Serotonin induces inward potassium and calcium currents in rat cortical astrocytes
Ca 2+ imaging and patch-clamp techniques were used to study the effects of serotonin (5-HT) on ionic conductances in rat cortical astrocytes. 1 and 10 μM serotonin caused a transient increase in intracellular calcium (Ca 1) levels in fura-2AM-loaded cultured astrocytes and in astrocytes acutely isol...
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| Veröffentlicht in: | Brain research 1997-05, Vol.758 (1), p.69-82 |
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| creator | Jalonen, T.O. Margraf, R.R. Wielt, D.B. Charniga, C.J. Linne, M.-L. Kimelberg, H.K. |
| description | Ca
2+ imaging and patch-clamp techniques were used to study the effects of serotonin (5-HT) on ionic conductances in rat cortical astrocytes. 1 and 10 μM serotonin caused a transient increase in intracellular calcium (Ca
1) levels in fura-2AM-loaded cultured astrocytes and in astrocytes acutely isolated and then cultured in horse serum-containing medium for over 24 h. However, the acutely isolated (less than 6 h from isolation) astrocytes, as well as acutely isolated astrocytes cultured in serum-free media, failed to respond to 5-HT by changes in Ca
1. Coinciding with the changes in Ca
1 levels, inward currents were activated by 10 μM 5-HT in cultured, but not in acutely isolated astrocytes. Two separate types of serotonin-induced, small-conductance inward single-channel currents were found. First, in both Ca
2+-containing and Ca
2+-free media serotonin transiently activated a small-conductance apamin-sensitive channel. Apamin is a specific blocker of the small-conductance Ca
2+-activated K
+ channel (sK
Ca). When cells were pre-treated with phospholipase C inhibitor U73122 no 5-HT-induced sK
Ca channel openings were seen, indicating that this channel was activated by Ca
2+ released from intracellular stores via IP
3. A second type of small inward channel activated later, but only in the presence of external Ca
2+. It was inhibited by the L-type Ca
2+ channel blockers, nimodipine and nifedipine. Both types of channel activity were inhibited by ketanserin, indicating activation of the 5-HT
2A receptor. |
| doi_str_mv | 10.1016/S0006-8993(97)00163-7 |
| format | Article |
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2+ imaging and patch-clamp techniques were used to study the effects of serotonin (5-HT) on ionic conductances in rat cortical astrocytes. 1 and 10 μM serotonin caused a transient increase in intracellular calcium (Ca
1) levels in fura-2AM-loaded cultured astrocytes and in astrocytes acutely isolated and then cultured in horse serum-containing medium for over 24 h. However, the acutely isolated (less than 6 h from isolation) astrocytes, as well as acutely isolated astrocytes cultured in serum-free media, failed to respond to 5-HT by changes in Ca
1. Coinciding with the changes in Ca
1 levels, inward currents were activated by 10 μM 5-HT in cultured, but not in acutely isolated astrocytes. Two separate types of serotonin-induced, small-conductance inward single-channel currents were found. First, in both Ca
2+-containing and Ca
2+-free media serotonin transiently activated a small-conductance apamin-sensitive channel. Apamin is a specific blocker of the small-conductance Ca
2+-activated K
+ channel (sK
Ca). When cells were pre-treated with phospholipase C inhibitor U73122 no 5-HT-induced sK
Ca channel openings were seen, indicating that this channel was activated by Ca
2+ released from intracellular stores via IP
3. A second type of small inward channel activated later, but only in the presence of external Ca
2+. It was inhibited by the L-type Ca
2+ channel blockers, nimodipine and nifedipine. Both types of channel activity were inhibited by ketanserin, indicating activation of the 5-HT
