Size-exclusion chromatography of protected synthetic polypeptide tandems in an organic solvent
A novel method for analytical and preparative size exclusion chromatography of large water‐insoluble protected peptides in an organic solvent was developed. This method was applied to analysis and separation of protected synthetic peptide tandem repeats and to a control of the reaction of peptide fr...
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| Veröffentlicht in: | The journal of peptide research 1997-03, Vol.49 (3), p.232-239 |
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| container_end_page | 239 |
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| container_title | The journal of peptide research |
| container_volume | 49 |
| creator | KARNOUP, ANTON S. SHIRYAEV, VIACHESLAV M. MEDVEDKIN, VIACHESLAV N. MITIN, YURY V. |
| description | A novel method for analytical and preparative size exclusion chromatography of large water‐insoluble protected peptides in an organic solvent was developed. This method was applied to analysis and separation of protected synthetic peptide tandem repeats and to a control of the reaction of peptide fragment coupling. Columns containing Toyopearl HW‐40, HW‐50, HW‐55 and HW‐60 gels of Fine grade were used, and the selectivity of each sorbent, as well as the Chromatographie behaviour of the peptides on them, were examined. Separation ranges of these gels applied to separation of protected peptides in DMF were shown to be much smaller (ca. 400–14000 Da) than those of the same gels applied to protein separations in water buffers (100–1000000 Da). An initial evaluation of efficiency of Toyopearl HW Superfine grade gels with respect to similar separations was performed. © Munksgaard 1997. |
| doi_str_mv | 10.1111/j.1399-3011.1997.tb00882.x |
| format | Article |
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This method was applied to analysis and separation of protected synthetic peptide tandem repeats and to a control of the reaction of peptide fragment coupling. Columns containing Toyopearl HW‐40, HW‐50, HW‐55 and HW‐60 gels of Fine grade were used, and the selectivity of each sorbent, as well as the Chromatographie behaviour of the peptides on them, were examined. Separation ranges of these gels applied to separation of protected peptides in DMF were shown to be much smaller (ca. 400–14000 Da) than those of the same gels applied to protein separations in water buffers (100–1000000 Da). An initial evaluation of efficiency of Toyopearl HW Superfine grade gels with respect to similar separations was performed. © Munksgaard 1997.</description><subject>Amino Acids - chemistry</subject><subject>Calibration</subject><subject>Chromatography, Gel - methods</subject><subject>Dimethyl Sulfoxide</subject><subject>Dimethylformamide</subject><subject>Fluorenes - chemistry</subject><subject>Fmoc-strategy</subject><subject>fragment condensation: gel filtration: tandem repeats</subject><subject>Molecular Weight</subject><subject>Peptides - analysis</subject><subject>Peptides - isolation & purification</subject><subject>Polymers - chemistry</subject><subject>protected peptides</subject><subject>Solubility</subject><subject>Solvents</subject><subject>Toyopearl HW</subject><issn>1397-002X</issn><issn>1399-3011</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkU1v0zAYxy0EGqPwEZAiDtwS_FInNhfEOjaQOjgMxE5Yrv14dUniYLus4dOT0qp3fLGl_8vz6GeEXhFckem82VSESVkyTEhFpGyqvMJYCFrtHqHzk_T437spMaZ3T9GzlDYYE0ZZfYbOJOGE8voc_bj1f6CEnWm3yYe-MOsYOp3DfdTDeiyCK4YYMpgMtkhjn9eQvSmG0I4DDNlbKLLuLXSp8H2h-yLEe91PjhTa39Dn5-iJ022CF8d7hr5dffi6-Fguv1x_WrxflmaOBS2NFY3klhtZa-OoE0LiOTdCMMYxw05TbInWznAqHTZCOlmDBWronKyYnbMZen3onbb9tYWUVeeTgbbVPYRtUo2QEteYTca3B6OJIaUITg3RdzqOimC1h6s2ak9Q7QmqPVx1hKt2U_jlccp21YE9RY80J_3dQX_wLYz_0awWF5eXdPqbGSoPDT5l2J0adPyp6oY1XH3_fK3uOLm5wctbtWR_ATIrm2k</recordid><startdate>199703</startdate><enddate>199703</enddate><creator>KARNOUP, ANTON S.