Modulatory actions of serotonin on ionic conductances of hippocampal dentate granule cells
Pressure ejection of serotonin (2 × 10 4M) onto dentate granule neurons in vitro produced a short-lasting membrane hyperpolarization associated with a 10–30% decrease in the input resistance. The hyperpolarization magnitude depended on the extracellular K + concentration but not on the extra or intr...
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| Veröffentlicht in: | Neuroscience 1989, Vol.29 (2), p.443-451 |
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| creator | Baskys, A. C.E., Niesen Davies, M.F. Carlen, P.L. |
| description | Pressure ejection of serotonin (2 × 10
4M) onto dentate granule neurons
in vitro produced a short-lasting membrane hyperpolarization associated with a 10–30% decrease in the input resistance. The hyperpolarization magnitude depended on the extracellular K
+ concentration but not on the extra or intracellular Ca
2+ concentration. It was followed by a depolarization, especially when serotonin was applied onto the perisomatic area of the neuron.
The post-spike-train afterhyperpolarization, which represents a Ca
2+-dependent K
+ conductance, was decreased by serotonin by 10–100% and remained reduced for 2–10 min following the serotonin-induced hyperpolarization. Decreased adaptation of cell firing was also observed following serotonin application. Ca
2+ action potentials evoked by intracellular depolarizing current pulses in the presence of the Na
+ channel blocker tetrodotoxin and the K
+ channel blocker tetraethylammonium were followed by a large afterhyperpolarization. which was markedly reduced for several minutes following serotonin application. The preceding Ca
2+ action potential was either unaffected or prolonged.
The hyperpolarization occurring in response to localized application of serotonin, and the reduction of the afterhyperpolarization, may represent two different mechanisms of serotonin action, probably mediated by different mechanisms. The slow time course of the late depolarization and the after-hyperpolarization depression represent modulatory effects of serotonin on dentate granule neurons. |
| doi_str_mv | 10.1016/0306-4522(89)90071-7 |
| format | Article |
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4M) onto dentate granule neurons
in vitro produced a short-lasting membrane hyperpolarization associated with a 10–30% decrease in the input resistance. The hyperpolarization magnitude depended on the extracellular K
+ concentration but not on the extra or intracellular Ca
2+ concentration. It was followed by a depolarization, especially when serotonin was applied onto the perisomatic area of the neuron.
The post-spike-train afterhyperpolarization, which represents a Ca
2+-dependent K
+ conductance, was decreased by serotonin by 10–100% and remained reduced for 2–10 min following the serotonin-induced hyperpolarization. Decreased adaptation of cell firing was also observed following serotonin application. Ca
2+ action potentials evoked by intracellular depolarizing current pulses in the presence of the Na
+ channel blocker tetrodotoxin and the K
+ channel blocker tetraethylammonium were followed by a large afterhyperpolarization. which was markedly reduced for several minutes following serotonin application. The preceding Ca
2+ action potential was either unaffected or prolonged.
The hyperpolarization occurring in response to localized application of serotonin, and the reduction of the afterhyperpolarization, may represent two different mechanisms of serotonin action, probably mediated by different mechanisms. The slow time course of the late depolarization and the after-hyperpolarization depression represent modulatory effects of serotonin on dentate granule neurons.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/0306-4522(89)90071-7</identifier><identifier>PMID: 2471114</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>5-HT ; ACSF ; Action Potentials - drug effects ; afterhyperpolarization ; AHP ; Animals ; artificial cerebrospinal fluid ; Biological and medical sciences ; Calcium - physiology ; Central nervous system ; dentate gyrus ; EGTA ; Electrophysiology ; EPSP/IPSP ; ethyleneglycolbis (aminoethylether)tetra-acetate ; excitatory/inhibitory postsynaptic potential ; Fundamental and applied biological sciences. Psychology ; granule cells ; hippocampus ; Hippocampus - drug effects ; Hippocampus - physiology ; In Vitro Techniques ; input resistance ; Ion Channels - drug effects ; Ion Channels - physiology ; ions ; Male ; Membrane Potentials - drug effects ; R in ; Rats ; Rats, Inbred F344 ; resting membrane potential ; RMP ; serotonin ; Serotonin - pharmacology ; TEA ; tetraethylammonium chloride ; tetrodotoxin ; TTX ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 1989, Vol.29 (2), p.443-451</ispartof><rights>1989 IBRO</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-54bde512639301f049a5aa0d9bbfd21f46e2fbadaa6c61ca95fc349938adc1913</citedby><cites>FETCH-LOGICAL-c484t-54bde512639301f049a5aa0d9bbfd21f46e2fbadaa6c61ca95fc349938adc1913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0306452289900717$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19389080$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2471114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baskys, A.</creatorcontrib><creatorcontrib>C.E., Niesen</creatorcontrib><creatorcontrib>Davies, M.F.</creatorcontrib><creatorcontrib>Carlen, P.L.</creatorcontrib><title>Modulatory actions of serotonin on ionic conductances of hippocampal dentate granule cells</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Pressure ejection of serotonin (2 × 10
4M) onto dentate granule neurons
in vitro produced a short-lasting membrane hyperpolarization associated with a 10–30% decrease in the input resistance. The hyperpolarization magnitude depended on the extracellular K
+ concentration but not on the extra or intracellular Ca
2+ concentration. It was followed by a depolarization, especially when serotonin was applied onto the perisomatic area of the neuron.
