The Mumps Virus Fusion Protein mRNA Sequence and Homology among the Paramyxoviridae Proteins
Department of Virology, School of Medicine, Karolinska Institute, Stockholm S-105 21, Sweden The complete nucleotide sequence of the fusion protein (F) mRNA of the virulent SBL-1 strain of mumps virus has been determined by sequencing cDNA clones and mRNA and confirmed by partially sequencing the ge...
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| Veröffentlicht in: | Journal of general virology 1989-04, Vol.70 (4), p.801-807 |
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| container_title | Journal of general virology |
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| creator | Elango, Narayanasamy Varsanyi, Tamas M Kovamees, Jan Norrby, Erling |
| description | Department of Virology, School of Medicine, Karolinska Institute, Stockholm S-105 21, Sweden
The complete nucleotide sequence of the fusion protein (F) mRNA of the virulent SBL-1 strain of mumps virus has been determined by sequencing cDNA clones and mRNA and confirmed by partially sequencing the genomic RNA. The mRNA was 1721 nucleotides long excluding the poly(A) sequence and had one long open reading frame which encoded a protein of 538 amino acids with a calculated M r of 58791. The predicted amino acid sequence had a proteolytic cleavage/activation site, Arg Arg His Lys Arg, cleavage at which yields proteins F 2 and F 1 . The uncleaved protein contained three highly hydrophobic regions: (i) the amino-terminal signal peptide, (ii) the aminoterminal region of F 1 and (iii) the carboxy-terminal membrane anchorage domain. There were seven potential N -glycosylation sites, two in F 2 and five in F 1 . Comparison of the virulent strain F protein sequence with that of an avirulent strain of mumps virus showed a difference of 14 amino acids. Among paramyxoviruses, mumps virus fusion protein shows the highest degree of homology with the fusion proteins of simian virus 5 and Newcastle disease virus.
Keywords: mumps virus, fusion protein, nucleotide sequence, sequence homology
Present address: Biotechnology Research Institute, National Research Council of Canada, 6100 Avenue Royal Mount, Montreal, Quebec, Canada H4P 2R2.
Received 5 October 1988;
accepted 29 November 1988. |
| doi_str_mv | 10.1099/0022-1317-70-4-801 |
| format | Article |
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The complete nucleotide sequence of the fusion protein (F) mRNA of the virulent SBL-1 strain of mumps virus has been determined by sequencing cDNA clones and mRNA and confirmed by partially sequencing the genomic RNA. The mRNA was 1721 nucleotides long excluding the poly(A) sequence and had one long open reading frame which encoded a protein of 538 amino acids with a calculated M r of 58791. The predicted amino acid sequence had a proteolytic cleavage/activation site, Arg Arg His Lys Arg, cleavage at which yields proteins F 2 and F 1 . The uncleaved protein contained three highly hydrophobic regions: (i) the amino-terminal signal peptide, (ii) the aminoterminal region of F 1 and (iii) the carboxy-terminal membrane anchorage domain. There were seven potential N -glycosylation sites, two in F 2 and five in F 1 . Comparison of the virulent strain F protein sequence with that of an avirulent strain of mumps virus showed a difference of 14 amino acids. Among paramyxoviruses, mumps virus fusion protein shows the highest degree of homology with the fusion proteins of simian virus 5 and Newcastle disease virus.
Keywords: mumps virus, fusion protein, nucleotide sequence, sequence homology
Present address: Biotechnology Research Institute, National Research Council of Canada, 6100 Avenue Royal Mount, Montreal, Quebec, Canada H4P 2R2.
Received 5 October 1988;
accepted 29 November 1988.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-70-4-801</identifier><identifier>PMID: 2732706</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Molecular Sequence Data ; Morphology, structure, chemical composition, physicochemical properties ; Mumps virus - genetics ; Paramyxoviridae - genetics ; RNA, Messenger - genetics ; RNA, Viral - genetics ; Sequence Homology, Nucleic Acid ; Vero Cells ; Viral Fusion Proteins - genetics ; Virology</subject><ispartof>Journal of general virology, 1989-04, Vol.70 (4), p.801-807</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3181-d7a68e33dc03dfc1352b05b4a704b4e962e72e4416036c2fef2ce4a4275604fc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3732,3733,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7339782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2732706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elango, Narayanasamy</creatorcontrib><creatorcontrib>Varsanyi, Tamas M</creatorcontrib><creatorcontrib>Kovamees, Jan</creatorcontrib><creatorcontrib>Norrby, Erling</creatorcontrib><title>The Mumps Virus Fusion Protein mRNA Sequence and Homology among the Paramyxoviridae Proteins</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Department of Virology, School of Medicine, Karolinska Institute, Stockholm S-105 21, Sweden
The complete nucleotide sequence of the fusion protein (F) mRNA of the virulent SBL-1 strain of mumps virus has been determined by sequencing cDNA clones and mRNA and confirmed by partially sequencing the genomic RNA. The mRNA was 1721 nucleotides long excluding the poly(A) sequence and had one long open reading frame which encoded a protein of 538 amino acids with a calculated M r of 58791. The predicted amino acid sequence had a proteolytic cleavage/activation site, Arg Arg His Lys Arg, cleavage at which yields proteins F 2 and F 1 . The uncleaved protein contained three highly hydrophobic regions: (i) the amino-terminal signal peptide, (ii) the aminoterminal region of F 1 and (iii) the carboxy-terminal membrane anchorage domain. There were seven potential N -glycosylation sites, two in F 2 and five in F 1 . Comparison of the virulent strain F protein sequence with that of an avirulent strain of mumps virus showed a difference of 14 amino acids. Among paramyxoviruses, mumps virus fusion protein shows the highest degree of homology with the fusion proteins of simian virus 5 and Newcastle disease virus.
