Influence of methisoprinol (lsoprinosine) on hiv-infected human lymphocytes: In vitro immunological, virological, and ultrastructural studies
Methisoprinol (isoprinosine) is a synthetic compound with reported antiviral and immunomodulating properties. Results of the present study showed that methisoprinol at concentrations ≥ 200 μg/ml reduces the p24 and gp120 viral antigen expression on the surface of human immunodeficiency virus (HIV)‐i...
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| Veröffentlicht in: | Journal of clinical laboratory analysis 1989, Vol.3 (1), p.26-33 |
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| container_title | Journal of clinical laboratory analysis |
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| creator | De Simone, Claudio DeMarco, Federico Arancia, Giuseppe Tzantzoglou, Sonia Paradisi, Silvia Sorice, Franco |
| description | Methisoprinol (isoprinosine) is a synthetic compound with reported antiviral and immunomodulating properties. Results of the present study showed that methisoprinol at concentrations ≥ 200 μg/ml reduces the p24 and gp120 viral antigen expression on the surface of human immunodeficiency virus (HIV)‐infected lymphocytes and the reverse transcriptase levels. In addition, cell viability, the number of the CD4 + cells, and the CD4 + /CD8+ cell ratio are higher in methisoprinol‐pretreated cell suspensions than in untreated HIV‐infected cell cultures. A quantitative freeze‐fracture study on the density of the intramembranous particles (IMP) present on both fracture faces of the plasma membrane of lymphocytes has shown that pretreatment with methisoprinol induces a different molecular organization resulting in a nearly three times increase of IMP density. |
| doi_str_mv | 10.1002/jcla.1860030107 |
| format | Article |
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Results of the present study showed that methisoprinol at concentrations ≥ 200 μg/ml reduces the p24 and gp120 viral antigen expression on the surface of human immunodeficiency virus (HIV)‐infected lymphocytes and the reverse transcriptase levels. In addition, cell viability, the number of the CD4 + cells, and the CD4 + /CD8+ cell ratio are higher in methisoprinol‐pretreated cell suspensions than in untreated HIV‐infected cell cultures. A quantitative freeze‐fracture study on the density of the intramembranous particles (IMP) present on both fracture faces of the plasma membrane of lymphocytes has shown that pretreatment with methisoprinol induces a different molecular organization resulting in a nearly three times increase of IMP density.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.1860030107</identifier><identifier>PMID: 2469781</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Acquired Immunodeficiency Syndrome - blood ; Acquired Immunodeficiency Syndrome - drug therapy ; Acquired Immunodeficiency Syndrome - microbiology ; Adult ; AIDS ; AIDS/HIV ; Cell Membrane - drug effects ; Cell Membrane - ultrastructure ; Cytopathogenic Effect, Viral - drug effects ; HIV ; HIV - drug effects ; HIV - isolation & purification ; human immunodeficiency virus ; Humans ; In Vitro Techniques ; Inosine - analogs & derivatives ; Inosine Pranobex - pharmacology ; isoprinosine ; Lymphocytes - drug effects ; Lymphocytes - microbiology ; Lymphocytes - ultrastructure ; methisoprinol ; Microscopy, Electron</subject><ispartof>Journal of clinical laboratory analysis, 1989, Vol.3 (1), p.26-33</ispartof><rights>Copyright © 1989 Wiley Periodicals, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3287-bc8f9989b02ebf9ac021e716bbcde4d44d5edb0cb94be405fb13c65105d293503</citedby><cites>FETCH-LOGICAL-c3287-bc8f9989b02ebf9ac021e716bbcde4d44d5edb0cb94be405fb13c65105d293503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcla.1860030107$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcla.1860030107$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2469781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Simone, Claudio</creatorcontrib><creatorcontrib>DeMarco, Federico</creatorcontrib><creatorcontrib>Arancia, Giuseppe</creatorcontrib><creatorcontrib>Tzantzoglou, Sonia</creatorcontrib><creatorcontrib>Paradisi, Silvia</creatorcontrib><creatorcontrib>Sorice, Franco</creatorcontrib><title>Influence of methisoprinol (lsoprinosine) on hiv-infected human lymphocytes: In vitro immunological, virological, and ultrastructural studies</title><title>Journal of clinical laboratory analysis</title><addtitle>J. Clin. Lab. Anal</addtitle><description>Methisoprinol (isoprinosine) is a synthetic compound with reported antiviral and immunomodulating properties. Results of the present study showed that methisoprinol at concentrations ≥ 200 μg/ml reduces the p24 and gp120 viral antigen expression on the surface of human immunodeficiency virus (HIV)‐infected lymphocytes and the reverse transcriptase levels. In addition, cell viability, the number of the CD4 + cells, and the CD4 + /CD8+ cell ratio are higher in methisoprinol‐pretreated cell suspensions than in untreated HIV‐infected cell cultures. A quantitative freeze‐fracture study on the density of the intramembranous particles (IMP) present on both fracture faces of the plasma membrane of lymphocytes has shown that pretreatment with methisoprinol induces a different molecular organization resulting in a nearly three times increase of IMP density.