Differences in Protein Structure of Xanthine Dehydrogenase and Xanthine Oxidase Revealed by Reconstitution with Flavin Active Site Probes

The native flavin, FAD, was removed from chicken liver xanthine dehydrogenase and milk xanthine oxidase by incubation with CaCl2. The deflavoenzymes, still retaining their molybdopterin and iron-sulfur prosthetic groups, were reconstituted with a series of FAD derivatives containing chemically react...

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Veröffentlicht in:	The Journal of biological chemistry 1989-06, Vol.264 (18), p.10567-10573
Hauptverfasser:	Massey, V, Schopfer, L M, Nishino, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Binding Sites Biological and medical sciences Cattle Chickens Enzymes and enzyme inhibitors Female flavin Flavin-Adenine Dinucleotide - analogs & derivatives Flavin-Adenine Dinucleotide - metabolism Fundamental and applied biological sciences. Psychology Ketone Oxidoreductases - metabolism Kinetics Liver - enzymology milk Milk - enzymology Oxidoreductases Protein Binding Spectrophotometry Xanthine Dehydrogenase - metabolism xanthine oxidase Xanthine Oxidase - metabolism
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