Toxoplasma gondii recombinant antigens H4 and H11: Use in ELISAs for detection of toxoplasmosis in swine

Toxoplasma gondii recombinant antigens H4 and H11 were assessed for their potential for use in ELISA for diagnosis of toxoplasmosis in swine. The antigens were evaluated with sera from young pigs experimentally infected with T. gondii. Results were compared with ELISAs based on a native T. gondii an...

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Veröffentlicht in:	Veterinary parasitology 1997-06, Vol.70 (1), p.1-11
Hauptverfasser:	Andrews, C.D., Dubey, J.P., Tenter, A.M., Webert, D.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Protozoan - blood ANTIGENE ANTIGENOS ANTIGENS Antigens, Protozoan - immunology CERDO Diagnosis ELISA Enzyme-Linked Immunosorbent Assay - veterinary Immunoassay IMMUNOLOGICAL TECHNIQUES PORCIN Protozoa Recombinant proteins Recombinant Proteins - immunology SWINE Swine Diseases - diagnosis TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS TEST ELISA Toxoplasma - immunology TOXOPLASMA GONDII TOXOPLASMOSE TOXOPLASMOSIS Toxoplasmosis, Animal - diagnosis Zoonosis
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Zusammenfassung:	Toxoplasma gondii recombinant antigens H4 and H11 were assessed for their potential for use in ELISA for diagnosis of toxoplasmosis in swine. The antigens were evaluated with sera from young pigs experimentally infected with T. gondii. Results were compared with ELISAs based on a native T. gondii antigen extract. Although recombinant antigen ELISAs showed a sharp rise in response with some sera very early after infection, they were relatively non-reactive with late (chronic) infection sera.
ISSN:	0304-4017 1873-2550
DOI:	10.1016/S0304-4017(96)01154-5