The post-spike-train afterhyperpolarization, which represents a Ca
2+-dependent K
+ conductance, was decreased by serotonin by 10–100% and remained reduced for 2–10 min following the serotonin-induced hyperpolarization. Decreased adaptation of cell firing was also observed following serotonin application. Ca
2+ action potentials evoked by intracellular depolarizing current pulses in the presence of the Na
+ channel blocker tetrodotoxin and the K
+ channel blocker tetraethylammonium were followed by a large afterhyperpolarization. which was markedly reduced for several minutes following serotonin application. The preceding Ca
2+ action potential was either unaffected or prolonged.
The hyperpolarization occurring in response to localized application of serotonin, and the reduction of the afterhyperpolarization, may represent two different mechanisms of serotonin action, probably mediated by different mechanisms. The slow time course of the late depolarization and the after-hyperpolarization depression represent modulatory effects of serotonin on dentate granule neurons.</description><subject>5-HT</subject><subject>ACSF</subject><subject>Action Potentials - drug effects</subject><subject>afterhyperpolarization</subject><subject>AHP</subject><subject>Animals</subject><subject>artificial cerebrospinal fluid</subject><subject>Biological and medical sciences</subject><subject>Calcium - physiology</subject><subject>Central nervous system</subject><subject>dentate gyrus</subject><subject>EGTA</subject><subject>Electrophysiology</subject><subject>EPSP/IPSP</subject><subject>ethyleneglycolbis (aminoethylether)tetra-acetate</subject><subject>excitatory/inhibitory postsynaptic potential</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>granule cells</subject><subject>hippocampus</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - physiology</subject><subject>In Vitro Techniques</subject><subject>input resistance</subject><subject>Ion Channels - drug effects</subject><subject>Ion Channels - physiology</subject><subject>ions</subject><subject>Male</subject><subject>Membrane Potentials - drug effects</subject><subject>R in</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>resting membrane potential</subject><subject>RMP</subject><subject>serotonin</subject><subject>Serotonin - pharmacology</subject><subject>TEA</subject><subject>tetraethylammonium chloride</subject><subject>tetrodotoxin</subject><subject>TTX</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1TAQRi0EKreFNwDJGxAsUjyxE8cbJFRRilTUDWzYWBP_gFGuHWwHqW9P0ntVdtQbS57jb2YOIS-AnQOD_h3jrG9E17ZvBvVWMSahkY_IDgbJG9kJ8Zjs7pGn5LSUX2w9neAn5KQVEgDEjnz_kuwyYU35lqKpIcVCk6fF5VRTDJGmSNfHYKhJ0S6mYjTuDvkZ5jkZ3M84Uetixeroj4xxmRw1bprKM_LE41Tc8-N9Rr5dfvx6cdVc33z6fPHhujFiELXpxGhdB23PFWfgmVDYITKrxtHbFrzoXetHtIi96cGg6rzhQik-oDWggJ-R14fcOaffiytV70PZJsDo0lK0VAyUBPkgCB0HaCVbQXEATU6lZOf1nMMe860Gpjf3ehOrN7F6UPrOvd7yXx7zl3Hv7P2no-y1_upYx2Jw8qssE8q_7HUlxYat_fsD51Zrf4LLupjgVu82ZGeqtin8f5C_drahHg</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Baskys, A.</creator><creator>C.E., Niesen</creator><creator>Davies, M.F.</creator><creator>Carlen, P.L.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Modulatory actions of serotonin on ionic conductances of hippocampal dentate granule cells</title><author>Baskys, A. ; C.E., Niesen ; Davies, M.F. ; Carlen, P.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-54bde512639301f049a5aa0d9bbfd21f46e2fbadaa6c61ca95fc349938adc1913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>5-HT</topic><topic>ACSF</topic><topic>Action Potentials - drug effects</topic><topic>afterhyperpolarization</topic><topic>AHP</topic><topic>Animals</topic><topic>artificial cerebrospinal fluid</topic><topic>Biological and medical sciences</topic><topic>Calcium - physiology</topic><topic>Central nervous system</topic><topic>dentate gyrus</topic><topic>EGTA</topic><topic>Electrophysiology</topic><topic>EPSP/IPSP</topic><topic>ethyleneglycolbis (aminoethylether)tetra-acetate</topic><topic>excitatory/inhibitory postsynaptic potential</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>granule