Identification of a lineage of multipotent hematopoietic progenitors
All multipotent hematopoietic progenitors in C57BL-Thy-1.1 bone marrow are divided among three subpopulations of Thy-1.1(lo) Sca-1+ Lin(-/lo) c-kit+ cells: long-term reconstituting Mac-1- CD4- c-kit+ cells and transiently reconstituting Mac-1(lo) CD4- or Mac-1(lo) CD4(lo) cells. This study shows tha...
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| Veröffentlicht in: | Development (Cambridge) 1997-05, Vol.124 (10), p.1929-1939 |
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| container_end_page | 1939 |
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| container_issue | 10 |
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| container_title | Development (Cambridge) |
| container_volume | 124 |
| creator | Morrison, S J Wandycz, A M Hemmati, H D Wright, D E Weissman, I L |
| description | All multipotent hematopoietic progenitors in C57BL-Thy-1.1 bone marrow are divided among three subpopulations of Thy-1.1(lo) Sca-1+ Lin(-/lo) c-kit+ cells: long-term reconstituting Mac-1- CD4- c-kit+ cells and transiently reconstituting Mac-1(lo) CD4- or Mac-1(lo) CD4(lo) cells. This study shows that the same populations, with similar functional activities, exist in mice whose hematopoietic systems were reconstituted by hematopoietic stem cells after lethal irradiation. We demonstrate that these populations form a lineage of multipotent progenitors from long-term self-renewing stem cells to the most mature multipotent progenitor population. In reconstituted mice, Mac-1- CD4- c-kit+ cells gave rise to Mac-1(lo) CD4- cells, which gave rise to Mac-1(lo) CD4(lo) cells. Mac-1- CD4- c-kit+ cells had long-term self-renewal potential, with each cell being capable of giving rise to more than 10(4) functionally similar Mac-1- CD4- c-kit+ cells. At least half of Mac-1(lo) CD4- cells had transient self-renewal potential, detected in the spleen 7 days after reconstitution. Mac-1(lo) CD4(lo) cells did not have detectable self-renewal potential. The identification of a lineage of multipotent progenitors provides an important tool for identifying genes that regulate self-renewal and lineage commitment. |
| doi_str_mv | 10.1242/dev.124.10.1929 |
| format | Article |
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This study shows that the same populations, with similar functional activities, exist in mice whose hematopoietic systems were reconstituted by hematopoietic stem cells after lethal irradiation. We demonstrate that these populations form a lineage of multipotent progenitors from long-term self-renewing stem cells to the most mature multipotent progenitor population. In reconstituted mice, Mac-1- CD4- c-kit+ cells gave rise to Mac-1(lo) CD4- cells, which gave rise to Mac-1(lo) CD4(lo) cells. Mac-1- CD4- c-kit+ cells had long-term self-renewal potential, with each cell being capable of giving rise to more than 10(4) functionally similar Mac-1- CD4- c-kit+ cells. At least half of Mac-1(lo) CD4- cells had transient self-renewal potential, detected in the spleen 7 days after reconstitution. Mac-1(lo) CD4(lo) cells did not have detectable self-renewal potential. The identification of a lineage of multipotent progenitors provides an important tool for identifying genes that regulate self-renewal and lineage commitment.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.124.10.1929</identifier><identifier>PMID: 9169840</identifier><language>eng</language><publisher>England: The Company of Biologists Limited</publisher><subject>Animals ; Bone Marrow Cells ; Bone Marrow Transplantation ; CD4 Antigens - analysis ; Cells, Cultured ; Hematopoiesis - physiology ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells - chemistry ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - radiation effects ; Macrophage-1 Antigen - analysis ; Mice ; Mice, Inbred C57BL ; Proto-Oncogene Proteins c-kit - analysis ; Spleen - cytology ; Thy-1 Antigens - analysis</subject><ispartof>Development (Cambridge), 1997-05, Vol.124 (10), p.1929-1939</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-6a536e15da9cf045af09d51d231e980507c73decca6b6c8064a5210f8ec686203</citedby><cites>FETCH-LOGICAL-c469t-6a536e15da9cf045af09d51d231e980507c73decca6b6c8064a5210f8ec686203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3678,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9169840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morrison, S J</creatorcontrib><creatorcontrib>Wandycz, A M</creatorcontrib><creatorcontrib>Hemmati, H D</creatorcontrib><creatorcontrib>Wright, D E</creatorcontrib><creatorcontrib>Weissman, I L</creatorcontrib><title>Identification of a lineage of multipotent hematopoietic progenitors</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>All multipotent hematopoietic progenitors in C57BL-Thy-1.1 bone marrow are divided among three subpopulations of Thy-1.1(lo) Sca-1+ Lin(-/lo) c-kit+ cells: long-term reconstituting Mac-1- CD4- c-kit+ cells and transiently reconstituting Mac-1(lo) CD4- or Mac-1(lo) CD4(lo) cells. This study shows that the same populations, with similar functional activities, exist in mice whose hematopoietic systems were reconstituted by hematopoietic stem cells after lethal irradiation. We demonstrate that these populations form a lineage of multipotent progenitors from long-term self-renewing stem cells to the most mature multipotent progenitor population. In reconstituted mice, Mac-1- CD4- c-kit+ cells gave rise to Mac-1(lo) CD4- cells, which gave rise to Mac-1(lo) CD4(lo) cells. Mac-1- CD4- c-kit+ cells had long-term self-renewal potential, with each cell being capable of giving rise to more than 10(4) functionally similar Mac-1- CD4- c-kit+ cells. At least half of Mac-1(lo) CD4- cells had transient self-renewal potential, detected in the spleen 7 days after reconstitution. Mac-1(lo) CD4(lo) cells did not have detectable self-renewal potential. The identification of a lineage of multipotent progenitors provides an important tool for identifying genes that regulate self-renewal and lineage commitment.