A three-step procedure for the purification of human basophils from buffy coat blood

We report a method for basophil purification from buffy coats, which avoids positive selection of the cells and gives rise to good purity, yield and functional integrity of the cells. Buffy coat blood (concentrated leukocyte fraction derived from 450 ml venipuncture donations) obtained from healthy...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Inflammation research 1997-04, Vol.46 (4), p.137-142
Hauptverfasser:	Gibbs, B F, Noll, T, Falcone, F H, Haas, H, Vollmer, E, Vollrath, I, Wolff, H H, Amon, U
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigen-Antibody Reactions Antigens, CD19 - immunology Antigens, CD19 - isolation & purification Basophils - cytology Basophils - drug effects Basophils - immunology Blood Donors Calcimycin - pharmacology CD2 Antigens - immunology CD2 Antigens - isolation & purification Cell Separation - methods Centrifugation - methods Centrifugation, Density Gradient Histamine Release - drug effects Humans Immunoglobulin E - immunology Immunomagnetic Separation Ionophores - pharmacology Leukocytes - cytology Lipopolysaccharide Receptors - immunology Lipopolysaccharide Receptors - isolation & purification Receptors, IgG - immunology Receptors, IgG - isolation & purification Staining and Labeling
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!