Endothelin immunoreactivity in medium from cultured porcine aortic endothelial cells correlates with the biological activity
Using the radioimmunoassay (RIA) of endothelin (ET), we measured immunoreactive ET (IR-ET) in culture medium of porcine aortic endothelial cells. The immunoreactivity in the medium was compared with the biological activity. The amount of IR-ET released into the medium was calculated at 250–350 pg/10...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1989-04, Vol.160 (2), p.602-608 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Matsumura, Yasuo Ikegawa, Ruriko Ohyama, Tadashi Hayashi, Kazutaka Shiro, Morimoto |
| description | Using the radioimmunoassay (RIA) of endothelin (ET), we measured immunoreactive ET (IR-ET) in culture medium of porcine aortic endothelial cells. The immunoreactivity in the medium was compared with the biological activity. The amount of IR-ET released into the medium was calculated at 250–350 pg/10
6 cells/hr. The amount of IR-ET released into the culture medium increased progressively with 3–24 hr of incubation and corresponded to the increase in medium-induced vasoconstriction of rat isolated aorta. When the vasoconstrictor activities in the culture medium were plotted against the IR-ET concentration determined by RIA, the concentration-response curve showed similarity to that obtained with synthetic porcine ET. This RIA system will be a useful for investigating mechanisms of ET secretion from endothelial cells. |
| doi_str_mv | 10.1016/0006-291X(89)92475-3 |
| format | Article |
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6 cells/hr. The amount of IR-ET released into the culture medium increased progressively with 3–24 hr of incubation and corresponded to the increase in medium-induced vasoconstriction of rat isolated aorta. When the vasoconstrictor activities in the culture medium were plotted against the IR-ET concentration determined by RIA, the concentration-response curve showed similarity to that obtained with synthetic porcine ET. This RIA system will be a useful for investigating mechanisms of ET secretion from endothelial cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(89)92475-3</identifier><identifier>PMID: 2655593</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Aminoacids, peptides. Hormones. Neuropeptides ; Analytical, structural and metabolic biochemistry ; Animals ; aorta ; Aorta, Thoracic ; Biological and medical sciences ; Cells, Cultured ; Culture Media ; Endothelins ; Endothelium, Vascular - analysis ; Endothelium, Vascular - physiology ; Fundamental and applied biological sciences. Psychology ; Male ; Muscle Contraction ; Peptides - analysis ; Peptides - physiology ; Peptides - standards ; pigs ; Proteins ; Radioimmunoassay - methods ; Radioimmunoassay - standards ; Rats ; Rats, Inbred Strains ; Swine ; Vasoconstrictor Agents - analysis</subject><ispartof>Biochemical and biophysical research communications, 1989-04, Vol.160 (2), p.602-608</ispartof><rights>1989 Academic Press, Inc.</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-73d274eb732643fd957161ee128e07a0c3f9832f9d602f9aa652dfd4a54a0ae63</citedby><cites>FETCH-LOGICAL-c417t-73d274eb732643fd957161ee128e07a0c3f9832f9d602f9aa652dfd4a54a0ae63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X89924753$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6795747$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2655593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsumura, Yasuo</creatorcontrib><creatorcontrib>Ikegawa, Ruriko</creatorcontrib><creatorcontrib>Ohyama, Tadashi</creatorcontrib><creatorcontrib>Hayashi, Kazutaka</creatorcontrib><creatorcontrib>Shiro, Morimoto</creatorcontrib><title>Endothelin immunoreactivity in medium from cultured porcine aortic endothelial cells correlates with the biological activity</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Using the radioimmunoassay (RIA) of endothelin (ET), we measured immunoreactive ET (IR-ET) in culture medium of porcine aortic endothelial cells. The immunoreactivity in the medium was compared with the biological activity. The amount of IR-ET released into the medium was calculated at 250–350 pg/10
