Potato cysteine proteinase inhibitor gene family: molecular cloning, characterisation and immunocytochemical localisation studies
Potato cysteine proteinase inhibitors (PCPIs) represent a distinct group of proteins as they show no homology to any other known cysteine proteinase inhibitor superfamilies, but they all belong to the Kunitz-type soybean trypsin inhibitor family. cDNA clones for five PCPIs have been isolated and seq...
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| Veröffentlicht in: | Plant molecular biology 1997-05, Vol.34 (2), p.317-323 |
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| container_title | Plant molecular biology |
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| creator | Gruden, K. (Jozef Stefan Inst., Ljubljana (Slovenia). Dept. of Biochemistry and Molecular Biology) Strukelj, B Ravnikar, M Poljsak-Prijatelj, M Mavric, I Brzin, J Pungercar, J Kregar, I |
| description | Potato cysteine proteinase inhibitors (PCPIs) represent a distinct group of proteins as they show no homology to any other known cysteine proteinase inhibitor superfamilies, but they all belong to the Kunitz-type soybean trypsin inhibitor family. cDNA clones for five PCPIs have been isolated and sequenced. Amino acid substitutions occurring in the limited regions forming loops on the surface of these proteins suggest a further classification of PCPIs into three subgroups. Accumulation of PCPI was observed in vacuoles of stems after treatment with jasmonic acid (JA) using immunocytochemical localisation, implying that these inhibitors are part of a potato defence mechanism against insects and pathogens. Genomic DNA analysis show that PCPIs form a multigene family and suggest that their genes do not possess any introns. |
| doi_str_mv | 10.1023/A:1005853026333 |
| format | Article |
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(Jozef Stefan Inst., Ljubljana (Slovenia). Dept. of Biochemistry and Molecular Biology) ; Strukelj, B ; Ravnikar, M ; Poljsak-Prijatelj, M ; Mavric, I ; Brzin, J ; Pungercar, J ; Kregar, I</creator><creatorcontrib>Gruden, K. (Jozef Stefan Inst., Ljubljana (Slovenia). Dept. of Biochemistry and Molecular Biology) ; Strukelj, B ; Ravnikar, M ; Poljsak-Prijatelj, M ; Mavric, I ; Brzin, J ; Pungercar, J ; Kregar, I</creatorcontrib><description>Potato cysteine proteinase inhibitors (PCPIs) represent a distinct group of proteins as they show no homology to any other known cysteine proteinase inhibitor superfamilies, but they all belong to the Kunitz-type soybean trypsin inhibitor family. cDNA clones for five PCPIs have been isolated and sequenced. Amino acid substitutions occurring in the limited regions forming loops on the surface of these proteins suggest a further classification of PCPIs into three subgroups. Accumulation of PCPI was observed in vacuoles of stems after treatment with jasmonic acid (JA) using immunocytochemical localisation, implying that these inhibitors are part of a potato defence mechanism against insects and pathogens. Genomic DNA analysis show that PCPIs form a multigene family and suggest that their genes do not possess any introns.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1005853026333</identifier><identifier>PMID: 9207847</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Amino Acid Sequence ; Amino acids ; CISTEINA ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cloning ; Cloning, Molecular ; CYSTEINE ; Cysteine Proteinase Inhibitors - chemistry ; Cysteine Proteinase Inhibitors - genetics ; Cysteine Proteinase Inhibitors - metabolism ; GENE ; Gene Expression Regulation, Plant ; GENE LOCATION ; GENES ; Genes, Plant ; Genome, Plant ; Immunohistochemistry ; IMMUNOLOGIE ; IMMUNOLOGY ; INMUNOLOGIA ; Intracellular Fluid - enzymology ; LOCALISATION DE GENE ; LOCALIZACION DE GENES ; MOLECULAR CLONING ; Molecular Sequence Data ; Multigene Family ; Potatoes ; PROTEASAS ; PROTEASE ; PROTEASES ; Proteinase inhibitors ; Proteins ; Sequence Analysis, DNA ; SOLANUM TUBEROSUM ; Solanum tuberosum - chemistry ; Solanum tuberosum - enzymology ; Solanum tuberosum - genetics ; Soybeans</subject><ispartof>Plant molecular biology, 1997-05, Vol.34 (2), p.317-323</ispartof><rights>Kluwer Academic Publishers 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-a269aac75040cdf19defcfb4b9469760a03a1d9cb44891a835e89a7f214919e93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9207847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gruden, K. (Jozef Stefan Inst., Ljubljana (Slovenia). Dept. of Biochemistry and Molecular Biology)</creatorcontrib><creatorcontrib>Strukelj, B</creatorcontrib><creatorcontrib>Ravnikar, M</creatorcontrib><creatorcontrib>Poljsak-Prijatelj, M</creatorcontrib><creatorcontrib>Mavric, I</creatorcontrib><creatorcontrib>Brzin, J</creatorcontrib><creatorcontrib>Pungercar, J</creatorcontrib><creatorcontrib>Kregar, I</creatorcontrib><title>Potato cysteine proteinase inhibitor gene family: molecular cloning, characterisation and immunocytochemical localisation studies</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Potato cysteine proteinase inhibitors (PCPIs) represent a distinct group of proteins as they show no homology to any other known cysteine proteinase inhibitor superfamilies, but they all belong to the Kunitz-type soybean trypsin inhibitor family. cDNA clones for five PCPIs have been isolated and sequenced. Amino acid substitutions occurring in the limited regions forming loops on the surface of these proteins suggest a further classification of PCPIs into three subgroups. Accumulation of PCPI was observed in vacuoles of stems after treatment with jasmonic acid (JA) using immunocytochemical localisation, implying that these inhibitors are part of a potato defence mechanism against insects and pathogens. Genomic DNA analysis show that PCPIs form a multigene family and suggest that their genes do not possess any introns.