0EKkvhyg3JB8QtyzjJZu1jqfqBtBSBCldrYo-1RkkcbKfQf49Xu-qN00gzz7yaeRh7K2AtALqP9Hec10Kp7bquQT5jKwGqq0C1m-dsBSDaqpUAL9mrlH4BgAJRn7EzVUOzAbli87cFp-wzZh8mHhz_icksA0Z-NdmQ9zR4HPhNDH_ynl-jySHy8fvdBd_RAw2J-4nfLiNO_FMkTJnfL2OIieNk-RfKpVOCDd89jvOe3wVL6TV74XBI9OZUz9mP66v7y9tq9_Xm8-XFrjJt1-SqVb11KFyDRmyNNCh7p7qmFXVrnZMWuh4cSoRG1UhKWLCN68pb5dm-tnVzzj4cc-cYfi-Ush59MjQMOFFYkt6qok9CW8D1ETQxpBTJ6Tn6EeOjFqAPivVBsT4o1gfFZeHdKXnpR7JP-Mlpmb8_zYtKHFzEyfj0hNWyhG5EweQRKxrpwVPUyXiaDFkfyWRtg__fBf8ASZuYew</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Wright, Paul S.</creator><creator>Loudy, David E.</creator><creator>Cross-Doersen, Doreen E.</creator><creator>Montgomery, Lauren R.</creator><creator>Sprinkle-Cavallo, Jean</creator><creator>Miller, Jerry A.</creator><creator>Distler, Carole M.</creator><creator>Lower, Elyse E.</creator><creator>Woessner, Richard D.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>Quantitation of Vascular Endothelial Growth Factor mRNA Levels in Human Breast Tumors and Metastatic Lymph Nodes</title><author>Wright, Paul S. ; Loudy, David E. ; Cross-Doersen, Doreen E. ; Montgomery, Lauren R. ; Sprinkle-Cavallo, Jean ; Miller, Jerry A. ; Distler, Carole M. ; Lower, Elyse E. ; Woessner, Richard D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-49bdfa1f3ac17c8ca8bf9634124dff8d06b0fa8a0392ae91d0d3f6920800b2d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor</topic><topic>Breast Neoplasms - chemistry</topic><topic>Breast Neoplasms - pathology</topic><topic>DNA Primers - chemistry</topic><topic>Endothelial Growth Factors - analysis</topic><topic>Endothelial Growth Factors - genetics</topic><topic>Female</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - analysis</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Lymph Nodes - chemistry</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphatic Metastasis</topic><topic>Lymphokines - analysis</topic><topic>Lymphokines - genetics</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Proto-Oncogene Proteins c-myc - analysis</topic><topic>RNA, Messenger - analysis</topic><topic>Tumors</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wright, Paul S.</creatorcontrib><creatorcontrib>Loudy, David E.</creatorcontrib><creatorcontrib>Cross-Doersen, Doreen E.</creatorcontrib><creatorcontrib>Montgomery, Lauren R.</creatorcontrib><creatorcontrib>Sprinkle-Cavallo, Jean</creatorcontrib><creatorcontrib>Miller, Jerry A.</creatorcontrib><creatorcontrib>Distler, Carole M.</creatorcontrib><creatorcontrib>Lower, Elyse E.</creatorcontrib><creatorcontrib>Woessner, Richard D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental and molecular pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wright, Paul S.</au><au>Loudy, David E.</au><au>Cross-Doersen, Doreen E.</au><au>Montgomery, Lauren R.</au><au>Sprinkle-Cavallo, Jean</au><au>Miller, Jerry A.</au><au>Distler, Carole M.</au><au>Lower, Elyse E.</au><au>Woessner, Richard D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitation of Vascular Endothelial Growth Factor mRNA Levels in Human Breast Tumors and Metastatic Lymph Nodes</atitle><jtitle>Experimental and molecular pathology</jtitle><addtitle>Exp Mol Pathol</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>64</volume><issue>1</issue><spage>41</spage><epage>51</epage><pages>41-51</pages><issn>0014-4800</issn><eissn>1096-0945</eissn><coden>EXMPA6</coden><abstract>In situhybridization analysis provides a means to qualitatively study the heterogeneity of primary tumors and metastases based on the types of genes transcribed. In this study, we have tested some parameters for quantitative analysis ofin situhybridizations with paraffin-embedded human breast tumors and measured mRNA levels for the angiogenic protein, vascular endothelial growth factor (VEGF). VEGF mRNAs were highly tumor specific, with the highest levels near necrotic regions within the tissues (0.1 to 2.7 dpm/mm2). Normal cells within the tissue sections did not have detectable levels of VEGF mRNA. For comparison, tumor levels of c-myc(4 to 46 dpm/mm2) and glyceraldehyde-3-phosphate dehydrogenase mRNAs (48 to 214 dpm/mm2) were measured. The mRNAs for both of these genes were more broadly expressed across the tissue sections. The hybridization pattern for VEGF mRNAs was consistent with hypoxia-induced VEGF mRNA steady-state levels and supports the hypothesis that oxidative stress regulates VEGF expression in breast tumors.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>9203508</pmid><doi>10.1006/exmp.1997.2208</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental and molecular pathology, 1997-02, Vol.64 (1), p.41-51 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biomarkers, Tumor Breast Neoplasms - chemistry Breast Neoplasms - pathology DNA Primers - chemistry Endothelial Growth Factors - analysis Endothelial Growth Factors - genetics Female Glyceraldehyde-3-Phosphate Dehydrogenases - analysis Gynecology. Andrology. Obstetrics Humans In Situ Hybridization Lymph Nodes - chemistry Lymph Nodes - pathology Lymphatic Metastasis Lymphokines - analysis Lymphokines - genetics Mammary gland diseases Medical sciences Proto-Oncogene Proteins c-myc - analysis RNA, Messenger - analysis Tumors Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| title | Quantitation of Vascular Endothelial Growth Factor mRNA Levels in Human Breast Tumors and Metastatic Lymph Nodes |
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