</creator><creator>SHIRYAEV, VIACHESLAV M.</creator><creator>MEDVEDKIN, VIACHESLAV N.</creator><creator>MITIN, YURY V.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199703</creationdate><title>Size-exclusion chromatography of protected synthetic polypeptide tandems in an organic solvent</title><author>KARNOUP, ANTON S. ; SHIRYAEV, VIACHESLAV M. ; MEDVEDKIN, VIACHESLAV N. ; MITIN, YURY V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4082-cd8795d5c96acf2f889045c88335030fa20d1aafc529f0c89f96ede2c241b3d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acids - chemistry</topic><topic>Calibration</topic><topic>Chromatography, Gel - methods</topic><topic>Dimethyl Sulfoxide</topic><topic>Dimethylformamide</topic><topic>Fluorenes - chemistry</topic><topic>Fmoc-strategy</topic><topic>fragment condensation: gel filtration: tandem repeats</topic><topic>Molecular Weight</topic><topic>Peptides - analysis</topic><topic>Peptides - isolation & purification</topic><topic>Polymers - chemistry</topic><topic>protected peptides</topic><topic>Solubility</topic><topic>Solvents</topic><topic>Toyopearl HW</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KARNOUP, ANTON S.</creatorcontrib><creatorcontrib>SHIRYAEV, VIACHESLAV M.</creatorcontrib><creatorcontrib>MEDVEDKIN, VIACHESLAV N.</creatorcontrib><creatorcontrib>MITIN, YURY V.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of peptide research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KARNOUP, ANTON S.</au><au>SHIRYAEV, VIACHESLAV M.</au><au>MEDVEDKIN, VIACHESLAV N.</au><au>MITIN, YURY V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Size-exclusion chromatography of protected synthetic polypeptide tandems in an organic solvent</atitle><jtitle>The journal of peptide research</jtitle><addtitle>J Pept Res</addtitle><date>1997-03</date><risdate>1997</risdate><volume>49</volume><issue>3</issue><spage>232</spage><epage>239</epage><pages>232-239</pages><issn>1397-002X</issn><eissn>1399-3011</eissn><abstract>A novel method for analytical and preparative size exclusion chromatography of large water‐insoluble protected peptides in an organic solvent was developed. This method was applied to analysis and separation of protected synthetic peptide tandem repeats and to a control of the reaction of peptide fragment coupling. Columns containing Toyopearl HW‐40, HW‐50, HW‐55 and HW‐60 gels of Fine grade were used, and the selectivity of each sorbent, as well as the Chromatographie behaviour of the peptides on them, were examined. Separation ranges of these gels applied to separation of protected peptides in DMF were shown to be much smaller (ca. 400–14000 Da) than those of the same gels applied to protein separations in water buffers (100–1000000 Da). An initial evaluation of efficiency of Toyopearl HW Superfine grade gels with respect to similar separations was performed. © Munksgaard 1997.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9151256</pmid><doi>10.1111/j.1399-3011.1997.tb00882.x</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1397-002X |
| ispartof | The journal of peptide research, 1997-03, Vol.49 (3), p.232-239 |
| issn | 1397-002X 1399-3011 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acids - chemistry Calibration Chromatography, Gel - methods Dimethyl Sulfoxide Dimethylformamide Fluorenes - chemistry Fmoc-strategy fragment condensation: gel filtration: tandem repeats Molecular Weight Peptides - analysis Peptides - isolation & purification Polymers - chemistry protected peptides Solubility Solvents Toyopearl HW |
| title | Size-exclusion chromatography of protected synthetic polypeptide tandems in an organic solvent |
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