Crystallization of a synthetic analog of calcium-binding site III of rabbit skeletal troponin C
Single crystals of the calcium-binding peptide AcA98STnC (90–123)amide, which is a synthetic fragment analog of rabbit skeletal troponin C calcium-binding site III, have been grown in the tetragonal crystal system. Crystals were developed to a maximum size of 0.60 × 0.60 × 0.75-mm by the technique o...
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| Veröffentlicht in: | The Journal of biological chemistry 1989-06, Vol.264 (17), p.10261-10263 |
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| container_end_page | 10263 |
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| container_issue | 17 |
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| container_title | The Journal of biological chemistry |
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| creator | Delbaere, L T J Vandonselaar, M Reid, R E |
| description | Single crystals of the calcium-binding peptide AcA98STnC (90–123)amide, which is a synthetic fragment analog of rabbit skeletal troponin C calcium-binding site III, have been grown in the tetragonal crystal system. Crystals were developed to a maximum size of 0.60 × 0.60 × 0.75-mm by the technique of washing and reseeding. The space group is I41 with a = 40.21(1) Å, c = 34.07(1) Å; there is 1 polypeptide fragment/crystallographic asymmetric unit, and the solvent content is estimated to be 29%. The crystals diffract to at least 1.8-Å d spacings and are stable in the x-ray beam for 1 week. |
| doi_str_mv | 10.1016/S0021-9258(18)81793-8 |
| format | Article |
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| ispartof | The Journal of biological chemistry, 1989-06, Vol.264 (17), p.10261-10263 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Analytical, structural and metabolic biochemistry Animals Binding and carrier proteins Binding Sites Biological and medical sciences Calcium - metabolism Crystallization Fundamental and applied biological sciences. Psychology Muscles - metabolism Peptides - isolation & purification Peptides - metabolism Protein Binding Protein Conformation Proteins Rabbits skeletal muscle Troponin - metabolism Troponin C X-Ray Diffraction |
| title | Crystallization of a synthetic analog of calcium-binding site III of rabbit skeletal troponin C |
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