Purification of F(ab′) 2 anti-snake venom by caprylic acid: A fast method for obtaining IgG fragments with high neutralization activity, purity and yield

Pooled horse plasma containing antibodies against Crotalus durissus terrificus whole venom were digested with pepsin at an enzyme-substrate ratio of 8:1, pH 3.1, for 40 min and the F(ab′) 2M fragments purified by adding 8.7% caprylic acid (pH 5.0). For comparison, F(ab′) 2B purified by precipitation...

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Veröffentlicht in:	Toxicon (Oxford) 1989, Vol.27 (3), p.297-303
Hauptverfasser:	dos Santos, M.C., D'Império Lima, M.R., Furtado, G.C., Colletto, G.M.D.D., Kipnis, T.L., Dias da Silva, W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal poisons toxicology. Antivenoms Animals Antibody Specificity Antivenins - isolation & purification Biological and medical sciences Blotting, Western Caprylates - pharmacology Crotalid Venoms - immunology Electrophoresis, Polyacrylamide Gel Horses - immunology Immunoglobulin Fab Fragments - isolation & purification Immunoglobulin G - isolation & purification Medical sciences Mice Mice, Inbred BALB C Mice, Inbred C57BL Neutralization Tests Toxicology
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