Potential of electrospray mass spectrometry for quantifying glycohemoglobin
An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent...
Gespeichert in:
| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 1997-05, Vol.43 (5), p.771-778 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 778 |
|---|---|
| container_issue | 5 |
| container_start_page | 771 |
| container_title | Clinical chemistry (Baltimore, Md.) |
| container_volume | 43 |
| creator | Roberts, Norman B Green, Brian N Morris, Michael |
| description | An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was approximately 3 min. The imprecision (CV) of the method was < 5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6-14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the alpha- and beta-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the alpha- and beta-chains was observed. |
| doi_str_mv | 10.1093/clinchem/43.5.771 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79031370</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79031370</sourcerecordid><originalsourceid>FETCH-LOGICAL-c464t-a66dad07ed131ca240b06f2fd6559016387dfb37f7731412b28df23eb942b9783</originalsourceid><addsrcrecordid>eNo9kE9P4zAQxS0EglL2A3BAygG4pfXYjp0cEWL_CKTdA5wtx7FbIycudqoq3x4vLT2NZuY372keQteAF4AbutTeDXpt-iWji2ohBJygGVQUl3XF4RTNMMZN2QATF-gypffcMlHzc3TeAOeE4hl6_hdGM4xO-SLYwnijxxjSJqqp6FVKRdp8TXozxqmwIRYfW5VxO7lhVaz8pEO2DysfWjdcoTOrfDI_DnWO3n4-vT7-Ll_-_vrz-PBSasbZWCrOO9VhYTqgoBVhuMXcEtvxqmowcFqLzrZUWCEoMCAtqTtLqGkbRtpG1HSO7ve6mxg-tiaNsndJG-_VYMI2SdFgClTgDMIe1PmnFI2Vm-h6FScJWP4PUH4HKBmVlcwB5pubg_i27U13vDgklve3h71KWnkb1aBdOmKEVzVQkrG7PbZ2q_XORSNTr7zPoiB3u93R7hMsHIj-</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79031370</pqid></control><display><type>article</type><title>Potential of electrospray mass spectrometry for quantifying glycohemoglobin</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><creator>Roberts, Norman B ; Green, Brian N ; Morris, Michael</creator><creatorcontrib>Roberts, Norman B ; Green, Brian N ; Morris, Michael</creatorcontrib><description>An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was approximately 3 min. The imprecision (CV) of the method was < 5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6-14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the alpha- and beta-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the alpha- and beta-chains was observed.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1093/clinchem/43.5.771</identifier><identifier>PMID: 9166230</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Biological and medical sciences ; Chromatography, Affinity ; Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; Diabetes Mellitus - blood ; Diabetes. Impaired glucose tolerance ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Globins - metabolism ; Glycated Hemoglobin A - analysis ; Glycosylation ; Humans ; Mass Spectrometry - methods ; Medical sciences ; Sensitivity and Specificity</subject><ispartof>Clinical chemistry (Baltimore, Md.), 1997-05, Vol.43 (5), p.771-778</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-a66dad07ed131ca240b06f2fd6559016387dfb37f7731412b28df23eb942b9783</citedby><cites>FETCH-LOGICAL-c464t-a66dad07ed131ca240b06f2fd6559016387dfb37f7731412b28df23eb942b9783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23909,23910,25118,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2658132$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9166230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roberts, Norman B</creatorcontrib><creatorcontrib>Green, Brian N</creatorcontrib><creatorcontrib>Morris, Michael</creatorcontrib><title>Potential of electrospray mass spectrometry for quantifying glycohemoglobin</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was approximately 3 min. The imprecision (CV) of the method was < 5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6-14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the alpha- and beta-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the alpha- and beta-chains was observed.</description><subject>Biological and medical sciences</subject><subject>Chromatography, Affinity</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Ion Exchange</subject><subject>Diabetes Mellitus - blood</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Globins - metabolism</subject><subject>Glycated Hemoglobin A - analysis</subject><subject>Glycosylation</subject><subject>Humans</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Sensitivity and Specificity</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9P4zAQxS0EglL2A3BAygG4pfXYjp0cEWL_CKTdA5wtx7FbIycudqoq3x4vLT2NZuY372keQteAF4AbutTeDXpt-iWji2ohBJygGVQUl3XF4RTNMMZN2QATF-gypffcMlHzc3TeAOeE4hl6_hdGM4xO-SLYwnijxxjSJqqp6FVKRdp8TXozxqmwIRYfW5VxO7lhVaz8pEO2DysfWjdcoTOrfDI_DnWO3n4-vT7-Ll_-_vrz-PBSasbZWCrOO9VhYTqgoBVhuMXcEtvxqmowcFqLzrZUWCEoMCAtqTtLqGkbRtpG1HSO7ve6mxg-tiaNsndJG-_VYMI2SdFgClTgDMIe1PmnFI2Vm-h6FScJWP4PUH4HKBmVlcwB5pubg_i27U13vDgklve3h71KWnkb1aBdOmKEVzVQkrG7PbZ2q_XORSNTr7zPoiB3u93R7hMsHIj-</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>Roberts, Norman B</creator><creator>Green, Brian N</creator><creator>Morris, Michael</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970501</creationdate><title>Potential of electrospray mass spectrometry for quantifying glycohemoglobin</title><author>Roberts, Norman B ; Green, Brian N ; Morris, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-a66dad07ed131ca240b06f2fd6559016387dfb37f7731412b28df23eb942b9783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Chromatography, Affinity</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Ion Exchange</topic><topic>Diabetes Mellitus - blood</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Globins - metabolism</topic><topic>Glycated Hemoglobin A - analysis</topic><topic>Glycosylation</topic><topic>Humans</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roberts, Norman B</creatorcontrib><creatorcontrib>Green, Brian N</creatorcontrib><creatorcontrib>Morris, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roberts, Norman B</au><au>Green, Brian N</au><au>Morris, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential of electrospray mass spectrometry for quantifying glycohemoglobin</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>43</volume><issue>5</issue><spage>771</spage><epage>778</epage><pages>771-778</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was approximately 3 min. The imprecision (CV) of the method was < 5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6-14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the alpha- and beta-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the alpha- and beta-chains was observed.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>9166230</pmid><doi>10.1093/clinchem/43.5.771</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9147 |
| ispartof | Clinical chemistry (Baltimore, Md.), 1997-05, Vol.43 (5), p.771-778 |
| issn | 0009-9147 1530-8561 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79031370 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Biological and medical sciences Chromatography, Affinity Chromatography, High Pressure Liquid Chromatography, Ion Exchange Diabetes Mellitus - blood Diabetes. Impaired glucose tolerance Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Globins - metabolism Glycated Hemoglobin A - analysis Glycosylation Humans Mass Spectrometry - methods Medical sciences Sensitivity and Specificity |
| title | Potential of electrospray mass spectrometry for quantifying glycohemoglobin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T17%3A22%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Potential%20of%20electrospray%20mass%20spectrometry%20for%20quantifying%20glycohemoglobin&rft.jtitle=Clinical%20chemistry%20(Baltimore,%20Md.)&rft.au=Roberts,%20Norman%20B&rft.date=1997-05-01&rft.volume=43&rft.issue=5&rft.spage=771&rft.epage=778&rft.pages=771-778&rft.issn=0009-9147&rft.eissn=1530-8561&rft.coden=CLCHAU&rft_id=info:doi/10.1093/clinchem/43.5.771&rft_dat=%3Cproquest_cross%3E79031370%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79031370&rft_id=info:pmid/9166230&rfr_iscdi=true |