A sensitive nonisotopic hybridization assay for HIV-1 DNA

We have developed a microtiter-based sandwich hybridization assay for the detection of low copy number HIV-1 sequences. The assay employs a capture DNA sequence covalently coupled to microtiter wells through linker arms. The detection probe is a biotin-labeled DNA fragment derived from sequences adj...

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Veröffentlicht in:	Analytical biochemistry 1989-02, Vol.177 (1), p.27-32
Hauptverfasser:	Keller, George H., Huang, Dao-Pei, Manak, Mark M.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Biological and medical sciences Biotechnology DNA Probes DNA, Viral - analysis DNA-Directed DNA Polymerase Fundamental and applied biological sciences. Psychology Gene Amplification Genetic engineering Genetic technics Genetics HIV-1 - genetics Methods Methods. Procedures. Technologies Microbiology Miscellaneous Nucleic Acid Hybridization Synthetic digonucleotides and genes. Sequencing Taq Polymerase Virology
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