Conversion of Big Endothelin-1 to 21-Residue Endothelin-1 Is Essential for Expression of Full Vasoconstrictor Activity: Structure-Activity Relationships of Big Endothelin-1
SUMMARYThe vasoconstrictor activities of porcine big endothelin-1 (big ET-1), a 39-residue intermediate predicted from cDNA sequence analysis, and of its shorter derivative, big ET-1 [1–25], were characterized in vitro by measuring the contraction of porcine coronary artery strips. Synthetic big ET-...
Gespeichert in:
| Veröffentlicht in: | Journal of cardiovascular pharmacology 1989, Vol.13 Suppl 5, p.S5-7 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7 |
|---|---|
| container_issue | |
| container_start_page | S5 |
| container_title | Journal of cardiovascular pharmacology |
| container_volume | 13 Suppl 5 |
| creator | Kimura, Sadao Kasuya, Yoshitoshi Sawamura, Tatsuya Shinmi, Osamu Sugita, Yoshiki Yanagisawa, Masashi Goto, Katsutoshi Masaki, Tomoh |
| description | SUMMARYThe vasoconstrictor activities of porcine big endothelin-1 (big ET-1), a 39-residue intermediate predicted from cDNA sequence analysis, and of its shorter derivative, big ET-1 [1–25], were characterized in vitro by measuring the contraction of porcine coronary artery strips. Synthetic big ET-1 [1–39] and big ET-1 [1–25] induced a slow developing, long-lasting, and strong vasoconstriction as in the case of 21-residue ET-1. However, the contractile molar potencies of big ET-1 [1–39] and big ET-1 [1–25] were approximately 140-and 50-fold lower than that of ET-1, respectively. These results indicate that the conversion of big ET-1 to “mature” ET-1 is essential for the expression of the full vasoconstrictor activity, suggesting the physiological importance of the unusual proteolytic processing catalyzed by the putative “ET converting enzyme.” |
| doi_str_mv | 10.1097/00005344-198900135-00003 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79081145</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79081145</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3003-f4b8231887ad41c239fa71a355275dbe1d0052124cab30c585dc9024619756523</originalsourceid><addsrcrecordid>eNp1kc1uEzEUhS1UVELLIyB5xc7Fv-MZdiVKS6VKSAW6tRyPh7h1xsHX05934iFxSVIJiXpj6Z5zrq3zIYQZPWG00x9pPUpISVjXdpQyocjTSLxCM6aEIJJycYBmlDWUcCmbN-gtwE01SqWbQ3TIpRaC6xn6PU_jnc8Q0ojTgD-Hn3gx9qmsfAwjYbgkzBm58hD6yf8rXQBeAPixBBvxkDJePGyyh_2qsylGfG0huTRCycGVajl1JdyF8vgJfyt5cmXKnuxn-MpHW2oaVmED__vNMXo92Aj-3e4-Qj_OFt_nX8jl1_OL-eklcaJ2QAa5bLlgbattL5njohusZlYoxbXql571tTzOuHR2KahTrepdR7lsWKdVo7g4Qh-2ezc5_Zo8FLMO4HyMdvRpAqM72rJaZTW2W6PLCSD7wWxyWNv8aBg1T6DMHpR5BvV3JGr0_e6Nabn2_XNwR6bqcqvfp1gqoNs43ftsVt7GsjIv8Rd_AO00noo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79081145</pqid></control><display><type>article</type><title>Conversion of Big Endothelin-1 to 21-Residue Endothelin-1 Is Essential for Expression of Full Vasoconstrictor Activity: Structure-Activity Relationships of Big Endothelin-1</title><source>MEDLINE</source><source>Journals@Ovid LWW Legacy Archive</source><source>Journals@Ovid Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Kimura, Sadao ; Kasuya, Yoshitoshi ; Sawamura, Tatsuya ; Shinmi, Osamu ; Sugita, Yoshiki ; Yanagisawa, Masashi ; Goto, Katsutoshi ; Masaki, Tomoh</creator><creatorcontrib>Kimura, Sadao ; Kasuya, Yoshitoshi ; Sawamura, Tatsuya ; Shinmi, Osamu ; Sugita, Yoshiki ; Yanagisawa, Masashi ; Goto, Katsutoshi ; Masaki, Tomoh</creatorcontrib><description>SUMMARYThe vasoconstrictor activities of porcine big endothelin-1 (big ET-1), a 39-residue intermediate predicted from cDNA sequence analysis, and of its shorter derivative, big ET-1 [1–25], were characterized in vitro by measuring the contraction of porcine coronary artery strips. Synthetic big ET-1 [1–39] and big ET-1 [1–25] induced a slow developing, long-lasting, and strong vasoconstriction as in the case of 21-residue ET-1. However, the contractile molar potencies of big ET-1 [1–39] and big ET-1 [1–25] were approximately 140-and 50-fold lower than that of ET-1, respectively. These results indicate that the conversion of big ET-1 to “mature” ET-1 is essential for the expression of the full vasoconstrictor activity, suggesting the physiological importance of the unusual proteolytic processing catalyzed by the putative “ET converting enzyme.”