Fat-storing (Ito) cells of rat liver synthesize and secrete apolipoproteins: comparison with hepatocytes
Fat-storing cells of the liver store most of the vitamin A of the body. Vitamin A is present in a few large fat droplets within the cell. The aim of our study was to investigate apolipoprotein biosynthesis in isolated fat-storing cells from rat liver during the time in culture. Isolated rat hepatocy...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 1989-07, Vol.97 (1), p.163-172 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
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| creator | Ramadori, G. Rieder, H. Theiss, F. zum Büschenfelde, K-H.Meyer |
| description | Fat-storing cells of the liver store most of the vitamin A of the body. Vitamin A is present in a few large fat droplets within the cell. The aim of our study was to investigate apolipoprotein biosynthesis in isolated fat-storing cells from rat liver during the time in culture. Isolated rat hepatocytes were studied for comparison. Proteins were biosynthetically labeled and further identified by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Apoproteins in culture supernatants were identified by density gradient ultracentrifugation and by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of aliquots of the gradient. Rat plasma was processed in the same way and used for comparison. Fat-storing cells synthesized and secreted apoprotein E, apoprotein A-I, apoprotein A-IV, and low amounts of apoprotein C. The synthesis of these proteins increased during the culture time, reaching a maximum at the fifth day after isolation. The proteins were identified mostly as apoproteins of high-density lipoprotein. Hepatocytes synthesized and secreted apoproteins of all classes of lipoproteins. The distribution of high-density lipoprotein apoproteins was similar to that of fat-storing cells but hepatocytes produced larger amounts of the apoproteins. |
| doi_str_mv | 10.1016/0016-5085(89)91431-5 |
| format | Article |
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Vitamin A is present in a few large fat droplets within the cell. The aim of our study was to investigate apolipoprotein biosynthesis in isolated fat-storing cells from rat liver during the time in culture. Isolated rat hepatocytes were studied for comparison. Proteins were biosynthetically labeled and further identified by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Apoproteins in culture supernatants were identified by density gradient ultracentrifugation and by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of aliquots of the gradient. Rat plasma was processed in the same way and used for comparison. Fat-storing cells synthesized and secreted apoprotein E, apoprotein A-I, apoprotein A-IV, and low amounts of apoprotein C. The synthesis of these proteins increased during the culture time, reaching a maximum at the fifth day after isolation. 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Vitamin A is present in a few large fat droplets within the cell. The aim of our study was to investigate apolipoprotein biosynthesis in isolated fat-storing cells from rat liver during the time in culture. Isolated rat hepatocytes were studied for comparison. Proteins were biosynthetically labeled and further identified by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Apoproteins in culture supernatants were identified by density gradient ultracentrifugation and by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of aliquots of the gradient. Rat plasma was processed in the same way and used for comparison. Fat-storing cells synthesized and secreted apoprotein E, apoprotein A-I, apoprotein A-IV, and low amounts of apoprotein C. The synthesis of these proteins increased during the culture time, reaching a maximum at the fifth day after isolation. The proteins were identified mostly as apoproteins of high-density lipoprotein. Hepatocytes synthesized and secreted apoproteins of all classes of lipoproteins. The distribution of high-density lipoprotein apoproteins was similar to that of fat-storing cells but hepatocytes produced larger amounts of the apoproteins.</description><subject>Animals</subject><subject>Apolipoproteins - analysis</subject><subject>Apolipoproteins - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>Centrifugation, Density Gradient</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipid Metabolism</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Liver. Bile. Biliary tracts</subject><subject>Precipitin Tests</subject><subject>Rats</subject><subject>Rats, Inbred BN</subject><subject>Vertebrates: digestive system</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFO3DAQhi0Egi3lDVrJB4TgEGo7cWL3UKlCpSAhceFueZ1x11U2Dh4v1fbpcbqrPfZij_R_Mx5_hHzi7JYz3n5h5agkU_Ja6RvNm5pX8ogsuBSqKpk4JosDckY-IP5mjOla8VNyKjrBVdstyOre5gpzTGH8Ra8fc7yhDoYBafQ02UyH8AaJ4nbMK8DwF6gde4rgEuRST3EIU5xSzBBG_EpdXE82BYwj_RPyiq5gsjm6bQb8SE68HRAu9vc5ebn_8XL3UD09_3y8-_5UuaYWuWq5a530she67j3z3gknlK6hq7njjWaldksumfBLL5UW0lqta9ktm66RbX1OrnZjy1KvG8Bs1gHnH9kR4gZNp7RWUjQFbHagSxExgTdTCmubtoYzM_s1szwzyzNKm39-jSxtn_fzN8s19IemvdCSX-5zi84OPtnRBTxgbSu6Vs2vf9thUFS8BUgGXYDRQR8SuGz6GP6_xzu7-Zcu</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Ramadori, G.</creator><creator>Rieder, H.</creator><creator>Theiss, F.</creator><creator>zum Büschenfelde, K-H.Meyer</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890701</creationdate><title>Fat-storing (Ito) cells of rat liver synthesize and secrete apolipoproteins: comparison with hepatocytes</title><author>Ramadori, G. ; Rieder, H. ; Theiss, F. ; zum Büschenfelde, K-H.Meyer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-61c6c5f5d293df0ffc2c2893e731c1490893cb1502fbf58925aa99357b474563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Apolipoproteins - analysis</topic><topic>Apolipoproteins - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>Centrifugation, Density Gradient</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lipid Metabolism</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Liver. Bile. Biliary tracts</topic><topic>Precipitin Tests</topic><topic>Rats</topic><topic>Rats, Inbred BN</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramadori, G.</creatorcontrib><creatorcontrib>Rieder, H.</creatorcontrib><creatorcontrib>Theiss, F.</creatorcontrib><creatorcontrib>zum Büschenfelde, K-H.Meyer</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramadori, G.</au><au>Rieder, H.</au><au>Theiss, F.</au><au>zum Büschenfelde, K-H.Meyer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fat-storing (Ito) cells of rat liver synthesize and secrete apolipoproteins: comparison with hepatocytes</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>97</volume><issue>1</issue><spage>163</spage><epage>172</epage><pages>163-172</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><coden>GASTAB</coden><abstract>Fat-storing cells of the liver store most of the vitamin A of the body. Vitamin A is present in a few large fat droplets within the cell. The aim of our study was to investigate apolipoprotein biosynthesis in isolated fat-storing cells from rat liver during the time in culture. Isolated rat hepatocytes were studied for comparison. Proteins were biosynthetically labeled and further identified by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Apoproteins in culture supernatants were identified by density gradient ultracentrifugation and by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of aliquots of the gradient. Rat plasma was processed in the same way and used for comparison. Fat-storing cells synthesized and secreted apoprotein E, apoprotein A-I, apoprotein A-IV, and low amounts of apoprotein C. The synthesis of these proteins increased during the culture time, reaching a maximum at the fifth day after isolation. 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| subjects | Animals Apolipoproteins - analysis Apolipoproteins - biosynthesis Biological and medical sciences Cell Separation Cells, Cultured Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Female Fundamental and applied biological sciences. Psychology Lipid Metabolism Liver - cytology Liver - metabolism Liver. Bile. Biliary tracts Precipitin Tests Rats Rats, Inbred BN Vertebrates: digestive system |
| title | Fat-storing (Ito) cells of rat liver synthesize and secrete apolipoproteins: comparison with hepatocytes |
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