Suppression of Neutral Cholesterol Ester Hydrolase Activity by Antisense DNA of Hormone-Sensitive Lipase
In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) from a molecular perspective, Chinese hamster ovary (CHO) cells were transfected with rat antisense hormone-sensitive lipase cDNA, and three different cell lines,...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1997-04, Vol.233 (3), p.655-657 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Osuga, Jun-ichi Ishibashi, Shun Shimano, Hitoshi Inaba, Toshimori Kawamura, Masako Yazaki, Yoshio Yamada, Nobuhiro |
| description | In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) from a molecular perspective, Chinese hamster ovary (CHO) cells were transfected with rat antisense hormone-sensitive lipase cDNA, and three different cell lines, designated as anti-HSL, were established. NCEH activity in anti-HSL cells was reduced to approximately 50% of that in control CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. These results suggest that the HSL gene has a function of NCEH as well. |
| doi_str_mv | 10.1006/bbrc.1997.6518 |
| format | Article |
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NCEH activity in anti-HSL cells was reduced to approximately 50% of that in control CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. 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NCEH activity in anti-HSL cells was reduced to approximately 50% of that in control CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. These results suggest that the HSL gene has a function of NCEH as well.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>CHO Cells</subject><subject>Cholesterol - metabolism</subject><subject>Cholesterol Esters - metabolism</subject><subject>Cricetinae</subject><subject>DNA Primers - genetics</subject><subject>DNA, Antisense - genetics</subject><subject>DNA, Antisense - pharmacology</subject><subject>DNA, Complementary - genetics</subject><subject>Esterification</subject><subject>Genetic Vectors</subject><subject>Rats</subject><subject>Sterol Esterase - antagonists & inhibitors</subject><subject>Sterol Esterase - genetics</subject><subject>Sterol Esterase - metabolism</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EgvKxsiF5Yks5O1_2WJVCkSoYAInNcpyLMErjYCdI_fc4asXGdPbdc690DyHXDOYMoLirKm_mTMpyXuRMHJEZAwkJZ5AdkxlEIuGSfZyR8xC-ABjLCnlKTiUrhAQxI5-vY997DMG6jrqGPuM4eN3S5adrMQzoXUtXU6XrXR0_OiBdmMH-2GFHqx1ddIMN2MXu_fNiClg7v3UdJq-xaSOHdGP7uHVJThrdBrw61Avy_rB6W66Tzcvj03KxSUyayiGpc6ZLXuSSQwZVfJei5rUWnKcpY3mT6dyIuuFgyoqXAI3mvBG5Zihl3mCWXpDbfW7v3fcYT1BbGwy2re7QjUGVEjIBICM434PGuxA8Nqr3dqv9TjFQk1o1qVWTWjWpjQs3h-Sx2mL9hx9cxrnYzzGe92PRq2AsdgZr69EMqnb2v-hf6RmIiw</recordid><startdate>19970428</startdate><enddate>19970428</enddate><creator>Osuga, Jun-ichi</creator><creator>Ishibashi, Shun</creator><creator>Shimano, Hitoshi</creator><creator>Inaba, Toshimori</creator><creator>Kawamura, Masako</creator><creator>Yazaki, Yoshio</creator><creator>Yamada, Nobuhiro</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970428</creationdate><title>Suppression of Neutral Cholesterol Ester Hydrolase Activity by Antisense DNA of Hormone-Sensitive Lipase</title><author>Osuga, Jun-ichi ; Ishibashi, Shun ; Shimano, Hitoshi ; Inaba, Toshimori ; Kawamura, Masako ; Yazaki, Yoshio ; Yamada, Nobuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-d51a726592040b1a778d2da82233115f4a5c8df20c7b2700fa22f85a1e995fe43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>CHO Cells</topic><topic>Cholesterol - metabolism</topic><topic>Cholesterol Esters - metabolism</topic><topic>Cricetinae</topic><topic>DNA Primers - genetics</topic><topic>DNA, Antisense - genetics</topic><topic>DNA, Antisense - pharmacology</topic><topic>DNA, Complementary - genetics</topic><topic>Esterification</topic><topic>Genetic Vectors</topic><topic>Rats</topic><topic>Sterol Esterase - antagonists & inhibitors</topic><topic>Sterol Esterase - genetics</topic><topic>Sterol Esterase - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osuga, Jun-ichi</creatorcontrib><creatorcontrib>Ishibashi, Shun</creatorcontrib><creatorcontrib>Shimano, Hitoshi</creatorcontrib><creatorcontrib>Inaba, Toshimori</creatorcontrib><creatorcontrib>Kawamura, Masako</creatorcontrib><creatorcontrib>Yazaki, Yoshio</creatorcontrib><creatorcontrib>Yamada, Nobuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osuga, Jun-ichi</au><au>Ishibashi, Shun</au><au>Shimano, Hitoshi</au><au>Inaba, Toshimori</au><au>Kawamura, Masako</au><au>Yazaki, Yoshio</au><au>Yamada, Nobuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of Neutral Cholesterol Ester Hydrolase Activity by Antisense DNA of Hormone-Sensitive Lipase</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1997-04-28</date><risdate>1997</risdate><volume>233</volume><issue>3</issue><spage>655</spage><epage>657</epage><pages>655-657</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) from a molecular perspective, Chinese hamster ovary (CHO) cells were transfected with rat antisense hormone-sensitive lipase cDNA, and three different cell lines, designated as anti-HSL, were established. 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| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1997-04, Vol.233 (3), p.655-657 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Base Sequence CHO Cells Cholesterol - metabolism Cholesterol Esters - metabolism Cricetinae DNA Primers - genetics DNA, Antisense - genetics DNA, Antisense - pharmacology DNA, Complementary - genetics Esterification Genetic Vectors Rats Sterol Esterase - antagonists & inhibitors Sterol Esterase - genetics Sterol Esterase - metabolism Transfection |
| title | Suppression of Neutral Cholesterol Ester Hydrolase Activity by Antisense DNA of Hormone-Sensitive Lipase |
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