Transcriptional regulation of Notch and Delta: requirement for neuroblast segregation in Drosophila

Segregation of a single neural precursor from each proneural cluster in Drosophila relies on Notch-mediated lateral signalling. Studies concerning the spacing of precursors for the microchaetes of the peripheral nervous system suggested the existence of a regulatory loop between Notch and its ligand...

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Veröffentlicht in:Development (Cambridge) 1997-05, Vol.124 (10), p.2015-2025
Hauptverfasser: Seugnet, L, Simpson, P, Haenlin, M
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creator Seugnet, L
Simpson, P
Haenlin, M
description Segregation of a single neural precursor from each proneural cluster in Drosophila relies on Notch-mediated lateral signalling. Studies concerning the spacing of precursors for the microchaetes of the peripheral nervous system suggested the existence of a regulatory loop between Notch and its ligand Delta within each cell that is under transcriptional control. Activation of Notch leads to repression of the achaete-scute genes which themselves regulate transcription of Delta, perhaps directly. Here we have tested a requirement for transcriptional regulation of Notch and/or Delta during neuroblast segregation in embryos, by providing Notch and Delta ubiquitously at uniform levels. Neuroblast segregation occurs normally under conditions of uniform Notch expression. Under conditions of uniform Delta expression, a single neuroblast segregates from each proneural group in 80% of the cases, more than one in the remaining 20%. Thus transcriptional regulation of Delta is largely dispensable. We discuss the possibility that segregation of single precursors in the central nervous system may rely on a heterogeneous distribution of neural potential between different cells of the proneural group. Notch signalling would enable all cells to mutually repress each other and only a cell with an elevated neural potential could overcome this repression.
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists
subjects Animals
Basic Helix-Loop-Helix Transcription Factors
DNA-Binding Proteins - analysis
DNA-Binding Proteins - metabolism
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins
Ectoderm - chemistry
Ectoderm - cytology
Gene Expression Regulation, Developmental - genetics
Homeodomain Proteins - analysis
Intracellular Signaling Peptides and Proteins
Membrane Proteins - analysis
Membrane Proteins - genetics
Nervous System - chemistry
Nervous System - cytology
Nervous System - embryology
Neurons - chemistry
Neurons - cytology
Promoter Regions, Genetic - genetics
Receptors, Notch
Receptors, Steroid - analysis
Recombinant Fusion Proteins
Transcription Factors - analysis
Transcription Factors - metabolism
Transcription, Genetic - genetics
Transgenes
title Transcriptional regulation of Notch and Delta: requirement for neuroblast segregation in Drosophila
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