High-Level Expression in Streptomyces lividans 66 of a Gene Encoding Streptomyces Subtilisin Inhibitor from Streptomyces albogriseolus S-3253
A secretory expression system for Streptomyces subtilisin inhibitor (SSI) was established in a heterologous host, Streptomyces lividans 66, by introducing the 1.8-kbp BgIII/SaII fragment containing SSI gene into the Streptomyces multicopy vector, pIJ 702. The expression of SSI did not depend on the...
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Veröffentlicht in: | Journal of biochemistry (Tokyo) 1989-03, Vol.105 (3), p.372-376 |
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creator | Obata, Shusei Furukubo, Susumu Kumagai, Izumi Takahashi, Hideo Miura, Kin-ichiro |
description | A secretory expression system for Streptomyces subtilisin inhibitor (SSI) was established in a heterologous host, Streptomyces lividans 66, by introducing the 1.8-kbp BgIII/SaII fragment containing SSI gene into the Streptomyces multicopy vector, pIJ 702. The expression of SSI did not depend on the orientation of the 1.8-kbp BgIII/SaII fragment or on the promoter for tyrosinase gene (mel) in pIJ 702, which suggested that this fragment also carries the SSI promoter. The expressed SSI in S. lividans 66 was secreted into the culture medium in a large amount, as observed with the original strain, S. albogriseolus S-3253. Amino acid sequence analysis showed that the SSI secreted from S. lividans 66 contained three additional amino acid residues in the NH2 -terminal region. The inhibitory activity toward subtilisin BPN’ and the antigenic activity of the SSI secreted from S. lividans 66 were found to be identical with those of authentic SSI. |
doi_str_mv | 10.1093/oxfordjournals.jbchem.a122671 |
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The expression of SSI did not depend on the orientation of the 1.8-kbp BgIII/SaII fragment or on the promoter for tyrosinase gene (mel) in pIJ 702, which suggested that this fragment also carries the SSI promoter. The expressed SSI in S. lividans 66 was secreted into the culture medium in a large amount, as observed with the original strain, S. albogriseolus S-3253. Amino acid sequence analysis showed that the SSI secreted from S. lividans 66 contained three additional amino acid residues in the NH2 -terminal region. The inhibitory activity toward subtilisin BPN’ and the antigenic activity of the SSI secreted from S. lividans 66 were found to be identical with those of authentic SSI.</description><identifier>ISSN: 0021-924X</identifier><identifier>EISSN: 1756-2651</identifier><identifier>DOI: 10.1093/oxfordjournals.jbchem.a122671</identifier><identifier>PMID: 2659584</identifier><identifier>CODEN: JOBIAO</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Chromatography, DEAE-Cellulose ; Chromatography, Gel ; DNA, Bacterial - genetics ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli - enzymology ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Methods. Procedures. Technologies ; Molecular Sequence Data ; Plasmids ; Streptomyces - genetics ; Streptomyces - metabolism ; Streptomyces lividans ; Transformation, Bacterial</subject><ispartof>Journal of biochemistry (Tokyo), 1989-03, Vol.105 (3), p.372-376</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-6a1c2ec8891901890e76bfff41ac2edfe46425c69abf516469035d1e2679f2183</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7245892$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2659584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Obata, Shusei</creatorcontrib><creatorcontrib>Furukubo, Susumu</creatorcontrib><creatorcontrib>Kumagai, Izumi</creatorcontrib><creatorcontrib>Takahashi, Hideo</creatorcontrib><creatorcontrib>Miura, Kin-ichiro</creatorcontrib><title>High-Level Expression in Streptomyces lividans 66 of a Gene Encoding Streptomyces Subtilisin Inhibitor from Streptomyces albogriseolus S-3253</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>A secretory expression system for Streptomyces subtilisin inhibitor (SSI) was established in a heterologous host, Streptomyces lividans 66, by introducing the 1.8-kbp BgIII/SaII fragment containing SSI gene into the Streptomyces multicopy vector, pIJ 702. The expression of SSI did not depend on the orientation of the 1.8-kbp BgIII/SaII fragment or on the promoter for tyrosinase gene (mel) in pIJ 702, which suggested that this fragment also carries the SSI promoter. The expressed SSI in S. lividans 66 was secreted into the culture medium in a large amount, as observed with the original strain, S. albogriseolus S-3253. Amino acid sequence analysis showed that the SSI secreted from S. lividans 66 contained three additional amino acid residues in the NH2 -terminal region. The inhibitory activity toward subtilisin BPN’ and the antigenic activity of the SSI secreted from S. lividans 66 were found to be identical with those of authentic SSI.