Ultrastructural localization of zinc ions in the rat prostate: An autometallographic study
BACKGROUND The prostate contains high amounts of free zinc ions which are excreted into the seminal fluid. The extra‐ and intracellular distribution of zinc ions using the highly specific autometallographical (AMG) method is described. METHODS Prostates from sulfide‐perfused rats were excised, and t...
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| Veröffentlicht in: | The Prostate 1997-05, Vol.31 (2), p.125-130 |
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| container_title | The Prostate |
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| creator | Sorensen, M.B. Stoltenberg, M. Juhl, S. Danscher, G. Ernst, E. |
| description | BACKGROUND
The prostate contains high amounts of free zinc ions which are excreted into the seminal fluid. The extra‐ and intracellular distribution of zinc ions using the highly specific autometallographical (AMG) method is described.
METHODS
Prostates from sulfide‐perfused rats were excised, and the ZnS crystals were silver‐enhanced to sizes detectable by the electron and light microscope.
RESULTS
AMGZnS grains were found primarily in the acinic lumen of the lateral lobes. The dorsal lobe stained only faintly, while the ventral lobe was void of grains. At ultrastructural levels, the presence of zinc ions was confined to apical secretory vesicles and lysosome‐like structure of the epithelium of mainly the lateral lobes.
CONCLUSIONS
We suggest a constant secretion of zinc ions from the epithelial cells into both the acinar lumen and the intercellular canaliculi, and that the zinc enriched secretory cells in the prostate belong to a system of glandular cells that uses zinc ions to aggregate macromolecules to be excreted. Prostate 31:125–130, 1997. © 1997 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1097-0045(19970501)31:2<125::AID-PROS8>3.0.CO;2-M |
| format | Article |
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The prostate contains high amounts of free zinc ions which are excreted into the seminal fluid. The extra‐ and intracellular distribution of zinc ions using the highly specific autometallographical (AMG) method is described.
METHODS
Prostates from sulfide‐perfused rats were excised, and the ZnS crystals were silver‐enhanced to sizes detectable by the electron and light microscope.
RESULTS
AMGZnS grains were found primarily in the acinic lumen of the lateral lobes. The dorsal lobe stained only faintly, while the ventral lobe was void of grains. At ultrastructural levels, the presence of zinc ions was confined to apical secretory vesicles and lysosome‐like structure of the epithelium of mainly the lateral lobes.
CONCLUSIONS
We suggest a constant secretion of zinc ions from the epithelial cells into both the acinar lumen and the intercellular canaliculi, and that the zinc enriched secretory cells in the prostate belong to a system of glandular cells that uses zinc ions to aggregate macromolecules to be excreted. Prostate 31:125–130, 1997. © 1997 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/(SICI)1097-0045(19970501)31:2<125::AID-PROS8>3.0.CO;2-M</identifier><identifier>PMID: 9140126</identifier><identifier>CODEN: PRSTDS</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; autometallography ; Biological and medical sciences ; chelatable zinc ; Fundamental and applied biological sciences. Psychology ; Male ; Metallothionein - analysis ; Microscopy, Electron ; Prostate - chemistry ; Prostate - ultrastructure ; Rats ; Rats, Wistar ; secretory cells ; silver sulphide method ; Vertebrates: urinary system ; Zinc - analysis ; zinc ions</subject><ispartof>The Prostate, 1997-05, Vol.31 (2), p.125-130</ispartof><rights>Copyright © 1997 Wiley‐Liss, Inc.</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4348-1fc648572bfb5979afec77010312377e0b4b3631380b672d561404309eae49fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-0045%2819970501%2931%3A2%3C125%3A%3AAID-PROS8%3E3.0.CO%3B2-M$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-0045%2819970501%2931%3A2%3C125%3A%3AAID-PROS8%3E3.0.CO%3B2-M$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2662106$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9140126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sorensen, M.B.</creatorcontrib><creatorcontrib>Stoltenberg, M.</creatorcontrib><creatorcontrib>Juhl, S.</creatorcontrib><creatorcontrib>Danscher, G.</creatorcontrib><creatorcontrib>Ernst, E.</creatorcontrib><title>Ultrastructural localization of zinc ions in the rat prostate: An autometallographic study</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
The prostate contains high amounts of free zinc ions which are excreted into the seminal fluid. The extra‐ and intracellular distribution of zinc ions using the highly specific autometallographical (AMG) method is described.
METHODS
Prostates from sulfide‐perfused rats were excised, and the ZnS crystals were silver‐enhanced to sizes detectable by the electron and light microscope.
RESULTS
AMGZnS grains were found primarily in the acinic lumen of the lateral lobes. The dorsal lobe stained only faintly, while the ventral lobe was void of grains. At ultrastructural levels, the presence of zinc ions was confined to apical secretory vesicles and lysosome‐like structure of the epithelium of mainly the lateral lobes.
