Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells
A monoclonal antibody, 17-1a, which reacts with antigen expressed in human colon cancers was radiolabeled in high specific activity with 125I. The combination of the antibody and this radionuclide was observed to elicit specific cellular damage after being internalized into cells of the SW1116 human...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1989-06, Vol.49 (11), p.2952-2958 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | WOO, D. V DERUI LI MATTIS, J. A STEPLEWSKI, Z |
| description | A monoclonal antibody, 17-1a, which reacts with antigen expressed in human colon cancers was radiolabeled in high specific activity with 125I. The combination of the antibody and this radionuclide was observed to elicit specific cellular damage after being internalized into cells of the SW1116 human colon cancer cell line. The degree of internalization was quantitatively measured and found to increase over time to 49% after a 48-h incubation period. During this period, significant chromosome aberrations were observed in the SW1116 cell line due to the Auger electrons of 125I. This damage was not observed using Na125I, a nonimmunoreactive radiolabeled antibody, or cells which did not contain the requisite antigen. The number of chromosomal aberrations increased with increasing radioactive concentration of 125I-17-1a. The nuclear damage resulted in specific cellular cytotoxicity and decreased cell survival of SW1116 cells exposed to various concentrations of 125I-17-1a. |
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V ; DERUI LI ; MATTIS, J. A ; STEPLEWSKI, Z</creator><creatorcontrib>WOO, D. V ; DERUI LI ; MATTIS, J. A ; STEPLEWSKI, Z</creatorcontrib><description>A monoclonal antibody, 17-1a, which reacts with antigen expressed in human colon cancers was radiolabeled in high specific activity with 125I. The combination of the antibody and this radionuclide was observed to elicit specific cellular damage after being internalized into cells of the SW1116 human colon cancer cell line. The degree of internalization was quantitatively measured and found to increase over time to 49% after a 48-h incubation period. During this period, significant chromosome aberrations were observed in the SW1116 cell line due to the Auger electrons of 125I. This damage was not observed using Na125I, a nonimmunoreactive radiolabeled antibody, or cells which did not contain the requisite antigen. The number of chromosomal aberrations increased with increasing radioactive concentration of 125I-17-1a. The nuclear damage resulted in specific cellular cytotoxicity and decreased cell survival of SW1116 cells exposed to various concentrations of 125I-17-1a.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 2720655</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Antibodies, Monoclonal - therapeutic use ; Antigens, Neoplasm - immunology ; Antigens, Neoplasm - metabolism ; Antineoplastic agents ; Biological and medical sciences ; Cell Survival - drug effects ; Chromosome Aberrations ; Colonic Neoplasms - genetics ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - therapy ; DNA Damage ; DNA, Neoplasm - drug effects ; DNA, Neoplasm - metabolism ; General aspects ; Humans ; Iodine Radioisotopes ; Medical sciences ; Pharmacology. Drug treatments ; Tumor Cells, Cultured - metabolism</subject><ispartof>Cancer research (Chicago, Ill.), 1989-06, Vol.49 (11), p.2952-2958</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6795115$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2720655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WOO, D. V</creatorcontrib><creatorcontrib>DERUI LI</creatorcontrib><creatorcontrib>MATTIS, J. A</creatorcontrib><creatorcontrib>STEPLEWSKI, Z</creatorcontrib><title>Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>A monoclonal antibody, 17-1a, which reacts with antigen expressed in human colon cancers was radiolabeled in high specific activity with 125I. The combination of the antibody and this radionuclide was observed to elicit specific cellular damage after being internalized into cells of the SW1116 human colon cancer cell line. The degree of internalization was quantitatively measured and found to increase over time to 49% after a 48-h incubation period. During this period, significant chromosome aberrations were observed in the SW1116 cell line due to the Auger electrons of 125I. This damage was not observed using Na125I, a nonimmunoreactive radiolabeled antibody, or cells which did not contain the requisite antigen. The number of chromosomal aberrations increased with increasing radioactive concentration of 125I-17-1a. The nuclear damage resulted in specific cellular cytotoxicity and decreased cell survival of SW1116 cells exposed to various concentrations of 125I-17-1a.