Molecular Cloning and Comparative Analysis of the Rhesus Macaque Costimulatory Molecules CD80 (B7-1) and CD86 (B7-2)
To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model, we cloned and sequenced the CD80 (B7-1) and CD86 (B7-2) costimulatory molecules from rhesus macaques. Rhesus CD80 and CD86 were highly homologous to their human counterparts, with overall amino acid homologies...
Gespeichert in:
| Veröffentlicht in: | Cellular immunology 1997-04, Vol.177 (1), p.9-17 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 17 |
|---|---|
| container_issue | 1 |
| container_start_page | 9 |
| container_title | Cellular immunology |
| container_volume | 177 |
| creator | Zhang, Da Johnson, R.Paul |
| description | To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model, we cloned and sequenced the CD80 (B7-1) and CD86 (B7-2) costimulatory molecules from rhesus macaques. Rhesus CD80 and CD86 were highly homologous to their human counterparts, with overall amino acid homologies of greater than 90%, and were specifically recognized by murine antihuman CD80 or CD86 monoclonal antibodies. Stable cell lines expressing rhesus CD80 or CD86 induced proliferation of suboptimally activated CD4+T cells and transcription of cytokine mRNA. Both CD80 and CD86 were able to provide costimulation for interferon-gamma and IL-2 synthesis by rhesus CD4+T cells, but CD80 costimulation also resulted in synthesis of IL-4 and IL-10. The high degree of homology between the rhesus and the human CD80 and CD86 molecules should facilitate analysis of therapeutic interventions directed at this costimulatory pathway in nonhuman primates. |
| doi_str_mv | 10.1006/cimm.1997.1098 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79004135</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0008874997910984</els_id><sourcerecordid>79004135</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-864c106829f8d01be2f2b851f5d4890c7d09469f98ee201a1f9ac2946676b6943</originalsourceid><addsrcrecordid>eNqFkU1r3DAQhkVJSTdpr70FdArJwdsZ2ytLx8T5KuxSKO1ZaOVxomJbW8le2H8fbXfJreQ0zMwzD0IvY18R5gggvlnX93NUqkqtkh_YLBXIchTFCZsBgMxkVapP7CzGPwCIpYJTdqqwBFA4Y-PKd2SnzgRed35wwzM3Q8Nr329MMKPbEr8ZTLeLLnLf8vGF-M8XilPkK2PN34kSGkfXJ8Pow44fdRR5fSeBX91WGV4flHdS_Ovz68_sY2u6SF-O9Zz9frj_VT9lyx-P3-ubZWaLCsZMitIiCJmrVjaAa8rbfC0X2C6aUiqwVQOqFKpVkigHNNgqY_M0EpVYC1UW5-zy4N0En54aR927aKnrzEB-irpSACUWi3dBFJAXi6pI4PwA2uBjDNTqTXC9CTuNoPd56H0eep-H3ueRDi6O5mndU_OGHwNIe3nYU_qHraOgo3U0WGpcIDvqxrv_qV8BvTqWCg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16023573</pqid></control><display><type>article</type><title>Molecular Cloning and Comparative Analysis of the Rhesus Macaque Costimulatory Molecules CD80 (B7-1) and CD86 (B7-2)</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Zhang, Da ; Johnson, R.Paul</creator><creatorcontrib>Zhang, Da ; Johnson, R.Paul</creatorcontrib><description>To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model, we cloned and sequenced the CD80 (B7-1) and CD86 (B7-2) costimulatory molecules from rhesus macaques. Rhesus CD80 and CD86 were highly homologous to their human counterparts, with overall amino acid homologies of greater than 90%, and were specifically recognized by murine antihuman CD80 or CD86 monoclonal antibodies. Stable cell lines expressing rhesus CD80 or CD86 induced proliferation of suboptimally activated CD4+T cells and transcription of cytokine mRNA. Both CD80 and CD86 were able to provide costimulation for interferon-gamma and IL-2 synthesis by rhesus CD4+T cells, but CD80 costimulation also resulted in synthesis of IL-4 and IL-10. The high degree of homology between the rhesus and the human CD80 and CD86 molecules should facilitate analysis of therapeutic interventions directed at this costimulatory pathway in nonhuman primates.