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromatography, DEAE-Cellulose</subject><subject>Chromatography, Gel</subject><subject>DNA, Bacterial - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces lividans</subject><subject>Transformation, Bacterial</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFuEzEQhi0EKqHwCEg-ALcNttf2rg8cUBWaQgRCKVLFxfJ67cRh107t3Sp9CN4ZV1lFyonTyPN__4w8PwDvMZpjJMqP4WBDbHdhjF51ab5r9Nb0c4UJ4RV-Bma4YrwgnOHnYIYQwYUg9O4leJXS7ulJyvICXGRdsJrOwN-l22yLlXkwHVwc9tGk5IKHzsP1EM1-CP2jNgl27sG1yifIOQwWKnhtvIELr0Pr_OacXY_N4DqX8owbv3WNG0KENob-HFNdEzbRJRO6MZuKkrDyNXhh86fMm6legl9fFrdXy2L14_rm6vOq0JRWQ8EV1sTouhZYIFwLZCreWGspVrnfWkM5JUxzoRrLMKdcoJK12OQLCUtwXV6CD8e5-xjuR5MG2bukTdcpb8KYZCXypShH_wUxwxXhQmTw0xHUMaQUjZX76HoVHyVG8ik3eZ6bPOYmp9yy_-20aGx6057cU1BZfzfpKmnV2ai8dumEVYSyWpCMFUfMpcEcTrKKfySvyorJ5d1v-ZN_Rd_Wt1R-L_8BP5u5ww</recordid><startdate>19890301</startdate><enddate>19890301</enddate><creator>Obata, Shusei</creator><creator>Furukubo, Susumu</creator><creator>Kumagai, Izumi</creator><creator>Takahashi, Hideo</creator><creator>Miura, Kin-ichiro</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19890301</creationdate><title>High-Level Expression in Streptomyces lividans 66 of a Gene Encoding Streptomyces Subtilisin Inhibitor from Streptomyces albogriseolus S-3253</title><author>Obata, Shusei ; Furukubo, Susumu ; Kumagai, Izumi ; Takahashi, Hideo ; Miura, Kin-ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-6a1c2ec8891901890e76bfff41ac2edfe46425c69abf516469035d1e2679f2183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chromatography, DEAE-Cellulose</topic><topic>Chromatography, Gel</topic><topic>DNA, Bacterial - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Escherichia coli - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces lividans</topic><topic>Transformation, Bacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Obata, Shusei</creatorcontrib><creatorcontrib>Furukubo, Susumu</creatorcontrib><creatorcontrib>Kumagai, Izumi</creatorcontrib><creatorcontrib>Takahashi, Hideo</creatorcontrib><creatorcontrib>Miura, Kin-ichiro</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Obata, Shusei</au><au>Furukubo, Susumu</au><au>Kumagai, Izumi</au><au>Takahashi, Hideo</au><au>Miura, Kin-ichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Level Expression in Streptomyces lividans 66 of a Gene Encoding Streptomyces Subtilisin Inhibitor from Streptomyces albogriseolus S-3253</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1989-03-01</date><risdate>1989</risdate><volume>105</volume><issue>3</issue><spage>372</spage><epage>376</epage><pages>372-376</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><coden>JOBIAO</coden><abstract>A secretory expression system for Streptomyces subtilisin inhibitor (SSI) was established in a heterologous host, Streptomyces lividans 66, by introducing the 1.8-kbp BgIII/SaII fragment containing SSI gene into the Streptomyces multicopy vector, pIJ 702. The expression of SSI did not depend on the orientation of the 1.8-kbp BgIII/SaII fragment or on the promoter for tyrosinase gene (mel) in pIJ 702, which suggested that this fragment also carries the SSI promoter. The expressed SSI in S. lividans 66 was secreted into the culture medium in a large amount, as observed with the original strain, S. albogriseolus S-3253. Amino acid sequence analysis showed that the SSI secreted from S. lividans 66 contained three additional amino acid residues in the NH2 -terminal region. The inhibitory activity toward subtilisin BPN’ and the antigenic activity of the SSI secreted from S. lividans 66 were found to be identical with those of authentic SSI.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2659584</pmid><doi>10.1093/oxfordjournals.jbchem.a122671</doi><tpages>5</tpages></addata></record> |
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subjects | Amino Acid Sequence Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Base Sequence Biological and medical sciences Biotechnology Chromatography, DEAE-Cellulose Chromatography, Gel DNA, Bacterial - genetics Electrophoresis, Polyacrylamide Gel Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic engineering Genetic technics Methods. Procedures. Technologies Molecular Sequence Data Plasmids Streptomyces - genetics Streptomyces - metabolism Streptomyces lividans Transformation, Bacterial |
title | High-Level Expression in Streptomyces lividans 66 of a Gene Encoding Streptomyces Subtilisin Inhibitor from Streptomyces albogriseolus S-3253 |
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