Anaerobic taurine oxidation: a novel reaction by a nitrate-reducing Alcaligenes sp
Faculty for Biology, The University, D-78434 Konstanz, Germany Author for correspondence: Alasdair M. Cook. Tel: + 49 7531 88 4247. Fax: +49 7531 88 2966. e-mail: Alasdair.Cook@uni-konstanz.de ABSTRACT Enrichment cultures were prepared under strictly anoxic conditions in medium representing fresh wa...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 1997-06, Vol.143 (6), p.1919-1924 |
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| container_end_page | 1924 |
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| container_issue | 6 |
| container_start_page | 1919 |
| container_title | Microbiology (Society for General Microbiology) |
| container_volume | 143 |
| creator | Denger, Karin Laue, Heike Cook, Alasdair M |
| description | Faculty for Biology, The University, D-78434 Konstanz, Germany
Author for correspondence: Alasdair M. Cook. Tel: + 49 7531 88 4247. Fax: +49 7531 88 2966. e-mail: Alasdair.Cook@uni-konstanz.de
ABSTRACT
Enrichment cultures were prepared under strictly anoxic conditions in medium representing fresh water and containing an organosulfonate as electron donor and carbon source, and nitrate as electron acceptor. The inoculum was from the anaerobic digestor of two communal sewage works. The natural organosulfonates 2-aminoethanesulfonate (taurine), DL -2-amino-3-sulfopropionate (cysteate) and 2-hydroxyethanesulfonate (isethionate) all gave positive enrichments, whereas unsubstituted alkanesulfonates, such as methanesulfonate and arenesulfonates, gave no enrichment. Two representative enrichments were used to obtain pure cultures, and strains NKNTAU (utilizing taurine) and NKNIS (utilizing isethionate) were isolated. Strain NKNTAU was examined in detail. Out of 18 tested organosulfonates, it utilized only one, taurine, and was identified as a novel Alcaligenes sp., a facultatively anaerobic bacterium. Carbon from taurine was converted to cell material and carbon dioxide. The amino group was released as ammonium ion and the sulfonate moiety was recovered as sulfate. Nitrate was reduced to nitrogen gas.
Keywords: desulfonation, taurine, nitrate-reducing bacteria, anaerobic degradation, anaerobic desulfonation |
| doi_str_mv | 10.1099/00221287-143-6-1919 |
| format | Article |
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Author for correspondence: Alasdair M. Cook. Tel: + 49 7531 88 4247. Fax: +49 7531 88 2966. e-mail: Alasdair.Cook@uni-konstanz.de
ABSTRACT
Enrichment cultures were prepared under strictly anoxic conditions in medium representing fresh water and containing an organosulfonate as electron donor and carbon source, and nitrate as electron acceptor. The inoculum was from the anaerobic digestor of two communal sewage works. The natural organosulfonates 2-aminoethanesulfonate (taurine), DL -2-amino-3-sulfopropionate (cysteate) and 2-hydroxyethanesulfonate (isethionate) all gave positive enrichments, whereas unsubstituted alkanesulfonates, such as methanesulfonate and arenesulfonates, gave no enrichment. Two representative enrichments were used to obtain pure cultures, and strains NKNTAU (utilizing taurine) and NKNIS (utilizing isethionate) were isolated. Strain NKNTAU was examined in detail. Out of 18 tested organosulfonates, it utilized only one, taurine, and was identified as a novel Alcaligenes sp., a facultatively anaerobic bacterium. Carbon from taurine was converted to cell material and carbon dioxide. The amino group was released as ammonium ion and the sulfonate moiety was recovered as sulfate. Nitrate was reduced to nitrogen gas.
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Author for correspondence: Alasdair M. Cook. Tel: + 49 7531 88 4247. Fax: +49 7531 88 2966. e-mail: Alasdair.Cook@uni-konstanz.de
ABSTRACT
Enrichment cultures were prepared under strictly anoxic conditions in medium representing fresh water and containing an organosulfonate as electron donor and carbon source, and nitrate as electron acceptor. The inoculum was from the anaerobic digestor of two communal sewage works. The natural organosulfonates 2-aminoethanesulfonate (taurine), DL -2-amino-3-sulfopropionate (cysteate) and 2-hydroxyethanesulfonate (isethionate) all gave positive enrichments, whereas unsubstituted alkanesulfonates, such as methanesulfonate and arenesulfonates, gave no enrichment. Two representative enrichments were used to obtain pure cultures, and strains NKNTAU (utilizing taurine) and NKNIS (utilizing isethionate) were isolated. Strain NKNTAU was examined in detail. Out of 18 tested organosulfonates, it utilized only one, taurine, and was identified as a novel Alcaligenes sp., a facultatively anaerobic bacterium. Carbon from taurine was converted to cell material and carbon dioxide. The amino group was released as ammonium ion and the sulfonate moiety was recovered as sulfate. Nitrate was reduced to nitrogen gas.
