Ribosomal transcription units integrated via T‐DNA transformation associate with the nucleolus and do not require upstream repeat sequences for activity in Arabidopsis thaliana
Ribosomal repeat units of Arabidopsis thaliana were introduced into the A. thaliana genome via Agrobacterium‐mediated transformation. Ribosomal transgenes integrated into chromosomal regions outside the nucleolus organizers. Cytological data suggest that the transgenes associate with a nucleolus. To...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 1997-05, Vol.11 (5), p.1007-1016 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Wanzenböck, Eva‐Maria Schöfer, Christian Schweizer, Dieter Bachmair, Andreas |
| description | Ribosomal repeat units of Arabidopsis thaliana were introduced into the A. thaliana genome via Agrobacterium‐mediated transformation. Ribosomal transgenes integrated into chromosomal regions outside the nucleolus organizers. Cytological data suggest that the transgenes associate with a nucleolus. To allow detection of transgenic rRNA, a short extension was inserted into the V1 variable region of the 25S ribosomal gene. The RNA transcript from the transgene undergoes a series of maturation steps, including correct processing of the 5′ end of 25S rRNA. Using primer extension analysis, expression of a complete rDNA repeat unit was compared with the activity of a repeat unit lacking a sequence called ‘upstream Sal repeats’. No qualitative or quantitative differences were detected, suggesting that upstream repeat sequences of the rDNA intergenic region do not act as transcriptional enhancers for RNA polymerase I in A. thaliana. |
| doi_str_mv | 10.1046/j.1365-313X.1997.11051007.x |
| format | Article |
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Ribosomal transgenes integrated into chromosomal regions outside the nucleolus organizers. Cytological data suggest that the transgenes associate with a nucleolus. To allow detection of transgenic rRNA, a short extension was inserted into the V1 variable region of the 25S ribosomal gene. The RNA transcript from the transgene undergoes a series of maturation steps, including correct processing of the 5′ end of 25S rRNA. Using primer extension analysis, expression of a complete rDNA repeat unit was compared with the activity of a repeat unit lacking a sequence called ‘upstream Sal repeats’. 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Psychology ; Gene Expression ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Plants, Genetically Modified ; Repetitive Sequences, Nucleic Acid ; RNA Polymerase I - metabolism ; RNA Processing, Post-Transcriptional ; RNA, Ribosomal - biosynthesis ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. 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Ribosomal transgenes integrated into chromosomal regions outside the nucleolus organizers. Cytological data suggest that the transgenes associate with a nucleolus. To allow detection of transgenic rRNA, a short extension was inserted into the V1 variable region of the 25S ribosomal gene. The RNA transcript from the transgene undergoes a series of maturation steps, including correct processing of the 5′ end of 25S rRNA. Using primer extension analysis, expression of a complete rDNA repeat unit was compared with the activity of a repeat unit lacking a sequence called ‘upstream Sal repeats’. No qualitative or quantitative differences were detected, suggesting that upstream repeat sequences of the rDNA intergenic region do not act as transcriptional enhancers for RNA polymerase I in A. thaliana.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Compartmentation</subject><subject>Cell Nucleolus - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Plants, Genetically Modified</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA Polymerase I - metabolism</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>RNA, Ribosomal - biosynthesis</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Gene Expression</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Plants, Genetically Modified</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>RNA Polymerase I - metabolism</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>RNA, Ribosomal - biosynthesis</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wanzenböck, Eva‐Maria</creatorcontrib><creatorcontrib>Schöfer, Christian</creatorcontrib><creatorcontrib>Schweizer, Dieter</creatorcontrib><creatorcontrib>Bachmair, Andreas</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wanzenböck, Eva‐Maria</au><au>Schöfer, Christian</au><au>Schweizer, Dieter</au><au>Bachmair, Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ribosomal transcription units integrated via T‐DNA transformation associate with the nucleolus and do not require upstream repeat sequences for activity in Arabidopsis thaliana</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>1997-05</date><risdate>1997</risdate><volume>11</volume><issue>5</issue><spage>1007</spage><epage>1016</epage><pages>1007-1016</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Ribosomal repeat units of Arabidopsis thaliana were introduced into the A. thaliana genome via Agrobacterium‐mediated transformation. Ribosomal transgenes integrated into chromosomal regions outside the nucleolus organizers. Cytological data suggest that the transgenes associate with a nucleolus. To allow detection of transgenic rRNA, a short extension was inserted into the V1 variable region of the 25S ribosomal gene. The RNA transcript from the transgene undergoes a series of maturation steps, including correct processing of the 5′ end of 25S rRNA. Using primer extension analysis, expression of a complete rDNA repeat unit was compared with the activity of a repeat unit lacking a sequence called ‘upstream Sal repeats’. No qualitative or quantitative differences were detected, suggesting that upstream repeat sequences of the rDNA intergenic region do not act as transcriptional enhancers for RNA polymerase I in A. thaliana.</abstract><cop>Osney Mead, Oxford OX2 0EL, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9193072</pmid><doi>10.1046/j.1365-313X.1997.11051007.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis - genetics Arabidopsis thaliana Base Sequence Biological and medical sciences Cell Compartmentation Cell Nucleolus - genetics DNA, Bacterial - genetics DNA, Ribosomal - genetics Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Gene Expression Molecular and cellular biology Molecular genetics Molecular Sequence Data Plants, Genetically Modified Repetitive Sequences, Nucleic Acid RNA Polymerase I - metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal - biosynthesis Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transformation, Genetic |
| title | Ribosomal transcription units integrated via T‐DNA transformation associate with the nucleolus and do not require upstream repeat sequences for activity in Arabidopsis thaliana |
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