CONCLUSIONS
We suggest a constant secretion of zinc ions from the epithelial cells into both the acinar lumen and the intercellular canaliculi, and that the zinc enriched secretory cells in the prostate belong to a system of glandular cells that uses zinc ions to aggregate macromolecules to be excreted. Prostate 31:125–130, 1997. © 1997 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>autometallography</subject><subject>Biological and medical sciences</subject><subject>chelatable zinc</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Metallothionein - analysis</subject><subject>Microscopy, Electron</subject><subject>Prostate - chemistry</subject><subject>Prostate - ultrastructure</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>secretory cells</subject><subject>silver sulphide method</subject><subject>Vertebrates: urinary system</subject><subject>Zinc - analysis</subject><subject>zinc ions</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkNFu0zAUhiMEGmXwCEi-QGi7SDm2kzgpaFMVoC3a6MQYQ9wcOamzBdyk2I6ge3ocUnoDElc-Ouf3r09fEJxSGFMA9uLocpEvjilkIgSI4iOaZQJioMecTtgryuLJZLp4HV58WF6mJ3wM43z5koXn94LR_s_9YARMQBhRLh4Gj6z9CuC7gR0EBxmNgLJkFHy50s5I60xXus5ITXRbSl3fSVe3DWkrclc3JfGzJXVD3K0iRjqyMa110qkJmTZEdq5dKye1bm-M3NzWJbGuW20fBw8qqa16snsPg6u3bz7m8_BsOVvk07OwjHiUhrQqkyiNBSuqIs5EJitVCuFJOWVcCAVFVPCEU55CkQi2ihPPHnHIlFRRVkl-GDwfej3V905Zh-valkpr2ai2syjSLEvjGHzwegiWHt8aVeHG1GtptkgBe-uIvXXsDWJvEP9YR06RobeO6K3jb-vIETBf-v25b366Q-iKtVrte3ea_f3Z7i6t11sZ2ZS13cdYkjAKfezzEPtRa7X9i-6_cP9iGxa-Ohyqa-vUz321NN8wEVzEeP1-hu9mn_KLOZsj5b8AcN-5vg</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>Sorensen, M.B.</creator><creator>Stoltenberg, M.</creator><creator>Juhl, S.</creator><creator>Danscher, G.</creator><creator>Ernst, E.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970501</creationdate><title>Ultrastructural localization of zinc ions in the rat prostate: An autometallographic study</title><author>Sorensen, M.B. ; Stoltenberg, M. ; Juhl, S. ; Danscher, G. ; Ernst, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4348-1fc648572bfb5979afec77010312377e0b4b3631380b672d561404309eae49fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>autometallography</topic><topic>Biological and medical sciences</topic><topic>chelatable zinc</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Metallothionein - analysis</topic><topic>Microscopy, Electron</topic><topic>Prostate - chemistry</topic><topic>Prostate - ultrastructure</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>secretory cells</topic><topic>silver sulphide method</topic><topic>Vertebrates: urinary system</topic><topic>Zinc - analysis</topic><topic>zinc ions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sorensen, M.B.</creatorcontrib><creatorcontrib>Stoltenberg, M.</creatorcontrib><creatorcontrib>Juhl, S.</creatorcontrib><creatorcontrib>Danscher, G.</creatorcontrib><creatorcontrib>Ernst, E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sorensen, M.B.</au><au>Stoltenberg, M.</au><au>Juhl, S.</au><au>Danscher, G.</au><au>Ernst, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural localization of zinc ions in the rat prostate: An autometallographic study</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>31</volume><issue>2</issue><spage>125</spage><epage>130</epage><pages>125-130</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><coden>PRSTDS</coden><abstract>BACKGROUND
The prostate contains high amounts of free zinc ions which are excreted into the seminal fluid. The extra‐ and intracellular distribution of zinc ions using the highly specific autometallographical (AMG) method is described.
METHODS
Prostates from sulfide‐perfused rats were excised, and the ZnS crystals were silver‐enhanced to sizes detectable by the electron and light microscope.
RESULTS
AMGZnS grains were found primarily in the acinic lumen of the lateral lobes. The dorsal lobe stained only faintly, while the ventral lobe was void of grains. At ultrastructural levels, the presence of zinc ions was confined to apical secretory vesicles and lysosome‐like structure of the epithelium of mainly the lateral lobes.
CONCLUSIONS
We suggest a constant secretion of zinc ions from the epithelial cells into both the acinar lumen and the intercellular canaliculi, and that the zinc enriched secretory cells in the prostate belong to a system of glandular cells that uses zinc ions to aggregate macromolecules to be excreted. Prostate 31:125–130, 1997. © 1997 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>9140126</pmid><doi>10.1002/(SICI)1097-0045(19970501)31:2<125::AID-PROS8>3.0.CO;2-M</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals autometallography Biological and medical sciences chelatable zinc Fundamental and applied biological sciences. Psychology Male Metallothionein - analysis Microscopy, Electron Prostate - chemistry Prostate - ultrastructure Rats Rats, Wistar secretory cells silver sulphide method Vertebrates: urinary system Zinc - analysis zinc ions |
| title | Ultrastructural localization of zinc ions in the rat prostate: An autometallographic study |
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