Keywords: mumps virus, fusion protein, nucleotide sequence, sequence homology
Present address: Biotechnology Research Institute, National Research Council of Canada, 6100 Avenue Royal Mount, Montreal, Quebec, Canada H4P 2R2.
Received 5 October 1988;
accepted 29 November 1988.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Mumps virus - genetics</subject><subject>Paramyxoviridae - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Vero Cells</subject><subject>Viral Fusion Proteins - genetics</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1PGzEQhi1EBYH2DyAh-YDKadvxx66zRxRBQaItamlPSJbXO5sYrdfBzrbk3-MoIac5vM87M3oIOWPwhUFdfwXgvGCCqUJBIYspsAMyYbIqC57jQzLZA8fkJKVnACZlqY7IEVeCK6gm5OlxgfT76JeJ_nVxTPRmTC4M9CGGFbqB-l8_ruhvfBlxsEjN0NLb4EMf5mtqfBjmdJX7DyYav34N_1x0rcH3cvpIPnSmT_hpN0_Jn5vrx9ltcf_z293s6r6wgk1Z0SpTTVGI1oJoO8tEyRsoG2kUyEZiXXFUHKVkFYjK8g47blEayVVZgeysOCWft3uXMeRP00p7lyz2vRkwjEmrGrioasgg34I2hpQidnoZnTdxrRnojVK9MaY3xrQCLXVWmkvnu-1j47HdV3YOc36xy02ypu-iGaxLe0wJUaspz9jlFlu4-eK_i6jnOHiXP2lc0Fnd_uAbQrWLjQ</recordid><startdate>198904</startdate><enddate>198904</enddate><creator>Elango, Narayanasamy</creator><creator>Varsanyi, Tamas M</creator><creator>Kovamees, Jan</creator><creator>Norrby, Erling</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198904</creationdate><title>The Mumps Virus Fusion Protein mRNA Sequence and Homology among the Paramyxoviridae Proteins</title><author>Elango, Narayanasamy ; Varsanyi, Tamas M ; Kovamees, Jan ; Norrby, Erling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3181-d7a68e33dc03dfc1352b05b4a704b4e962e72e4416036c2fef2ce4a4275604fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Mumps virus - genetics</topic><topic>Paramyxoviridae - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Vero Cells</topic><topic>Viral Fusion Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elango, Narayanasamy</creatorcontrib><creatorcontrib>Varsanyi, Tamas M</creatorcontrib><creatorcontrib>Kovamees, Jan</creatorcontrib><creatorcontrib>Norrby, Erling</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elango, Narayanasamy</au><au>Varsanyi, Tamas M</au><au>Kovamees, Jan</au><au>Norrby, Erling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Mumps Virus Fusion Protein mRNA Sequence and Homology among the Paramyxoviridae Proteins</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1989-04</date><risdate>1989</risdate><volume>70</volume><issue>4</issue><spage>801</spage><epage>807</epage><pages>801-807</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Department of Virology, School of Medicine, Karolinska Institute, Stockholm S-105 21, Sweden
The complete nucleotide sequence of the fusion protein (F) mRNA of the virulent SBL-1 strain of mumps virus has been determined by sequencing cDNA clones and mRNA and confirmed by partially sequencing the genomic RNA. The mRNA was 1721 nucleotides long excluding the poly(A) sequence and had one long open reading frame which encoded a protein of 538 amino acids with a calculated M r of 58791. The predicted amino acid sequence had a proteolytic cleavage/activation site, Arg Arg His Lys Arg, cleavage at which yields proteins F 2 and F 1 . The uncleaved protein contained three highly hydrophobic regions: (i) the amino-terminal signal peptide, (ii) the aminoterminal region of F 1 and (iii) the carboxy-terminal membrane anchorage domain. There were seven potential N -glycosylation sites, two in F 2 and five in F 1 . Comparison of the virulent strain F protein sequence with that of an avirulent strain of mumps virus showed a difference of 14 amino acids. Among paramyxoviruses, mumps virus fusion protein shows the highest degree of homology with the fusion proteins of simian virus 5 and Newcastle disease virus.
Keywords: mumps virus, fusion protein, nucleotide sequence, sequence homology
Present address: Biotechnology Research Institute, National Research Council of Canada, 6100 Avenue Royal Mount, Montreal, Quebec, Canada H4P 2R2.
Received 5 October 1988;
accepted 29 November 1988.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>2732706</pmid><doi>10.1099/0022-1317-70-4-801</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular Fundamental and applied biological sciences. Psychology Microbiology Molecular Sequence Data Morphology, structure, chemical composition, physicochemical properties Mumps virus - genetics Paramyxoviridae - genetics RNA, Messenger - genetics RNA, Viral - genetics Sequence Homology, Nucleic Acid Vero Cells Viral Fusion Proteins - genetics Virology |
| title | The Mumps Virus Fusion Protein mRNA Sequence and Homology among the Paramyxoviridae Proteins |
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