6 cells/hr. The amount of IR-ET released into the culture medium increased progressively with 3–24 hr of incubation and corresponded to the increase in medium-induced vasoconstriction of rat isolated aorta. When the vasoconstrictor activities in the culture medium were plotted against the IR-ET concentration determined by RIA, the concentration-response curve showed similarity to that obtained with synthetic porcine ET. This RIA system will be a useful for investigating mechanisms of ET secretion from endothelial cells.</description><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>aorta</subject><subject>Aorta, Thoracic</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Endothelins</subject><subject>Endothelium, Vascular - analysis</subject><subject>Endothelium, Vascular - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Muscle Contraction</subject><subject>Peptides - analysis</subject><subject>Peptides - physiology</subject><subject>Peptides - standards</subject><subject>pigs</subject><subject>Proteins</subject><subject>Radioimmunoassay - methods</subject><subject>Radioimmunoassay - standards</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Swine</subject><subject>Vasoconstrictor Agents - analysis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFTEYhYNY2mv1HyhkIWIXo_maZLIRpNS2UHDTgruQm7xjIzOT2yTTUvDHm_He3mW7SSDneT9yDkLvKflCCZVfCSGyYZr--tzpE82Eahv-Cq0o0aRhlIjXaLVHjtCbnP8QQqmQ-hAdMtm2reYr9Pds8rHcwhAmHMZxnmIC60q4D-UR17cRfJhH3Kc4YjcPZU7g8SYmFybANqYSHIanFnbADoYhYxdTgsEWyPghlFtcVbwOcYi_g6vQ04C36KC3Q4Z3u_sY3fw4uz69aK5-nl-efr9qnKCqNIp7pgSsFWdS8N7rVlFJASjrgChLHO91x1mvvST1tFa2zPde2FZYYkHyY_Rp23eT4t0MuZgx5GVTO0Gcs1Gd7lRH1YsgbTmpk3QFxRZ0KeacoDebFEabHg0lZknHLNabxXrTafM_HcNr2Ydd_3ldnd0X7eKo-sedbnM1qk92ciHvManq18Wy5rctBtW0-wDJZBdgcjWrBK4YH8Pze_wDHSaucg</recordid><startdate>19890428</startdate><enddate>19890428</enddate><creator>Matsumura, Yasuo</creator><creator>Ikegawa, Ruriko</creator><creator>Ohyama, Tadashi</creator><creator>Hayashi, Kazutaka</creator><creator>Shiro, Morimoto</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19890428</creationdate><title>Endothelin immunoreactivity in medium from cultured porcine aortic endothelial cells correlates with the biological activity</title><author>Matsumura, Yasuo ; Ikegawa, Ruriko ; Ohyama, Tadashi ; Hayashi, Kazutaka ; Shiro, Morimoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-73d274eb732643fd957161ee128e07a0c3f9832f9d602f9aa652dfd4a54a0ae63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>aorta</topic><topic>Aorta, Thoracic</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Endothelins</topic><topic>Endothelium, Vascular - analysis</topic><topic>Endothelium, Vascular - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Muscle Contraction</topic><topic>Peptides - analysis</topic><topic>Peptides - physiology</topic><topic>Peptides - standards</topic><topic>pigs</topic><topic>Proteins</topic><topic>Radioimmunoassay - methods</topic><topic>Radioimmunoassay - standards</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Swine</topic><topic>Vasoconstrictor Agents - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsumura, Yasuo</creatorcontrib><creatorcontrib>Ikegawa, Ruriko</creatorcontrib><creatorcontrib>Ohyama, Tadashi</creatorcontrib><creatorcontrib>Hayashi, Kazutaka</creatorcontrib><creatorcontrib>Shiro, Morimoto</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsumura, Yasuo</au><au>Ikegawa, Ruriko</au><au>Ohyama, Tadashi</au><au>Hayashi, Kazutaka</au><au>Shiro, Morimoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelin immunoreactivity in medium from cultured porcine aortic endothelial cells correlates with the biological activity</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1989-04-28</date><risdate>1989</risdate><volume>160</volume><issue>2</issue><spage>602</spage><epage>608</epage><pages>602-608</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Using the radioimmunoassay (RIA) of endothelin (ET), we measured immunoreactive ET (IR-ET) in culture medium of porcine aortic endothelial cells. The immunoreactivity in the medium was compared with the biological activity. The amount of IR-ET released into the medium was calculated at 250–350 pg/10
6 cells/hr. The amount of IR-ET released into the culture medium increased progressively with 3–24 hr of incubation and corresponded to the increase in medium-induced vasoconstriction of rat isolated aorta. When the vasoconstrictor activities in the culture medium were plotted against the IR-ET concentration determined by RIA, the concentration-response curve showed similarity to that obtained with synthetic porcine ET. This RIA system will be a useful for investigating mechanisms of ET secretion from endothelial cells.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2655593</pmid><doi>10.1016/0006-291X(89)92475-3</doi><tpages>7</tpages></addata></record> |
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| subjects | Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals aorta Aorta, Thoracic Biological and medical sciences Cells, Cultured Culture Media Endothelins Endothelium, Vascular - analysis Endothelium, Vascular - physiology Fundamental and applied biological sciences. Psychology Male Muscle Contraction Peptides - analysis Peptides - physiology Peptides - standards pigs Proteins Radioimmunoassay - methods Radioimmunoassay - standards Rats Rats, Inbred Strains Swine Vasoconstrictor Agents - analysis |
| title | Endothelin immunoreactivity in medium from cultured porcine aortic endothelial cells correlates with the biological activity |
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