Nuclear factor E2F mediates basic transcription and trans-activation by E1a of the human MYC promoter
Transcription from one of the two initiation sites, P1 and P2, of the dual human MYC promoter seems to be essential in all proliferating cells. To identify proteins and target structures for MYC regulation, a DNA region was analyzed that is critical for P2 promoter activity. Here, we show that a nuc...
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| Veröffentlicht in: | Genes & development 1989-04, Vol.3 (4), p.527-536 |
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| creator | THALMEIER, K SYNOVZIK, H MERTZ, R WINNACKER, E.-L LIPP, M |
| description | Transcription from one of the two initiation sites, P1 and P2, of the dual human MYC promoter seems to be essential in all proliferating cells. To identify proteins and target structures for MYC regulation, a DNA region was analyzed that is critical for P2 promoter activity. Here, we show that a nuclear factor binds to a DNA element within P2, which is conserved perfectly between mouse and man and displays a striking homology to the E1a-inducible E2 promoter of adenovirus type 5 (Ad5). We demonstrate that the same transcription factor, defined recently as E2F, which plays an essential role in the activation of adenovirus early promoters and enhancers, also interacts as a dominant nuclear factor with the MYC promoter. The presence of an intact E2F binding site is required for basic expression and for trans-activation of the P2 promoter by E1a proteins. The human MYC promoter is the first cellular target described for E2F. The results suggest that expression of MYC might be regulated via modulation of E2F by cellular 'E1a-like' factors. |
| doi_str_mv | 10.1101/gad.3.4.527 |
| format | Article |
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To identify proteins and target structures for MYC regulation, a DNA region was analyzed that is critical for P2 promoter activity. Here, we show that a nuclear factor binds to a DNA element within P2, which is conserved perfectly between mouse and man and displays a striking homology to the E1a-inducible E2 promoter of adenovirus type 5 (Ad5). We demonstrate that the same transcription factor, defined recently as E2F, which plays an essential role in the activation of adenovirus early promoters and enhancers, also interacts as a dominant nuclear factor with the MYC promoter. The presence of an intact E2F binding site is required for basic expression and for trans-activation of the P2 promoter by E1a proteins. The human MYC promoter is the first cellular target described for E2F. The results suggest that expression of MYC might be regulated via modulation of E2F by cellular 'E1a-like' factors.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.3.4.527</identifier><identifier>PMID: 2721961</identifier><identifier>CODEN: GEDEEP</identifier><language>eng</language><publisher>Cold Spring Harbor, NY: Cold Spring Harbor Laboratory</publisher><subject>Base Sequence ; Binding Sites ; Biological and medical sciences ; CCAAT-Enhancer-Binding Proteins ; Cell Line ; Chloramphenicol O-Acetyltransferase - metabolism ; DNA-Binding Proteins - genetics ; Enhancer Elements, Genetic ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; HeLa Cells ; Humans ; Methylation ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; NFI Transcription Factors ; Nuclear Proteins ; Nucleotide Mapping ; Oligonucleotide Probes ; Plasmids ; Promoter Regions, Genetic ; Transcription Factors ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. 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To identify proteins and target structures for MYC regulation, a DNA region was analyzed that is critical for P2 promoter activity. Here, we show that a nuclear factor binds to a DNA element within P2, which is conserved perfectly between mouse and man and displays a striking homology to the E1a-inducible E2 promoter of adenovirus type 5 (Ad5). We demonstrate that the same transcription factor, defined recently as E2F, which plays an essential role in the activation of adenovirus early promoters and enhancers, also interacts as a dominant nuclear factor with the MYC promoter. The presence of an intact E2F binding site is required for basic expression and for trans-activation of the P2 promoter by E1a proteins. The human MYC promoter is the first cellular target described for E2F. The results suggest that expression of MYC might be regulated via modulation of E2F by cellular 'E1a-like' factors.</description><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>CCAAT-Enhancer-Binding Proteins</subject><subject>Cell Line</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Methylation</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>NFI Transcription Factors</subject><subject>Nuclear Proteins</subject><subject>Nucleotide Mapping</subject><subject>Oligonucleotide Probes</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Transcription Factors</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Gene Expression Regulation</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Methylation</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>NFI Transcription Factors</topic><topic>Nuclear Proteins</topic><topic>Nucleotide Mapping</topic><topic>Oligonucleotide Probes</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Transcription Factors</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transfection</topic><topic>Y-Box-Binding Protein 1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>THALMEIER, K</creatorcontrib><creatorcontrib>SYNOVZIK, H</creatorcontrib><creatorcontrib>MERTZ, R</creatorcontrib><creatorcontrib>WINNACKER, E.-L</creatorcontrib><creatorcontrib>LIPP, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>THALMEIER, K</au><au>SYNOVZIK, H</au><au>MERTZ, R</au><au>WINNACKER, E.-L</au><au>LIPP, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear factor E2F mediates basic transcription and trans-activation by E1a of the human MYC promoter</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1989-04-01</date><risdate>1989</risdate><volume>3</volume><issue>4</issue><spage>527</spage><epage>536</epage><pages>527-536</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><coden>GEDEEP</coden><abstract>Transcription from one of the two initiation sites, P1 and P2, of the dual human MYC promoter seems to be essential in all proliferating cells. To identify proteins and target structures for MYC regulation, a DNA region was analyzed that is critical for P2 promoter activity. Here, we show that a nuclear factor binds to a DNA element within P2, which is conserved perfectly between mouse and man and displays a striking homology to the E1a-inducible E2 promoter of adenovirus type 5 (Ad5). We demonstrate that the same transcription factor, defined recently as E2F, which plays an essential role in the activation of adenovirus early promoters and enhancers, also interacts as a dominant nuclear factor with the MYC promoter. The presence of an intact E2F binding site is required for basic expression and for trans-activation of the P2 promoter by E1a proteins. The human MYC promoter is the first cellular target described for E2F. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Base Sequence Binding Sites Biological and medical sciences CCAAT-Enhancer-Binding Proteins Cell Line Chloramphenicol O-Acetyltransferase - metabolism DNA-Binding Proteins - genetics Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Gene Expression Regulation HeLa Cells Humans Methylation Molecular and cellular biology Molecular genetics Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Nucleotide Mapping Oligonucleotide Probes Plasmids Promoter Regions, Genetic Transcription Factors Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transfection Y-Box-Binding Protein 1 |
| title | Nuclear factor E2F mediates basic transcription and trans-activation by E1a of the human MYC promoter |
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