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1006/cimm.1997.1098</identifier><identifier>PMID: 9140091</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - immunology ; Antigens, CD - analysis ; Antigens, CD - genetics ; Antigens, CD - physiology ; B7-1 Antigen - analysis ; B7-1 Antigen - genetics ; B7-1 Antigen - physiology ; B7-2 Antigen ; CHO Cells ; Cloning, Molecular ; Cricetinae ; Cytokines - biosynthesis ; Humans ; Macaca mulatta ; Membrane Glycoproteins - analysis ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - physiology ; Molecular Sequence Data</subject><ispartof>Cellular immunology, 1997-04, Vol.177 (1), p.9-17</ispartof><rights>1997 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-864c106829f8d01be2f2b851f5d4890c7d09469f98ee201a1f9ac2946676b6943</citedby><cites>FETCH-LOGICAL-c370t-864c106829f8d01be2f2b851f5d4890c7d09469f98ee201a1f9ac2946676b6943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/cimm.1997.1098$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9140091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Da</creatorcontrib><creatorcontrib>Johnson, R.Paul</creatorcontrib><title>Molecular Cloning and Comparative Analysis of the Rhesus Macaque Costimulatory Molecules CD80 (B7-1) and CD86 (B7-2)</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model, we cloned and sequenced the CD80 (B7-1) and CD86 (B7-2) costimulatory molecules from rhesus macaques. Rhesus CD80 and CD86 were highly homologous to their human counterparts, with overall amino acid homologies of greater than 90%, and were specifically recognized by murine antihuman CD80 or CD86 monoclonal antibodies. Stable cell lines expressing rhesus CD80 or CD86 induced proliferation of suboptimally activated CD4+T cells and transcription of cytokine mRNA. Both CD80 and CD86 were able to provide costimulation for interferon-gamma and IL-2 synthesis by rhesus CD4+T cells, but CD80 costimulation also resulted in synthesis of IL-4 and IL-10. The high degree of homology between the rhesus and the human CD80 and CD86 molecules should facilitate analysis of therapeutic interventions directed at this costimulatory pathway in nonhuman primates.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - physiology</subject><subject>B7-1 Antigen - analysis</subject><subject>B7-1 Antigen - genetics</subject><subject>B7-1 Antigen - physiology</subject><subject>B7-2 Antigen</subject><subject>CHO Cells</subject><subject>Cloning, Molecular</subject><subject>Cricetinae</subject><subject>Cytokines - biosynthesis</subject><subject>Humans</subject><subject>Macaca mulatta</subject><subject>Membrane Glycoproteins - analysis</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - physiology</subject><subject>Molecular Sequence Data</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVJSTdpr70FdArJwdsZ2ytLx8T5KuxSKO1ZaOVxomJbW8le2H8fbXfJreQ0zMwzD0IvY18R5gggvlnX93NUqkqtkh_YLBXIchTFCZsBgMxkVapP7CzGPwCIpYJTdqqwBFA4Y-PKd2SnzgRed35wwzM3Q8Nr329MMKPbEr8ZTLeLLnLf8vGF-M8XilPkK2PN34kSGkfXJ8Pow44fdRR5fSeBX91WGV4flHdS_Ovz68_sY2u6SF-O9Zz9frj_VT9lyx-P3-ubZWaLCsZMitIiCJmrVjaAa8rbfC0X2C6aUiqwVQOqFKpVkigHNNgqY_M0EpVYC1UW5-zy4N0En54aR927aKnrzEB-irpSACUWi3dBFJAXi6pI4PwA2uBjDNTqTXC9CTuNoPd56H0eep-H3ueRDi6O5mndU_OGHwNIe3nYU_qHraOgo3U0WGpcIDvqxrv_qV8BvTqWCg</recordid><startdate>19970410</startdate><enddate>19970410</enddate><creator>Zhang, Da</creator><creator>Johnson, R.Paul</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19970410</creationdate><title>Molecular Cloning and Comparative Analysis of the Rhesus Macaque Costimulatory Molecules CD80 (B7-1) and CD86 (B7-2)</title><author>Zhang, Da ; Johnson, R.Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-864c106829f8d01be2f2b851f5d4890c7d09469f98ee201a1f9ac2946676b6943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - physiology</topic><topic>B7-1 Antigen - analysis</topic><topic>B7-1 Antigen - genetics</topic><topic>B7-1 Antigen - physiology</topic><topic>B7-2 Antigen</topic><topic>CHO Cells</topic><topic>Cloning, Molecular</topic><topic>Cricetinae</topic><topic>Cytokines - biosynthesis</topic><topic>Humans</topic><topic>Macaca mulatta</topic><topic>Membrane Glycoproteins - analysis</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - physiology</topic><topic>Molecular Sequence Data</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Da</creatorcontrib><creatorcontrib>Johnson, R.Paul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Da</au><au>Johnson, R.Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning and Comparative Analysis of the Rhesus Macaque Costimulatory Molecules CD80 (B7-1) and CD86 (B7-2)</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1997-04-10</date><risdate>1997</risdate><volume>177</volume><issue>1</issue><spage>9</spage><epage>17</epage><pages>9-17</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><abstract>To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model, we cloned and sequenced the CD80 (B7-1) and CD86 (B7-2) costimulatory molecules from rhesus macaques. Rhesus CD80 and CD86 were highly homologous to their human counterparts, with overall amino acid homologies of greater than 90%, and were specifically recognized by murine antihuman CD80 or CD86 monoclonal antibodies. Stable cell lines expressing rhesus CD80 or CD86 induced proliferation of suboptimally activated CD4+T cells and transcription of cytokine mRNA. Both CD80 and CD86 were able to provide costimulation for interferon-gamma and IL-2 synthesis by rhesus CD4+T cells, but CD80 costimulation also resulted in synthesis of IL-4 and IL-10. The high degree of homology between the rhesus and the human CD80 and CD86 molecules should facilitate analysis of therapeutic interventions directed at this costimulatory pathway in nonhuman primates.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>9140091</pmid><doi>10.1006/cimm.1997.1098</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-8749 |
| ispartof | Cellular immunology, 1997-04, Vol.177 (1), p.9-17 |
| issn | 0008-8749 1090-2163 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79004135 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Antigens, CD - analysis Antigens, CD - genetics Antigens, CD - physiology B7-1 Antigen - analysis B7-1 Antigen - genetics B7-1 Antigen - physiology B7-2 Antigen CHO Cells Cloning, Molecular Cricetinae Cytokines - biosynthesis Humans Macaca mulatta Membrane Glycoproteins - analysis Membrane Glycoproteins - genetics Membrane Glycoproteins - physiology Molecular Sequence Data |
| title | Molecular Cloning and Comparative Analysis of the Rhesus Macaque Costimulatory Molecules CD80 (B7-1) and CD86 (B7-2) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T23%3A52%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Cloning%20and%20Comparative%20Analysis%20of%20the%20Rhesus%20Macaque%20Costimulatory%20Molecules%20CD80%20(B7-1)%20and%20CD86%20(B7-2)&rft.jtitle=Cellular%20immunology&rft.au=Zhang,%20Da&rft.date=1997-04-10&rft.volume=177&rft.issue=1&rft.spage=9&rft.epage=17&rft.pages=9-17&rft.issn=0008-8749&rft.eissn=1090-2163&rft_id=info:doi/10.1006/cimm.1997.1098&rft_dat=%3Cproquest_cross%3E79004135%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16023573&rft_id=info:pmid/9140091&rft_els_id=S0008874997910984&rfr_iscdi=true |