</description><identifier>ISSN: 0160-2446</identifier><identifier>EISSN: 1533-4023</identifier><identifier>DOI: 10.1097/00005344-198900135-00003</identifier><identifier>PMID: 2473327</identifier><language>eng</language><publisher>United States: Lippincott-Raven Publishers</publisher><subject>Animals ; Endothelins ; In Vitro Techniques ; Muscle Contraction - drug effects ; Muscle, Smooth, Vascular - drug effects ; Peptides - analysis ; Peptides - pharmacology ; Structure-Activity Relationship ; Swine ; Vasoconstriction - drug effects</subject><ispartof>Journal of cardiovascular pharmacology, 1989, Vol.13 Suppl 5, p.S5-7</ispartof><rights>Lippincott-Raven Publishers.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf><![CDATA[$$Uhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&PDF=y&D=ovft&AN=00005344-198900135-00003$$EPDF$$P50$$Gwolterskluwer$$H]]></linktopdf><linktohtml>$$Uhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=n&CSC=Y&PAGE=fulltext&D=ovft&AN=00005344-198900135-00003$$EHTML$$P50$$Gwolterskluwer$$H</linktohtml><link.rule.ids>314,780,784,4024,4609,27923,27924,27925,64666,65461</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2473327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kimura, Sadao</creatorcontrib><creatorcontrib>Kasuya, Yoshitoshi</creatorcontrib><creatorcontrib>Sawamura, Tatsuya</creatorcontrib><creatorcontrib>Shinmi, Osamu</creatorcontrib><creatorcontrib>Sugita, Yoshiki</creatorcontrib><creatorcontrib>Yanagisawa, Masashi</creatorcontrib><creatorcontrib>Goto, Katsutoshi</creatorcontrib><creatorcontrib>Masaki, Tomoh</creatorcontrib><title>Conversion of Big Endothelin-1 to 21-Residue Endothelin-1 Is Essential for Expression of Full Vasoconstrictor Activity: Structure-Activity Relationships of Big Endothelin-1</title><title>Journal of cardiovascular pharmacology</title><addtitle>J Cardiovasc Pharmacol</addtitle><description>SUMMARYThe vasoconstrictor activities of porcine big endothelin-1 (big ET-1), a 39-residue intermediate predicted from cDNA sequence analysis, and of its shorter derivative, big ET-1 [1–25], were characterized in vitro by measuring the contraction of porcine coronary artery strips. Synthetic big ET-1 [1–39] and big ET-1 [1–25] induced a slow developing, long-lasting, and strong vasoconstriction as in the case of 21-residue ET-1. However, the contractile molar potencies of big ET-1 [1–39] and big ET-1 [1–25] were approximately 140-and 50-fold lower than that of ET-1, respectively. These results indicate that the conversion of big ET-1 to “mature” ET-1 is essential for the expression of the full vasoconstrictor activity, suggesting the physiological importance of the unusual proteolytic processing catalyzed by the putative “ET converting enzyme.”</description><subject>Animals</subject><subject>Endothelins</subject><subject>In Vitro Techniques</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Peptides - analysis</subject><subject>Peptides - pharmacology</subject><subject>Structure-Activity Relationship</subject><subject>Swine</subject><subject>Vasoconstriction - drug effects</subject><issn>0160-2446</issn><issn>1533-4023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1uEzEUhS1UVELLIyB5xc7Fv-MZdiVKS6VKSAW6tRyPh7h1xsHX05934iFxSVIJiXpj6Z5zrq3zIYQZPWG00x9pPUpISVjXdpQyocjTSLxCM6aEIJJycYBmlDWUcCmbN-gtwE01SqWbQ3TIpRaC6xn6PU_jnc8Q0ojTgD-Hn3gx9qmsfAwjYbgkzBm58hD6yf8rXQBeAPixBBvxkDJePGyyh_2qsylGfG0huTRCycGVajl1JdyF8vgJfyt5cmXKnuxn-MpHW2oaVmED__vNMXo92Aj-3e4-Qj_OFt_nX8jl1_OL-eklcaJ2QAa5bLlgbattL5njohusZlYoxbXql571tTzOuHR2KahTrepdR7lsWKdVo7g4Qh-2ezc5_Zo8FLMO4HyMdvRpAqM72rJaZTW2W6PLCSD7wWxyWNv8aBg1T6DMHpR5BvV3JGr0_e6Nabn2_XNwR6bqcqvfp1gqoNs43ftsVt7GsjIv8Rd_AO00noo</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Kimura, Sadao</creator><creator>Kasuya, Yoshitoshi</creator><creator>Sawamura, Tatsuya</creator><creator>Shinmi, Osamu</creator><creator>Sugita, Yoshiki</creator><creator>Yanagisawa, Masashi</creator><creator>Goto, Katsutoshi</creator><creator>Masaki, Tomoh</creator><general>Lippincott-Raven Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Conversion of Big Endothelin-1 to 21-Residue Endothelin-1 Is Essential for Expression of Full Vasoconstrictor Activity: Structure-Activity Relationships of Big