High-Level Expression in Streptomyces lividans 66 of a Gene Encoding Streptomyces Subtilisin Inhibitor from Streptomyces albogriseolus S-3253

A secretory expression system for Streptomyces subtilisin inhibitor (SSI) was established in a heterologous host, Streptomyces lividans 66, by introducing the 1.8-kbp BgIII/SaII fragment containing SSI gene into the Streptomyces multicopy vector, pIJ 702. The expression of SSI did not depend on the...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 1989-03, Vol.105 (3), p.372-376
Hauptverfasser: Obata, Shusei, Furukubo, Susumu, Kumagai, Izumi, Takahashi, Hideo, Miura, Kin-ichiro
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container_end_page 376
container_issue 3
container_start_page 372
container_title Journal of biochemistry (Tokyo)
container_volume 105
creator Obata, Shusei
Furukubo, Susumu
Kumagai, Izumi
Takahashi, Hideo
Miura, Kin-ichiro
description A secretory expression system for Streptomyces subtilisin inhibitor (SSI) was established in a heterologous host, Streptomyces lividans 66, by introducing the 1.8-kbp BgIII/SaII fragment containing SSI gene into the Streptomyces multicopy vector, pIJ 702. The expression of SSI did not depend on the orientation of the 1.8-kbp BgIII/SaII fragment or on the promoter for tyrosinase gene (mel) in pIJ 702, which suggested that this fragment also carries the SSI promoter. The expressed SSI in S. lividans 66 was secreted into the culture medium in a large amount, as observed with the original strain, S. albogriseolus S-3253. Amino acid sequence analysis showed that the SSI secreted from S. lividans 66 contained three additional amino acid residues in the NH2 -terminal region. The inhibitory activity toward subtilisin BPN’ and the antigenic activity of the SSI secreted from S. lividans 66 were found to be identical with those of authentic SSI.
doi_str_mv 10.1093/oxfordjournals.jbchem.a122671
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subjects Amino Acid Sequence
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Base Sequence
Biological and medical sciences
Biotechnology
Chromatography, DEAE-Cellulose
Chromatography, Gel
DNA, Bacterial - genetics
Electrophoresis, Polyacrylamide Gel
Escherichia coli - enzymology
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular Sequence Data
Plasmids
Streptomyces - genetics
Streptomyces - metabolism
Streptomyces lividans
Transformation, Bacterial
title High-Level Expression in Streptomyces lividans 66 of a Gene Encoding Streptomyces Subtilisin Inhibitor from Streptomyces albogriseolus S-3253
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