Basic fibroblast growth factor is released from endothelial extracellular matrix in a biologically active form
Basic fibroblast growth factor (bFGF) binds to heparin‐like molecules present in the extracellular matrix (ECM) of transformed fetal bovine aortic endothelial GM 7373 cells. Binding of bFGF to ECM can be competed by heparin or heparan sulfate, and ECM‐bound bFGF can be released by treating the cells...
Gespeichert in:
| Veröffentlicht in: | Journal of cellular physiology 1989-07, Vol.140 (1), p.68-74 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 74 |
|---|---|
| container_issue | 1 |
| container_start_page | 68 |
| container_title | Journal of cellular physiology |
| container_volume | 140 |
| creator | Presta, M. Maier, J. A. M. Rusnati, M. Ragnotti, G. |
| description | Basic fibroblast growth factor (bFGF) binds to heparin‐like molecules present in the extracellular matrix (ECM) of transformed fetal bovine aortic endothelial GM 7373 cells. Binding of bFGF to ECM can be competed by heparin or heparan sulfate, and ECM‐bound bFGF can be released by treating the cells with heparinase or heparatinase. After binding to ECM, bFGF is slowly released into the medium in a biologically active form, as shown by its capacity to induce an increase of cell‐associated plasminogen activator activity and cell proliferation. The increase is prevented upon removal of ECM‐bound bFGF by a neutral 2 M NaCI wash. Soluble heparin and heparan sulfate reduce the amount of ECM‐bound bFGF released into the medium, possibly competing with ECM polysaccharides for heparinase‐like enzymes produced by endothelial cells, suggesting that these enzymes are involved in the mobilization of ECM‐bound bFGF. |
| doi_str_mv | 10.1002/jcp.1041400109 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79047298</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79047298</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5059-22b54c839dd74ad55894d83792767baf509ab6a020674c32425def596bfdb1103</originalsourceid><addsrcrecordid>eNqFkc1vEzEQxS0EKqFw5YbkC9y29ec6PkKghSpqcwBxtGa9duviXaf2hib_Pa4SteLUi8fS_N6b0TyE3lNyQglhp7d2XT-CCkIo0S_QrL6qEa1kL9GsArTRUtDX6E0pt4QQrTk_QkdMEiIkm6HxC5RgsQ9dTl2EMuHrnO6nG-zBTinjUHB20UFxPfY5DdiNfZpuXAwQsdtOGayLcRMh4wGmHLY4jBhwF1JM18FCjDtcncJfh33Kw1v0ykMs7t2hHqNfZ99-Lr43y6vzH4vPy8ZKInXDWCeFnXPd90pAL-Vci37OlWaqVR14STR0LRBGWiUsZ4LJ3nmp2873HaWEH6NPe991TncbVyYzhPKwKYwubYpRmgjF9PxZkEquhRK0gid70OZUSnberHMYIO8MJeYhCVOTME9JVMGHg_OmG1z_iB9OX_sfD30o9U4-w2hDeXLVkjIq28rpPXcfots9M9VcLFb_7dDstaFMbvuohfzHtIoraX5fnpuvq9UZWzJmFvwfMxyxEA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15394741</pqid></control><display><type>article</type><title>Basic fibroblast growth factor is released from endothelial extracellular matrix in a biologically active form</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Presta, M. ; Maier, J. A. M. ; Rusnati, M. ; Ragnotti, G.</creator><creatorcontrib>Presta, M. ; Maier, J. A. M. ; Rusnati, M. ; Ragnotti, G.</creatorcontrib><description>Basic fibroblast growth factor (bFGF) binds to heparin‐like molecules present in the extracellular matrix (ECM) of transformed fetal bovine aortic endothelial GM 7373 cells. Binding of bFGF to ECM can be competed by heparin or heparan sulfate, and ECM‐bound bFGF can be released by treating the cells with heparinase or heparatinase. After binding to ECM, bFGF is slowly released into the medium in a biologically active form, as shown by its capacity to induce an increase of cell‐associated plasminogen activator activity and cell proliferation. The increase is prevented upon removal of ECM‐bound bFGF by a neutral 2 M NaCI wash. Soluble heparin and heparan sulfate reduce the amount of ECM‐bound bFGF released into the medium, possibly competing with ECM polysaccharides for heparinase‐like enzymes produced by endothelial cells, suggesting that these enzymes are involved in the mobilization of ECM‐bound bFGF.