Endothelin-1</title><author>Kimura, Sadao ; Kasuya, Yoshitoshi ; Sawamura, Tatsuya ; Shinmi, Osamu ; Sugita, Yoshiki ; Yanagisawa, Masashi ; Goto, Katsutoshi ; Masaki, Tomoh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3003-f4b8231887ad41c239fa71a355275dbe1d0052124cab30c585dc9024619756523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Endothelins</topic><topic>In Vitro Techniques</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Peptides - analysis</topic><topic>Peptides - pharmacology</topic><topic>Structure-Activity Relationship</topic><topic>Swine</topic><topic>Vasoconstriction - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimura, Sadao</creatorcontrib><creatorcontrib>Kasuya, Yoshitoshi</creatorcontrib><creatorcontrib>Sawamura, Tatsuya</creatorcontrib><creatorcontrib>Shinmi, Osamu</creatorcontrib><creatorcontrib>Sugita, Yoshiki</creatorcontrib><creatorcontrib>Yanagisawa, Masashi</creatorcontrib><creatorcontrib>Goto, Katsutoshi</creatorcontrib><creatorcontrib>Masaki, Tomoh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cardiovascular pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimura, Sadao</au><au>Kasuya, Yoshitoshi</au><au>Sawamura, Tatsuya</au><au>Shinmi, Osamu</au><au>Sugita, Yoshiki</au><au>Yanagisawa, Masashi</au><au>Goto, Katsutoshi</au><au>Masaki, Tomoh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conversion of Big Endothelin-1 to 21-Residue Endothelin-1 Is Essential for Expression of Full Vasoconstrictor Activity: Structure-Activity Relationships of Big Endothelin-1</atitle><jtitle>Journal of cardiovascular pharmacology</jtitle><addtitle>J Cardiovasc Pharmacol</addtitle><date>1989</date><risdate>1989</risdate><volume>13 Suppl 5</volume><spage>S5</spage><epage>7</epage><pages>S5-7</pages><issn>0160-2446</issn><eissn>1533-4023</eissn><abstract>SUMMARYThe vasoconstrictor activities of porcine big endothelin-1 (big ET-1), a 39-residue intermediate predicted from cDNA sequence analysis, and of its shorter derivative, big ET-1 [1–25], were characterized in vitro by measuring the contraction of porcine coronary artery strips. Synthetic big ET-1 [1–39] and big ET-1 [1–25] induced a slow developing, long-lasting, and strong vasoconstriction as in the case of 21-residue ET-1. However, the contractile molar potencies of big ET-1 [1–39] and big ET-1 [1–25] were approximately 140-and 50-fold lower than that of ET-1, respectively. These results indicate that the conversion of big ET-1 to “mature” ET-1 is essential for the expression of the full vasoconstrictor activity, suggesting the physiological importance of the unusual proteolytic processing catalyzed by the putative “ET converting enzyme.”</abstract><cop>United States</cop><pub>Lippincott-Raven Publishers</pub><pmid>2473327</pmid><doi>10.1097/00005344-198900135-00003</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0160-2446 |
| ispartof | Journal of cardiovascular pharmacology, 1989, Vol.13 Suppl 5, p.S5-7 |
| issn | 0160-2446 1533-4023 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79081145 |
| source | MEDLINE; Journals@Ovid LWW Legacy Archive; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Endothelins In Vitro Techniques Muscle Contraction - drug effects Muscle, Smooth, Vascular - drug effects Peptides - analysis Peptides - pharmacology Structure-Activity Relationship Swine Vasoconstriction - drug effects |
| title | Conversion of Big Endothelin-1 to 21-Residue Endothelin-1 Is Essential for Expression of Full Vasoconstrictor Activity: Structure-Activity Relationships of Big Endothelin-1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T12%3A14%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Conversion%20of%20Big%20Endothelin-1%20to%2021-Residue%20Endothelin-1%20Is%20Essential%20for%20Expression%20of%20Full%20Vasoconstrictor%20Activity:%20Structure-Activity%20Relationships%20of%20Big%20Endothelin-1&rft.jtitle=Journal%20of%20cardiovascular%20pharmacology&rft.au=Kimura,%20Sadao&rft.date=1989&rft.volume=13%20Suppl%205&rft.spage=S5&rft.epage=7&rft.pages=S5-7&rft.issn=0160-2446&rft.eissn=1533-4023&rft_id=info:doi/10.1097/00005344-198900135-00003&rft_dat=%3Cproquest_cross%3E79081145%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79081145&rft_id=info:pmid/2473327&rfr_iscdi=true |