Detection of oxygen radicals during reperfusion of intestinal cells in vitro
The nitroxide OXANO. (2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl) which in its reduced form, OXANOH (2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine), is capable of reacting with short-lived radicals, forming a secondary stable radical, was used for ESR-detection of radical production in isolated cell...
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Veröffentlicht in: | Free radical biology & medicine 1989, Vol.6 (3), p.251-259 |
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creator | NILSSON, U. A OLSSON, L.-I GHOR, H MOLDEUS, P BYLUND-FELLENIUS, A.-C |
description | The nitroxide OXANO. (2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl) which in its reduced form, OXANOH (2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine), is capable of reacting with short-lived radicals, forming a secondary stable radical, was used for ESR-detection of radical production in isolated cells. The properties of OXANO. and OXANOH in terms of stability in cellular and subcellular systems, membrane permeability and effects on cellular viability were evaluated. Ischemia and reperfusion was simulated in vitro in a preparation of cells from rat intestinal mucosa by incubation at high density (4 X 10(8) cells/ml) under an atmosphere of nitrogen for 25 min and resuspended with fresh oxygenated buffer containing 5 mM OXANOH. A significant increase in radical formation during the 15 min reperfusion period studied was obtained in cells exposed to ischemia compared to control cells incubated at normal density under an atmosphere of oxygen. The addition of 5 microM of the scavenging enzyme superoxide dismutase reduced the radical formation by 50%. The time sequence of the superoxide formation was calculated as the difference in radical production in the presence and absence of superoxide dismutase. |
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A ; OLSSON, L.-I ; GHOR, H ; MOLDEUS, P ; BYLUND-FELLENIUS, A.-C</creator><creatorcontrib>NILSSON, U. A ; OLSSON, L.-I ; GHOR, H ; MOLDEUS, P ; BYLUND-FELLENIUS, A.-C</creatorcontrib><description>The nitroxide OXANO. (2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl) which in its reduced form, OXANOH (2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine), is capable of reacting with short-lived radicals, forming a secondary stable radical, was used for ESR-detection of radical production in isolated cells. The properties of OXANO. and OXANOH in terms of stability in cellular and subcellular systems, membrane permeability and effects on cellular viability were evaluated. Ischemia and reperfusion was simulated in vitro in a preparation of cells from rat intestinal mucosa by incubation at high density (4 X 10(8) cells/ml) under an atmosphere of nitrogen for 25 min and resuspended with fresh oxygenated buffer containing 5 mM OXANOH. A significant increase in radical formation during the 15 min reperfusion period studied was obtained in cells exposed to ischemia compared to control cells incubated at normal density under an atmosphere of oxygen. The addition of 5 microM of the scavenging enzyme superoxide dismutase reduced the radical formation by 50%. The time sequence of the superoxide formation was calculated as the difference in radical production in the presence and absence of superoxide dismutase.</description><identifier>ISSN: 0891-5849</identifier><identifier>EISSN: 1873-4596</identifier><identifier>PMID: 2545549</identifier><identifier>CODEN: FRBMEH</identifier><language>eng</language><publisher>New York, NY: Elsevier Science</publisher><subject>Animals ; Biological and medical sciences ; Cell Membrane - drug effects ; Cell Membrane Permeability - drug effects ; Cell Survival - drug effects ; Cytochrome P-450 Enzyme System - metabolism ; Drug Stability ; Electron Spin Resonance Spectroscopy ; Free Radicals ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glutathione - metabolism ; In Vitro Techniques ; Intestinal Mucosa - blood supply ; Ischemia - metabolism ; Male ; Metyrapone - pharmacology ; Microsomes, Liver - metabolism ; Nitrogen - administration & dosage ; Oxazoles - metabolism ; Oxazoles - pharmacology ; Oxidation-Reduction ; Oxygen - administration & dosage ; Rats ; Rats, Inbred Strains ; Spin Labels ; Superoxide Dismutase - pharmacology ; Superoxides - metabolism ; Vertebrates: digestive system</subject><ispartof>Free radical biology & medicine, 1989, Vol.6 (3), p.251-259</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19489994$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2545549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NILSSON, U. A</creatorcontrib><creatorcontrib>OLSSON, L.-I</creatorcontrib><creatorcontrib>GHOR, H</creatorcontrib><creatorcontrib>MOLDEUS, P</creatorcontrib><creatorcontrib>BYLUND-FELLENIUS, A.-C</creatorcontrib><title>Detection of oxygen radicals during reperfusion of intestinal cells in vitro</title><title>Free radical biology & medicine</title><addtitle>Free Radic Biol Med</addtitle><description>The nitroxide OXANO. (2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl) which in its reduced form, OXANOH (2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine), is capable of reacting with short-lived radicals, forming a secondary stable radical, was used for ESR-detection of radical production in isolated cells. The properties of OXANO. and OXANOH in terms of stability in cellular and subcellular systems, membrane permeability and effects on cellular viability were evaluated. Ischemia and reperfusion was simulated in vitro in a preparation of cells from rat intestinal mucosa by incubation at high density (4 X 10(8) cells/ml) under an atmosphere of nitrogen for 25 min and resuspended with fresh oxygenated buffer containing 5 mM OXANOH. A significant increase in radical formation during the 15 min reperfusion period studied was obtained in cells exposed to ischemia compared to control cells incubated at normal density under an atmosphere of oxygen. The addition of 5 microM of the scavenging enzyme superoxide dismutase reduced the radical formation by 50%. The time sequence of the superoxide formation was calculated as the difference in radical production in the presence and absence of superoxide dismutase.