Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces

A procedure for covalent immobilization of functional proteins on silica substrates was developed using thiol-terminal silanes and heterobifunctional crosslinkers. Using this procedure, a high density of functional antibodies was bound to glass cover slips and silica fibers. The amount of anti-IgG a...

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Veröffentlicht in:	Analytical biochemistry 1989-05, Vol.178 (2), p.408-413
Hauptverfasser:	Bhatia, Suresh K., Shriver-Lake, Lisa C., Prior, Kimberly J., Georger, Jacque H., Calvert, Jeffrey M., Bredehorst, Reinhard, Ligler, Frances S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Antigen-Antibody Reactions Biological and medical sciences Biotechnology Cross-Linking Reagents Fundamental and applied biological sciences. Psychology Fundamental immunology Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies Molecular immunology Silicon Dioxide Sulfhydryl Compounds Techniques
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