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.1041400109</identifier><identifier>PMID: 2500452</identifier><identifier>CODEN: JCLLAX</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Aorta - cytology ; Biological and medical sciences ; Blotting, Western ; Cattle ; Cell Division - drug effects ; Cell physiology ; Cells, Cultured ; Chondroitin Sulfates - pharmacology ; Endothelium ; Extracellular Matrix - metabolism ; Fibroblast Growth Factors - metabolism ; Fundamental and applied biological sciences. Psychology ; Heparin - pharmacology ; Heparitin Sulfate - pharmacology ; Molecular and cellular biology ; Plasminogen Activators - analysis ; Recombinant Proteins - metabolism ; Responses to growth factors, tumor promotors, other factors</subject><ispartof>Journal of cellular physiology, 1989-07, Vol.140 (1), p.68-74</ispartof><rights>Copyright © 1989 Wiley‐Liss, Inc.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5059-22b54c839dd74ad55894d83792767baf509ab6a020674c32425def596bfdb1103</citedby><cites>FETCH-LOGICAL-c5059-22b54c839dd74ad55894d83792767baf509ab6a020674c32425def596bfdb1103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.1041400109$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.1041400109$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19512156$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2500452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Presta, M.</creatorcontrib><creatorcontrib>Maier, J. A. M.</creatorcontrib><creatorcontrib>Rusnati, M.</creatorcontrib><creatorcontrib>Ragnotti, G.</creatorcontrib><title>Basic fibroblast growth factor is released from endothelial extracellular matrix in a biologically active form</title><title>Journal of cellular physiology</title><addtitle>J. Cell. Physiol</addtitle><description>Basic fibroblast growth factor (bFGF) binds to heparin‐like molecules present in the extracellular matrix (ECM) of transformed fetal bovine aortic endothelial GM 7373 cells. Binding of bFGF to ECM can be competed by heparin or heparan sulfate, and ECM‐bound bFGF can be released by treating the cells with heparinase or heparatinase. After binding to ECM, bFGF is slowly released into the medium in a biologically active form, as shown by its capacity to induce an increase of cell‐associated plasminogen activator activity and cell proliferation. The increase is prevented upon removal of ECM‐bound bFGF by a neutral 2 M NaCI wash. Soluble heparin and heparan sulfate reduce the amount of ECM‐bound bFGF released into the medium, possibly competing with ECM polysaccharides for heparinase‐like enzymes produced by endothelial cells, suggesting that these enzymes are involved in the mobilization of ECM‐bound bFGF.</description><subject>Animals</subject><subject>Aorta - cytology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cattle</subject><subject>Cell Division - drug effects</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Chondroitin Sulfates - pharmacology</subject><subject>Endothelium</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibroblast Growth Factors - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heparin - pharmacology</subject><subject>Heparitin Sulfate - pharmacology</subject><subject>Molecular and cellular biology</subject><subject>Plasminogen Activators - analysis</subject><subject>Recombinant Proteins - metabolism</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EKqFw5YbkC9y29ec6PkKghSpqcwBxtGa9duviXaf2hib_Pa4SteLUi8fS_N6b0TyE3lNyQglhp7d2XT-CCkIo0S_QrL6qEa1kL9GsArTRUtDX6E0pt4QQrTk_QkdMEiIkm6HxC5RgsQ9dTl2EMuHrnO6nG-zBTinjUHB20UFxPfY5DdiNfZpuXAwQsdtOGayLcRMh4wGmHLY4jBhwF1JM18FCjDtcncJfh33Kw1v0ykMs7t2hHqNfZ99-Lr43y6vzH4vPy8ZKInXDWCeFnXPd90pAL-Vci37OlWaqVR14STR0LRBGWiUsZ4LJ3nmp2873HaWEH6NPe991TncbVyYzhPKwKYwubYpRmgjF9PxZkEquhRK0gid70OZUSnberHMYIO8MJeYhCVOTME9JVMGHg_OmG1z_iB9OX_sfD30o9U4-w2hDeXLVkjIq28rpPXcfots9M9VcLFb_7dDstaFMbvuohfzHtIoraX5fnpuvq9UZWzJmFvwfMxyxEA</recordid><startdate>198907</startdate><enddate>198907</enddate><creator>Presta, M.</creator><creator>Maier, J. A. M.</creator><creator>Rusnati, M.</creator><creator>Ragnotti, G.