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>CISTEINA</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>CYSTEINE</subject><subject>Cysteine Proteinase Inhibitors - chemistry</subject><subject>Cysteine Proteinase Inhibitors - genetics</subject><subject>Cysteine Proteinase Inhibitors - metabolism</subject><subject>GENE</subject><subject>Gene Expression Regulation, Plant</subject><subject>GENE LOCATION</subject><subject>GENES</subject><subject>Genes, Plant</subject><subject>Genome, Plant</subject><subject>Immunohistochemistry</subject><subject>IMMUNOLOGIE</subject><subject>IMMUNOLOGY</subject><subject>INMUNOLOGIA</subject><subject>Intracellular Fluid - enzymology</subject><subject>LOCALISATION DE GENE</subject><subject>LOCALIZACION DE GENES</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Potatoes</subject><subject>PROTEASAS</subject><subject>PROTEASE</subject><subject>PROTEASES</subject><subject>Proteinase inhibitors</subject><subject>Proteins</subject><subject>Sequence Analysis, DNA</subject><subject>SOLANUM TUBEROSUM</subject><subject>Solanum tuberosum - chemistry</subject><subject>Solanum tuberosum - enzymology</subject><subject>Solanum tuberosum - genetics</subject><subject>Soybeans</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkE1LxDAQhoMouq6ePQnBgyerkyZtGm8ifsGiHvRcpmm6G0kTbdLDHv3nVlwRvMwMvA_DzEPIEYNzBjm_uLpkAEVVcMhLzvkWmbFC8qyAvNomM2ClzIRg-R7Zj_ENYIJ5uUt2VQ6yEnJGPp9DwhSoXsdkrDf0fQjfA0ZDrV_ZxqYw0KWZkg5769aXtA_O6NHhQLUL3vrlGdUrHFAnM9iIyQZP0bfU9v3og16noFemtxoddWGqv0xMY2tNPCA7HbpoDjd9Tl5vb16u77PF093D9dUi0xxYyjAvFaKWBQjQbcdUazrdNaJRolSyBASOrFW6EaJSDCtemEqh7HImFFNG8Tk5_dk7ffgxmpjq3kZtnENvwhhrqUCVHMQEnvwD38I4-Om2WkomSmCT6Dk53kBj05u2fh9sj8O63oj9yzsMNS4nMfXjgilVAYCUgn8BPoyF5Q</recordid><startdate>199705</startdate><enddate>199705</enddate><creator>Gruden, K. (Jozef Stefan Inst., Ljubljana (Slovenia). Dept. of Biochemistry and Molecular Biology)</creator><creator>Strukelj, B</creator><creator>Ravnikar, M</creator><creator>Poljsak-Prijatelj, M</creator><creator>Mavric, I</creator><creator>Brzin, J</creator><creator>Pungercar, J</creator><creator>Kregar, I</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199705</creationdate><title>Potato cysteine proteinase inhibitor gene family: molecular cloning, characterisation and immunocytochemical localisation studies</title><author>Gruden, K. 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(Jozef Stefan Inst., Ljubljana (Slovenia). Dept. of Biochemistry and Molecular Biology)</au><au>Strukelj, B</au><au>Ravnikar, M</au><au>Poljsak-Prijatelj, M</au><au>Mavric, I</au><au>Brzin, J</au><au>Pungercar, J</au><au>Kregar, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potato cysteine proteinase inhibitor gene family: molecular cloning, characterisation and immunocytochemical localisation studies</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1997-05</date><risdate>1997</risdate><volume>34</volume><issue>2</issue><spage>317</spage><epage>323</epage><pages>317-323</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Potato cysteine proteinase inhibitors (PCPIs) represent a distinct group of proteins as they show no homology to any other known cysteine proteinase inhibitor superfamilies, but they all belong to the Kunitz-type soybean trypsin inhibitor family. cDNA clones for five PCPIs have been isolated and sequenced. Amino acid substitutions occurring in the limited regions forming loops on the surface of these proteins suggest a further classification of PCPIs into three subgroups. Accumulation of PCPI was observed in vacuoles of stems after treatment with jasmonic acid (JA) using immunocytochemical localisation, implying that these inhibitors are part of a potato defence mechanism against insects and pathogens. Genomic DNA analysis show that PCPIs form a multigene family and suggest that their genes do not possess any introns.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>9207847</pmid><doi>10.1023/A:1005853026333</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1997-05, Vol.34 (2), p.317-323 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| subjects | Amino Acid Sequence Amino acids CISTEINA CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning Cloning, Molecular CYSTEINE Cysteine Proteinase Inhibitors - chemistry Cysteine Proteinase Inhibitors - genetics Cysteine Proteinase Inhibitors - metabolism GENE Gene Expression Regulation, Plant GENE LOCATION GENES Genes, Plant Genome, Plant Immunohistochemistry IMMUNOLOGIE IMMUNOLOGY INMUNOLOGIA Intracellular Fluid - enzymology LOCALISATION DE GENE LOCALIZACION DE GENES MOLECULAR CLONING Molecular Sequence Data Multigene Family Potatoes PROTEASAS PROTEASE PROTEASES Proteinase inhibitors Proteins Sequence Analysis, DNA SOLANUM TUBEROSUM Solanum tuberosum - chemistry Solanum tuberosum - enzymology Solanum tuberosum - genetics Soybeans |
| title | Potato cysteine proteinase inhibitor gene family: molecular cloning, characterisation and immunocytochemical localisation studies |
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