</description><subject>Animals</subject><subject>Bone Marrow Cells</subject><subject>Bone Marrow Transplantation</subject><subject>CD4 Antigens - analysis</subject><subject>Cells, Cultured</subject><subject>Hematopoiesis - physiology</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Hematopoietic Stem Cells - chemistry</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - radiation effects</subject><subject>Macrophage-1 Antigen - analysis</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Proto-Oncogene Proteins c-kit - analysis</subject><subject>Spleen - cytology</subject><subject>Thy-1 Antigens - analysis</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAQxS0EKqUwMyFlYkt7dmInHlH5lCqxwBy5zjk1SuIQOyD-exJaITEx3T29372THiGXFJaUpWxV4se0LCctmTwic5pmWSwpk8dkDpJDTKWkp-TM-zcASESWzchMUiHzFObk9qnENlhjtQrWtZEzkYpq26KqcBLNUAfbuTBC0Q4bFVznLAaro653FbY2uN6fkxOjao8Xh7kgr_d3L-vHePP88LS-2cQ6FTLEQvFEIOWlktpAypUBWXJasoSizIFDprOkRK2V2Aqdg0gVZxRMjlrkgkGyINf73PH3-4A-FI31GutategGX2QSGAfg_4JUAM9zKUZwtQd177zv0RRdbxvVfxUUiqngYix4Wn70WPB4cXWIHrYNlr_8odHRX-79na12n7bHYmtd7Srrg5_CsHbdn8BvWMGHGA</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>Morrison, S J</creator><creator>Wandycz, A M</creator><creator>Hemmati, H D</creator><creator>Wright, D E</creator><creator>Weissman, I L</creator><general>The Company of Biologists Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19970501</creationdate><title>Identification of a lineage of multipotent hematopoietic progenitors</title><author>Morrison, S J ; Wandycz, A M ; Hemmati, H D ; Wright, D E ; Weissman, I L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-6a536e15da9cf045af09d51d231e980507c73decca6b6c8064a5210f8ec686203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Bone Marrow Cells</topic><topic>Bone Marrow Transplantation</topic><topic>CD4 Antigens - analysis</topic><topic>Cells, Cultured</topic><topic>Hematopoiesis - physiology</topic><topic>Hematopoietic Stem Cell Transplantation</topic><topic>Hematopoietic Stem Cells - chemistry</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - radiation effects</topic><topic>Macrophage-1 Antigen - analysis</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Proto-Oncogene Proteins c-kit - analysis</topic><topic>Spleen - cytology</topic><topic>Thy-1 Antigens - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morrison, S J</creatorcontrib><creatorcontrib>Wandycz, A M</creatorcontrib><creatorcontrib>Hemmati, H D</creatorcontrib><creatorcontrib>Wright, D E</creatorcontrib><creatorcontrib>Weissman, I L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morrison, S J</au><au>Wandycz, A M</au><au>Hemmati, H D</au><au>Wright, D E</au><au>Weissman, I L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a lineage of multipotent hematopoietic progenitors</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>124</volume><issue>10</issue><spage>1929</spage><epage>1939</epage><pages>1929-1939</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>All multipotent hematopoietic progenitors in C57BL-Thy-1.1 bone marrow are divided among three subpopulations of Thy-1.1(lo) Sca-1+ Lin(-/lo) c-kit+ cells: long-term reconstituting Mac-1- CD4- c-kit+ cells and transiently reconstituting Mac-1(lo) CD4- or Mac-1(lo) CD4(lo) cells. This study shows that the same populations, with similar functional activities, exist in mice whose hematopoietic systems were reconstituted by hematopoietic stem cells after lethal irradiation. We demonstrate that these populations form a lineage of multipotent progenitors from long-term self-renewing stem cells to the most mature multipotent progenitor population. In reconstituted mice, Mac-1- CD4- c-kit+ cells gave rise to Mac-1(lo) CD4- cells, which gave rise to Mac-1(lo) CD4(lo) cells. Mac-1- CD4- c-kit+ cells had long-term self-renewal potential, with each cell being capable of giving rise to more than 10(4) functionally similar Mac-1- CD4- c-kit+ cells. At least half of Mac-1(lo) CD4- cells had transient self-renewal potential, detected in the spleen 7 days after reconstitution. Mac-1(lo) CD4(lo) cells did not have detectable self-renewal potential. The identification of a lineage of multipotent progenitors provides an important tool for identifying genes that regulate self-renewal and lineage commitment.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>9169840</pmid><doi>10.1242/dev.124.10.1929</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 1997-05, Vol.124 (10), p.1929-1939 |
| issn | 0950-1991 1477-9129 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79025005 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Bone Marrow Cells Bone Marrow Transplantation CD4 Antigens - analysis Cells, Cultured Hematopoiesis - physiology Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells - chemistry Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - radiation effects Macrophage-1 Antigen - analysis Mice Mice, Inbred C57BL Proto-Oncogene Proteins c-kit - analysis Spleen - cytology Thy-1 Antigens - analysis |
| title | Identification of a lineage of multipotent hematopoietic progenitors |
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