Keywords: desulfonation, taurine, nitrate-reducing bacteria, anaerobic degradation, anaerobic desulfonation</description><subject>Alcaligenes</subject><subject>Alcaligenes - growth & development</subject><subject>Alcaligenes - isolation & purification</subject><subject>Alcaligenes - metabolism</subject><subject>Ammonia - metabolism</subject><subject>Anaerobiosis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carbon - metabolism</subject><subject>Electron Transport</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nitrates - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Sulfones - metabolism</subject><subject>Taurine - metabolism</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtrGzEQgEVJcZzHLyiFPYRADmpG0uqVmzF9BAyB0J6FVqu1Vda7jrSbJv8-Wuya3HKRxMw3M9InhL4Q-EZA61sASglVEpOSYYGJJvoTmpNScExBwUk-Mw4YlKSn6CylvwA5CWSGZpoCLYWao8dFZ33sq-CKwY4xdL7oX0Jth9B3d4Utuv7Zt0X01k2RonqdYmGIdvA4-np0oVsXi9bZNqx951ORdhfoc2Pb5C8P-zn68-P77-UvvHr4eb9crLDjwAesZV1BQ4W0jRREMl6CZUoJbTU0Fc-LrpmqiVSWMcmFpJTTjIFifnoHO0fX-7672D-NPg1mG5LzbWs734_JSA2KCi0_BImgosySMsj2oIt9StE3ZhfD1sZXQ8BMys1_5SYrN8JMynPV10P7sdr6-lhzcJzzV4e8TdlTE23nQjpiVBINJc_YzR7bhPXmX4jeZKHb4KbP6fON3buRb1PllKA</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>Denger, Karin</creator><creator>Laue, Heike</creator><creator>Cook, Alasdair M</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19970601</creationdate><title>Anaerobic taurine oxidation: a novel reaction by a nitrate-reducing Alcaligenes sp</title><author>Denger, Karin ; Laue, Heike ; Cook, Alasdair M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-97db0f267af76173540a38869a90fb590f9d38d178a3375672252735083e01463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Alcaligenes</topic><topic>Alcaligenes - growth & development</topic><topic>Alcaligenes - isolation & purification</topic><topic>Alcaligenes - metabolism</topic><topic>Ammonia - metabolism</topic><topic>Anaerobiosis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carbon - metabolism</topic><topic>Electron Transport</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nitrates - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Sulfones - metabolism</topic><topic>Taurine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Denger, Karin</creatorcontrib><creatorcontrib>Laue, Heike</creatorcontrib><creatorcontrib>Cook, Alasdair M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Denger, Karin</au><au>Laue, Heike</au><au>Cook, Alasdair M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anaerobic taurine oxidation: a novel reaction by a nitrate-reducing Alcaligenes sp</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology (Reading)</addtitle><date>1997-06-01</date><risdate>1997</risdate><volume>143</volume><issue>6</issue><spage>1919</spage><epage>1924</epage><pages>1919-1924</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Faculty for Biology, The University, D-78434 Konstanz, Germany
Author for correspondence: Alasdair M. Cook. Tel: + 49 7531 88 4247. Fax: +49 7531 88 2966. e-mail: Alasdair.Cook@uni-konstanz.de
ABSTRACT
Enrichment cultures were prepared under strictly anoxic conditions in medium representing fresh water and containing an organosulfonate as electron donor and carbon source, and nitrate as electron acceptor. The inoculum was from the anaerobic digestor of two communal sewage works. The natural organosulfonates 2-aminoethanesulfonate (taurine), DL -2-amino-3-sulfopropionate (cysteate) and 2-hydroxyethanesulfonate (isethionate) all gave positive enrichments, whereas unsubstituted alkanesulfonates, such as methanesulfonate and arenesulfonates, gave no enrichment. Two representative enrichments were used to obtain pure cultures, and strains NKNTAU (utilizing taurine) and NKNIS (utilizing isethionate) were isolated. Strain NKNTAU was examined in detail. Out of 18 tested organosulfonates, it utilized only one, taurine, and was identified as a novel Alcaligenes sp., a facultatively anaerobic bacterium. Carbon from taurine was converted to cell material and carbon dioxide. The amino group was released as ammonium ion and the sulfonate moiety was recovered as sulfate. Nitrate was reduced to nitrogen gas.
Keywords: desulfonation, taurine, nitrate-reducing bacteria, anaerobic degradation, anaerobic desulfonation</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>9202468</pmid><doi>10.1099/00221287-143-6-1919</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Microbiology (Society for General Microbiology), 1997-06, Vol.143 (6), p.1919-1924 |
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| subjects | Alcaligenes Alcaligenes - growth & development Alcaligenes - isolation & purification Alcaligenes - metabolism Ammonia - metabolism Anaerobiosis Bacteriology Biological and medical sciences Carbon - metabolism Electron Transport Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Molecular Sequence Data Nitrates - metabolism Oxidation-Reduction Sulfones - metabolism Taurine - metabolism |
| title | Anaerobic taurine oxidation: a novel reaction by a nitrate-reducing Alcaligenes sp |
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