Detection of two isotypically different antibodies produced by a murine hybridoma

A hybridoma, F31P46B, secreting monoclonal antibodies (mAbs) comprised of mu and gamma heavy chains in association with a single kappa light chain, has been characterized. This hybridoma was prepared by fusing splenocytes, derived from a BALB/c mouse immunized with Vibrio vulnificus and SP2/O-Ag-14...

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Veröffentlicht in:	Hybridoma 1997-04, Vol.16 (2), p.195-199
Hauptverfasser:	CHEN, D, ALTMANN, K, HANNA, P. J, TAMMER, A, UNDERWOOD, J. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - biosynthesis Antibodies, immunoglobulins Antibody Specificity Biological and medical sciences Fundamental and applied biological sciences. Psychology Fundamental immunology Hybridomas - immunology Immunoglobulin G - biosynthesis Immunoglobulin gamma-Chains - biosynthesis Immunoglobulin Heavy Chains - genetics Immunoglobulin kappa-Chains - biosynthesis Immunoglobulin Light Chains - genetics Immunoglobulin M - biosynthesis Immunoglobulin mu-Chains - biosynthesis Immunoglobulin Variable Region - genetics Mice Mice, Inbred BALB C Molecular immunology Monoclonal antibodies Polymerase Chain Reaction Vibrio - immunology
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