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Drug Stability</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Free Radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glutathione - metabolism</subject><subject>In Vitro Techniques</subject><subject>Intestinal Mucosa - blood supply</subject><subject>Ischemia - metabolism</subject><subject>Male</subject><subject>Metyrapone - pharmacology</subject><subject>Microsomes, Liver - metabolism</subject><subject>Nitrogen - administration & dosage</subject><subject>Oxazoles - metabolism</subject><subject>Oxazoles - pharmacology</subject><subject>Oxidation-Reduction</subject><subject>Oxygen - administration & dosage</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Spin Labels</subject><subject>Superoxide Dismutase - pharmacology</subject><subject>Superoxides - metabolism</subject><subject>Vertebrates: digestive system</subject><issn>0891-5849</issn><issn>1873-4596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j09LxDAUxIMo67r6EYRc9FZIN0n73lHWv7DgZe8lTV6WyG5ak1bcb2_F4mkO85th5owtS6hloTRW52wpAMtCg8JLdpXzhxBCaQkLtlhrpbXCJds-0kB2CF3knefd92lPkSfjgjWHzN2YQtzzRD0lP-aZCnGgPIRoDtzSYcJC5F9hSN01u_BTjG5mXbHd89Nu81ps31_eNg_bogeJBUqAmlpphJJOG4FOOlIkWiG9BVBrJOOcb8n5EoQR0JKsamc1gLUOpFyx-7_aPnWf4zSlOYb8u8RE6sbc1ChQVLKawNsZHNsjuaZP4WjSqZnfT_7d7Js8_fXJRBvyP1aiAkRU8gcmlWVt</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>NILSSON, U. A</creator><creator>OLSSON, L.-I</creator><creator>GHOR, H</creator><creator>MOLDEUS, P</creator><creator>BYLUND-FELLENIUS, A.-C</creator><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Detection of oxygen radicals during reperfusion of intestinal cells in vitro</title><author>NILSSON, U. A ; OLSSON, L.-I ; GHOR, H ; MOLDEUS, P ; BYLUND-FELLENIUS, A.-C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p839-93887eb3a043d5a09d3de4e0b03fc88429eaddfbedf180a08be367dc588ccd833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Drug Stability</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Free Radicals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Glutathione - metabolism</topic><topic>In Vitro Techniques</topic><topic>Intestinal Mucosa - blood supply</topic><topic>Ischemia - metabolism</topic><topic>Male</topic><topic>Metyrapone - pharmacology</topic><topic>Microsomes, Liver - metabolism</topic><topic>Nitrogen - administration & dosage</topic><topic>Oxazoles - metabolism</topic><topic>Oxazoles - pharmacology</topic><topic>Oxidation-Reduction</topic><topic>Oxygen - administration & dosage</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Spin Labels</topic><topic>Superoxide Dismutase - pharmacology</topic><topic>Superoxides - metabolism</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NILSSON, U. A</creatorcontrib><creatorcontrib>OLSSON, L.-I</creatorcontrib><creatorcontrib>GHOR, H</creatorcontrib><creatorcontrib>MOLDEUS, P</creatorcontrib><creatorcontrib>BYLUND-FELLENIUS, A.-C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical biology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NILSSON, U. A</au><au>OLSSON, L.-I</au><au>GHOR, H</au><au>MOLDEUS, P</au><au>BYLUND-FELLENIUS, A.-C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of oxygen radicals during reperfusion of intestinal cells in vitro</atitle><jtitle>Free radical biology & medicine</jtitle><addtitle>Free Radic Biol Med</addtitle><date>1989</date><risdate>1989</risdate><volume>6</volume><issue>3</issue><spage>251</spage><epage>259</epage><pages>251-259</pages><issn>0891-5849</issn><eissn>1873-4596</eissn><coden>FRBMEH</coden><abstract>The nitroxide OXANO. (2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl) which in its reduced form, OXANOH (2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine), is capable of reacting with short-lived radicals, forming a secondary stable radical, was used for ESR-detection of radical production in isolated cells. The properties of OXANO. and OXANOH in terms of stability in cellular and subcellular systems, membrane permeability and effects on cellular viability were evaluated. Ischemia and reperfusion was simulated in vitro in a preparation of cells from rat intestinal mucosa by incubation at high density (4 X 10(8) cells/ml) under an atmosphere of nitrogen for 25 min and resuspended with fresh oxygenated buffer containing 5 mM OXANOH. A significant increase in radical formation during the 15 min reperfusion period studied was obtained in cells exposed to ischemia compared to control cells incubated at normal density under an atmosphere of oxygen. The addition of 5 microM of the scavenging enzyme superoxide dismutase reduced the radical formation by 50%. The time sequence of the superoxide formation was calculated as the difference in radical production in the presence and absence of superoxide dismutase.</abstract><cop>New York, NY</cop><pub>Elsevier Science</pub><pmid>2545549</pmid><tpages>9</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Cell Membrane - drug effects Cell Membrane Permeability - drug effects Cell Survival - drug effects Cytochrome P-450 Enzyme System - metabolism Drug Stability Electron Spin Resonance Spectroscopy Free Radicals Fundamental and applied biological sciences. Psychology General aspects Glutathione - metabolism In Vitro Techniques Intestinal Mucosa - blood supply Ischemia - metabolism Male Metyrapone - pharmacology Microsomes, Liver - metabolism Nitrogen - administration & dosage Oxazoles - metabolism Oxazoles - pharmacology Oxidation-Reduction Oxygen - administration & dosage Rats Rats, Inbred Strains Spin Labels Superoxide Dismutase - pharmacology Superoxides - metabolism Vertebrates: digestive system |
title | Detection of oxygen radicals during reperfusion of intestinal cells in vitro |
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