</description><subject>Acquired Immunodeficiency Syndrome - blood</subject><subject>Acquired Immunodeficiency Syndrome - drug therapy</subject><subject>Acquired Immunodeficiency Syndrome - microbiology</subject><subject>Adult</subject><subject>AIDS</subject><subject>AIDS/HIV</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cytopathogenic Effect, Viral - drug effects</subject><subject>HIV</subject><subject>HIV - drug effects</subject><subject>HIV - isolation & purification</subject><subject>human immunodeficiency virus</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Inosine - analogs & derivatives</subject><subject>Inosine Pranobex - pharmacology</subject><subject>isoprinosine</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - microbiology</subject><subject>Lymphocytes - ultrastructure</subject><subject>methisoprinol</subject><subject>Microscopy, Electron</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhS0EKqGwZoXkFSoS017P06arEkoIjUBIPJaWx3OHuHjsYHsK-RH9z0yVqBWrrmzfc84nXR9CnjM4ZgD5yaW26pjxGqAABs0DMmMgeJbzvHpIZsB5k3FgxWPyJMZLAOCC1QfkIC9r0XA2I9dL19sRnUbqezpgWpvoN8E4b-mR3V-jcfiKekfX5iozrkedsKPrcVCO2u2wWXu9TRjf0KWjVyYFT80wjBPC_zRa2dfTMNw9lOvoaFNQMYVRpzEoS2MaO4PxKXnUKxvx2f48JN_en3-df8hWnxfL-dkq00U-bdRq3gvBRQs5tr1QGnKGDavbVndYdmXZVdi1oFtRtlhC1bes0HXFoOpyUVRQHJKXO-4m-N8jxiQHEzVaqxz6McqGC85Ek99rZBWr67poJuPJzqiDjzFgL6efG1TYSgbypil505S8a2pKvNijx3bA7ta_r2bST3f6H2Nxex9Ofpyvzv6jZ7u0iQn_3qZV-CXrpmgq-ePTQn75Dm8vFhe1fFf8A4_As6w</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>De Simone, Claudio</creator><creator>DeMarco, Federico</creator><creator>Arancia, Giuseppe</creator><creator>Tzantzoglou, Sonia</creator><creator>Paradisi, Silvia</creator><creator>Sorice, Franco</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Influence of methisoprinol (lsoprinosine) on hiv-infected human lymphocytes: In vitro immunological, virological, and ultrastructural studies</title><author>De Simone, Claudio ; DeMarco, Federico ; Arancia, Giuseppe ; Tzantzoglou, Sonia ; Paradisi, Silvia ; Sorice, Franco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3287-bc8f9989b02ebf9ac021e716bbcde4d44d5edb0cb94be405fb13c65105d293503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Acquired Immunodeficiency Syndrome - blood</topic><topic>Acquired Immunodeficiency Syndrome - drug therapy</topic><topic>Acquired Immunodeficiency Syndrome - microbiology</topic><topic>Adult</topic><topic>AIDS</topic><topic>AIDS/HIV</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cytopathogenic Effect, Viral - drug effects</topic><topic>HIV</topic><topic>HIV - drug effects</topic><topic>HIV - isolation & purification</topic><topic>human immunodeficiency virus</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Inosine - analogs & derivatives</topic><topic>Inosine Pranobex - pharmacology</topic><topic>isoprinosine</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - microbiology</topic><topic>Lymphocytes - ultrastructure</topic><topic>methisoprinol</topic><topic>Microscopy, Electron</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Simone, Claudio</creatorcontrib><creatorcontrib>DeMarco, Federico</creatorcontrib><creatorcontrib>Arancia, Giuseppe</creatorcontrib><creatorcontrib>Tzantzoglou, Sonia</creatorcontrib><creatorcontrib>Paradisi, Silvia</creatorcontrib><creatorcontrib>Sorice, Franco</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Simone, Claudio</au><au>DeMarco, Federico</au><au>Arancia, Giuseppe</au><au>Tzantzoglou, Sonia</au><au>Paradisi, Silvia</au><au>Sorice, Franco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of methisoprinol (lsoprinosine) on hiv-infected human lymphocytes: In vitro immunological, virological, and ultrastructural studies</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J. Clin. Lab. Anal</addtitle><date>1989</date><risdate>1989</risdate><volume>3</volume><issue>1</issue><spage>26</spage><epage>33</epage><pages>26-33</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>Methisoprinol (isoprinosine) is a synthetic compound with reported antiviral and immunomodulating properties. Results of the present study showed that methisoprinol at concentrations ≥ 200 μg/ml reduces the p24 and gp120 viral antigen expression on the surface of human immunodeficiency virus (HIV)‐infected lymphocytes and the reverse transcriptase levels. In addition, cell viability, the number of the CD4 + cells, and the CD4 + /CD8+ cell ratio are higher in methisoprinol‐pretreated cell suspensions than in untreated HIV‐infected cell cultures. 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| ispartof | Journal of clinical laboratory analysis, 1989, Vol.3 (1), p.26-33 |
| issn | 0887-8013 1098-2825 |
| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Freely accessible e-journals |
| subjects | Acquired Immunodeficiency Syndrome - blood Acquired Immunodeficiency Syndrome - drug therapy Acquired Immunodeficiency Syndrome - microbiology Adult AIDS AIDS/HIV Cell Membrane - drug effects Cell Membrane - ultrastructure Cytopathogenic Effect, Viral - drug effects HIV HIV - drug effects HIV - isolation & purification human immunodeficiency virus Humans In Vitro Techniques Inosine - analogs & derivatives Inosine Pranobex - pharmacology isoprinosine Lymphocytes - drug effects Lymphocytes - microbiology Lymphocytes - ultrastructure methisoprinol Microscopy, Electron |
| title | Influence of methisoprinol (lsoprinosine) on hiv-infected human lymphocytes: In vitro immunological, virological, and ultrastructural studies |
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