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198907</creationdate><title>Basic fibroblast growth factor is released from endothelial extracellular matrix in a biologically active form</title><author>Presta, M. ; Maier, J. A. M. ; Rusnati, M. ; Ragnotti, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5059-22b54c839dd74ad55894d83792767baf509ab6a020674c32425def596bfdb1103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Aorta - cytology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cattle</topic><topic>Cell Division - drug effects</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Chondroitin Sulfates - pharmacology</topic><topic>Endothelium</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibroblast Growth Factors - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heparin - pharmacology</topic><topic>Heparitin Sulfate - pharmacology</topic><topic>Molecular and cellular biology</topic><topic>Plasminogen Activators - analysis</topic><topic>Recombinant Proteins - metabolism</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Presta, M.</creatorcontrib><creatorcontrib>Maier, J. A. M.</creatorcontrib><creatorcontrib>Rusnati, M.</creatorcontrib><creatorcontrib>Ragnotti, G.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Presta, M.</au><au>Maier, J. A. M.</au><au>Rusnati, M.</au><au>Ragnotti, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Basic fibroblast growth factor is released from endothelial extracellular matrix in a biologically active form</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J. Cell. Physiol</addtitle><date>1989-07</date><risdate>1989</risdate><volume>140</volume><issue>1</issue><spage>68</spage><epage>74</epage><pages>68-74</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><coden>JCLLAX</coden><abstract>Basic fibroblast growth factor (bFGF) binds to heparin‐like molecules present in the extracellular matrix (ECM) of transformed fetal bovine aortic endothelial GM 7373 cells. Binding of bFGF to ECM can be competed by heparin or heparan sulfate, and ECM‐bound bFGF can be released by treating the cells with heparinase or heparatinase. After binding to ECM, bFGF is slowly released into the medium in a biologically active form, as shown by its capacity to induce an increase of cell‐associated plasminogen activator activity and cell proliferation. The increase is prevented upon removal of ECM‐bound bFGF by a neutral 2 M NaCI wash. Soluble heparin and heparan sulfate reduce the amount of ECM‐bound bFGF released into the medium, possibly competing with ECM polysaccharides for heparinase‐like enzymes produced by endothelial cells, suggesting that these enzymes are involved in the mobilization of ECM‐bound bFGF.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2500452</pmid><doi>10.1002/jcp.1041400109</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9541 |
| ispartof | Journal of cellular physiology, 1989-07, Vol.140 (1), p.68-74 |
| issn | 0021-9541 1097-4652 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79047298 |
| source | MEDLINE; Wiley Journals |
| subjects | Animals Aorta - cytology Biological and medical sciences Blotting, Western Cattle Cell Division - drug effects Cell physiology Cells, Cultured Chondroitin Sulfates - pharmacology Endothelium Extracellular Matrix - metabolism Fibroblast Growth Factors - metabolism Fundamental and applied biological sciences. Psychology Heparin - pharmacology Heparitin Sulfate - pharmacology Molecular and cellular biology Plasminogen Activators - analysis Recombinant Proteins - metabolism Responses to growth factors, tumor promotors, other factors |
| title | Basic fibroblast growth factor is released from endothelial extracellular matrix in a biologically active form |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T02%3A35%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Basic%20fibroblast%20growth%20factor%20is%20released%20from%20endothelial%20extracellular%20matrix%20in%20a%20biologically%20active%20form&rft.jtitle=Journal%20of%20cellular%20physiology&rft.au=Presta,%20M.&rft.date=1989-07&rft.volume=140&rft.issue=1&rft.spage=68&rft.epage=74&rft.pages=68-74&rft.issn=0021-9541&rft.eissn=1097-4652&rft.coden=JCLLAX&rft_id=info:doi/10.1002/jcp.1041400109&rft_dat=%3Cproquest_cross%3E79047298%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15394741&rft_id=info:pmid/2500452&rfr_iscdi=true |