cells</topic><topic>hippocampus</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - physiology</topic><topic>In Vitro Techniques</topic><topic>input resistance</topic><topic>Ion Channels - drug effects</topic><topic>Ion Channels - physiology</topic><topic>ions</topic><topic>Male</topic><topic>Membrane Potentials - drug effects</topic><topic>R in</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>resting membrane potential</topic><topic>RMP</topic><topic>serotonin</topic><topic>Serotonin - pharmacology</topic><topic>TEA</topic><topic>tetraethylammonium chloride</topic><topic>tetrodotoxin</topic><topic>TTX</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baskys, A.</creatorcontrib><creatorcontrib>C.E., Niesen</creatorcontrib><creatorcontrib>Davies, M.F.</creatorcontrib><creatorcontrib>Carlen, P.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baskys, A.</au><au>C.E., Niesen</au><au>Davies, M.F.</au><au>Carlen, P.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulatory actions of serotonin on ionic conductances of hippocampal dentate granule cells</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1989</date><risdate>1989</risdate><volume>29</volume><issue>2</issue><spage>443</spage><epage>451</epage><pages>443-451</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Pressure ejection of serotonin (2 × 10
4M) onto dentate granule neurons
in vitro produced a short-lasting membrane hyperpolarization associated with a 10–30% decrease in the input resistance. The hyperpolarization magnitude depended on the extracellular K
+ concentration but not on the extra or intracellular Ca
2+ concentration. It was followed by a depolarization, especially when serotonin was applied onto the perisomatic area of the neuron.
The post-spike-train afterhyperpolarization, which represents a Ca
2+-dependent K
+ conductance, was decreased by serotonin by 10–100% and remained reduced for 2–10 min following the serotonin-induced hyperpolarization. Decreased adaptation of cell firing was also observed following serotonin application. Ca
2+ action potentials evoked by intracellular depolarizing current pulses in the presence of the Na
+ channel blocker tetrodotoxin and the K
+ channel blocker tetraethylammonium were followed by a large afterhyperpolarization. which was markedly reduced for several minutes following serotonin application. The preceding Ca
2+ action potential was either unaffected or prolonged.
The hyperpolarization occurring in response to localized application of serotonin, and the reduction of the afterhyperpolarization, may represent two different mechanisms of serotonin action, probably mediated by different mechanisms. The slow time course of the late depolarization and the after-hyperpolarization depression represent modulatory effects of serotonin on dentate granule neurons.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2471114</pmid><doi>10.1016/0306-4522(89)90071-7</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Neuroscience, 1989, Vol.29 (2), p.443-451 |
| issn | 0306-4522 1873-7544 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79019717 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 5-HT ACSF Action Potentials - drug effects afterhyperpolarization AHP Animals artificial cerebrospinal fluid Biological and medical sciences Calcium - physiology Central nervous system dentate gyrus EGTA Electrophysiology EPSP/IPSP ethyleneglycolbis (aminoethylether)tetra-acetate excitatory/inhibitory postsynaptic potential Fundamental and applied biological sciences. Psychology granule cells hippocampus Hippocampus - drug effects Hippocampus - physiology In Vitro Techniques input resistance Ion Channels - drug effects Ion Channels - physiology ions Male Membrane Potentials - drug effects R in Rats Rats, Inbred F344 resting membrane potential RMP serotonin Serotonin - pharmacology TEA tetraethylammonium chloride tetrodotoxin TTX Vertebrates: nervous system and sense organs |
| title | Modulatory actions of serotonin on ionic conductances of hippocampal dentate granule cells |
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