</description><subject>Antibodies, Monoclonal - therapeutic use</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Antigens, Neoplasm - metabolism</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>Chromosome Aberrations</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - therapy</subject><subject>DNA Damage</subject><subject>DNA, Neoplasm - drug effects</subject><subject>DNA, Neoplasm - metabolism</subject><subject>General aspects</subject><subject>Humans</subject><subject>Iodine Radioisotopes</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Tumor Cells, Cultured - metabolism</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9LxDAQxYso67r6EYQcxFshSZMmPcrin4UFD-q5TNOJG2matWnFfnsjFk_D8H5vmPdOsjWThc6VEPI0W1NKdS6F4ufZRYwfaZWMylW24orTUsp15l-wQzO6LyTmMAQfYvDQkRY8vCOBviVmHsMYvp1x40yCJYzLXd5Bk3wt8aEPpgt9skA_uia0M2EqZ0BcTw6Th54Y6A0OxGDXxcvszEIX8WqZm-zt4f51-5Tvnx9327t9fmCslDkKqgUWije0sLZqqxRH6wIq20iohFYWDFBlCmwQG1EhtcyiQF6pknPAYpPd_t09DuFzwjjW3sXfD6DHMMVa6UoXJdUJvF7AqfHY1sfBeRjmeukn6TeLDtFAZ4cUxsV_rFSVZKnwHxZRb4A</recordid><startdate>19890601</startdate><enddate>19890601</enddate><creator>WOO, D. V</creator><creator>DERUI LI</creator><creator>MATTIS, J. A</creator><creator>STEPLEWSKI, Z</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19890601</creationdate><title>Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells</title><author>WOO, D. V ; DERUI LI ; MATTIS, J. A ; STEPLEWSKI, Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h1165-e4084e372b03ff9d9744883a9fb5a9487faca07c3ebeeb49e0f1fe4e297622ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Antibodies, Monoclonal - therapeutic use</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Antigens, Neoplasm - metabolism</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Cell Survival - drug effects</topic><topic>Chromosome Aberrations</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - therapy</topic><topic>DNA Damage</topic><topic>DNA, Neoplasm - drug effects</topic><topic>DNA, Neoplasm - metabolism</topic><topic>General aspects</topic><topic>Humans</topic><topic>Iodine Radioisotopes</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Tumor Cells, Cultured - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WOO, D. V</creatorcontrib><creatorcontrib>DERUI LI</creatorcontrib><creatorcontrib>MATTIS, J. A</creatorcontrib><creatorcontrib>STEPLEWSKI, Z</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WOO, D. V</au><au>DERUI LI</au><au>MATTIS, J. A</au><au>STEPLEWSKI, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1989-06-01</date><risdate>1989</risdate><volume>49</volume><issue>11</issue><spage>2952</spage><epage>2958</epage><pages>2952-2958</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>A monoclonal antibody, 17-1a, which reacts with antigen expressed in human colon cancers was radiolabeled in high specific activity with 125I. The combination of the antibody and this radionuclide was observed to elicit specific cellular damage after being internalized into cells of the SW1116 human colon cancer cell line. The degree of internalization was quantitatively measured and found to increase over time to 49% after a 48-h incubation period. During this period, significant chromosome aberrations were observed in the SW1116 cell line due to the Auger electrons of 125I. This damage was not observed using Na125I, a nonimmunoreactive radiolabeled antibody, or cells which did not contain the requisite antigen. The number of chromosomal aberrations increased with increasing radioactive concentration of 125I-17-1a. The nuclear damage resulted in specific cellular cytotoxicity and decreased cell survival of SW1116 cells exposed to various concentrations of 125I-17-1a.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>2720655</pmid><tpages>7</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1989-06, Vol.49 (11), p.2952-2958 |
| issn | 0008-5472 1538-7445 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; American Association for Cancer Research |
| subjects | Antibodies, Monoclonal - therapeutic use Antigens, Neoplasm - immunology Antigens, Neoplasm - metabolism Antineoplastic agents Biological and medical sciences Cell Survival - drug effects Chromosome Aberrations Colonic Neoplasms - genetics Colonic Neoplasms - metabolism Colonic Neoplasms - therapy DNA Damage DNA, Neoplasm - drug effects DNA, Neoplasm - metabolism General aspects Humans Iodine Radioisotopes Medical sciences Pharmacology. Drug treatments Tumor Cells, Cultured - metabolism |
| title | Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells |
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