2A receptor.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(97)00163-7</identifier><identifier>PMID: 9203535</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>5-HT 2A ; Animals ; Astrocytes - drug effects ; Astrocytes - metabolism ; Calcium channel ; Calcium Channels - drug effects ; Cells, Cultured ; Cultured astrocyte ; Electrophysiology ; Intracellular calcium ; Ion Transport - drug effects ; Potassium Channels - drug effects ; Rats ; Rats, Sprague-Dawley ; Serotonin - pharmacology ; sK Ca channel</subject><ispartof>Brain research, 1997-05, Vol.758 (1), p.69-82</ispartof><rights>1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-2567fd30348be0b119a61eafde2254027cb5c72c1f1fbaec648c287958d6081f3</citedby><cites>FETCH-LOGICAL-c443t-2567fd30348be0b119a61eafde2254027cb5c72c1f1fbaec648c287958d6081f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-8993(97)00163-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9203535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jalonen, T.O.</creatorcontrib><creatorcontrib>Margraf, R.R.</creatorcontrib><creatorcontrib>Wielt, D.B.</creatorcontrib><creatorcontrib>Charniga, C.J.</creatorcontrib><creatorcontrib>Linne, M.-L.</creatorcontrib><creatorcontrib>Kimelberg, H.K.</creatorcontrib><title>Serotonin induces inward potassium and calcium currents in rat cortical astrocytes</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Ca
2+ imaging and patch-clamp techniques were used to study the effects of serotonin (5-HT) on ionic conductances in rat cortical astrocytes. 1 and 10 μM serotonin caused a transient increase in intracellular calcium (Ca
1) levels in fura-2AM-loaded cultured astrocytes and in astrocytes acutely isolated and then cultured in horse serum-containing medium for over 24 h. However, the acutely isolated (less than 6 h from isolation) astrocytes, as well as acutely isolated astrocytes cultured in serum-free media, failed to respond to 5-HT by changes in Ca
1. Coinciding with the changes in Ca
1 levels, inward currents were activated by 10 μM 5-HT in cultured, but not in acutely isolated astrocytes. Two separate types of serotonin-induced, small-conductance inward single-channel currents were found. First, in both Ca
2+-containing and Ca
2+-free media serotonin transiently activated a small-conductance apamin-sensitive channel. Apamin is a specific blocker of the small-conductance Ca
2+-activated K
+ channel (sK
Ca). When cells were pre-treated with phospholipase C inhibitor U73122 no 5-HT-induced sK
Ca channel openings were seen, indicating that this channel was activated by Ca
2+ released from intracellular stores via IP
3. A second type of small inward channel activated later, but only in the presence of external Ca
2+. It was inhibited by the L-type Ca
2+ channel blockers, nimodipine and nifedipine. Both types of channel activity were inhibited by ketanserin, indicating activation of the 5-HT
2A receptor.</description><subject>5-HT 2A</subject><subject>Animals</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>Calcium channel</subject><subject>Calcium Channels - drug effects</subject><subject>Cells, Cultured</subject><subject>Cultured astrocyte</subject><subject>Electrophysiology</subject><subject>Intracellular calcium</subject><subject>Ion Transport - drug effects</subject><subject>Potassium Channels - drug effects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Serotonin - pharmacology</subject><subject>sK Ca channel</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1qGzEUhUVJSZy0jxCYVUkX0-pnRj-rEkKaBAyFul0LzdUdULFHjqRJ8Nt3xjbeenUlne_qwEfILaPfGGXy-4pSKmttjLgz6iudnkStPpAF04rXkjf0gixOyBW5zvnfdBXC0EtyaTgVrWgX5PcKUyxxCEMVBj8C5mm-u-SrbSwu5zBuKjf4Ctwa5jOMKeFQZqpKrlQQUwlTWLlcUoRdwfyJfOzdOuPn47whf38-_nl4rpe_nl4e7pc1NI0oNW-l6r2gotEd0o4x4yRD13vkvG0oV9C1oDiwnvWdQ5CNBq6VabWXVLNe3JAvh3-3Kb6OmIvdhAy4XrsB45itMlQrSeVZkLVGT5VqAtsDCCnmnLC32xQ2Lu0so3aWbvfS7WzUGmX30u28d3ssGLsN-tPW0fKU_zjkOOl4C5hshoADoA8JoVgfw5mG_zBVkgs</recordid><startdate>19970530</startdate><enddate>19970530</enddate><creator>Jalonen, T.O.</creator><creator>Margraf, R.R.</creator><creator>Wielt, D.B.</creator><creator>Charniga, C.J.</creator><creator>Linne, M.-L.</creator><creator>Kimelberg, H.K.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19970530</creationdate><title>Serotonin induces inward potassium and calcium currents in rat cortical astrocytes</title><author>Jalonen, T.O. ; Margraf, R.R. ; Wielt, D.B. ; Charniga, C.J. ; Linne, M.-L. ; Kimelberg, H.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-2567fd30348be0b119a61eafde2254027cb5c72c1f1fbaec648c287958d6081f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>5-HT 2A</topic><topic>Animals</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - metabolism</topic><topic>Calcium channel</topic><topic>Calcium Channels - drug effects</topic><topic>Cells, Cultured</topic><topic>Cultured astrocyte</topic><topic>Electrophysiology</topic><topic>Intracellular calcium</topic><topic>Ion Transport - drug effects</topic><topic>Potassium Channels - drug effects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Serotonin - pharmacology</topic><topic>sK Ca channel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jalonen, T.O.</creatorcontrib><creatorcontrib>Margraf, R.R.</creatorcontrib><creatorcontrib>Wielt, D.B.</creatorcontrib><creatorcontrib>Charniga, C.J.</creatorcontrib><creatorcontrib>Linne, M.-L.</creatorcontrib><creatorcontrib>Kimelberg, H.K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jalonen, T.O.</au><au>Margraf, R.R.</au><au>Wielt, D.B.</au><au>Charniga, C.J.</au><au>Linne, M.-L.</au><au>Kimelberg, H.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serotonin induces inward potassium and calcium currents in rat cortical astrocytes</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1997-05-30</date><risdate>1997</risdate><volume>758</volume><issue>1</issue><spage>69</spage><epage>82</epage><pages>69-82</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>Ca
2+ imaging and patch-clamp techniques were used to study the effects of serotonin (5-HT) on ionic conductances in rat cortical astrocytes. 1 and 10 μM serotonin caused a transient increase in intracellular calcium (Ca
1) levels in fura-2AM-loaded cultured astrocytes and in astrocytes acutely isolated and then cultured in horse serum-containing medium for over 24 h. However, the acutely isolated (less than 6 h from isolation) astrocytes, as well as acutely isolated astrocytes cultured in serum-free media, failed to respond to 5-HT by changes in Ca
1. Coinciding with the changes in Ca
1 levels, inward currents were activated by 10 μM 5-HT in cultured, but not in acutely isolated astrocytes. Two separate types of serotonin-induced, small-conductance inward single-channel currents were found. First, in both Ca
2+-containing and Ca
2+-free media serotonin transiently activated a small-conductance apamin-sensitive channel. Apamin is a specific blocker of the small-conductance Ca
2+-activated K
+ channel (sK
Ca). When cells were pre-treated with phospholipase C inhibitor U73122 no 5-HT-induced sK
Ca channel openings were seen, indicating that this channel was activated by Ca
2+ released from intracellular stores via IP
3. A second type of small inward channel activated later, but only in the presence of external Ca
2+. It was inhibited by the L-type Ca
2+ channel blockers, nimodipine and nifedipine. Both types of channel activity were inhibited by ketanserin, indicating activation of the 5-HT
2A receptor.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9203535</pmid><doi>10.1016/S0006-8993(97)00163-7</doi><tpages>14</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 5-HT 2A Animals Astrocytes - drug effects Astrocytes - metabolism Calcium channel Calcium Channels - drug effects Cells, Cultured Cultured astrocyte Electrophysiology Intracellular calcium Ion Transport - drug effects Potassium Channels - drug effects Rats Rats, Sprague-Dawley Serotonin - pharmacology sK Ca channel |
| title | Serotonin induces inward potassium and calcium currents in rat cortical astrocytes |
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