Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy, and flow cytometry

p53 immunostaining of histological sections shows inter‐ and intratumor variability in distribution and staining intensity which are usually scored semiquantitatively. In order to investigate the variation in p53 expression more accurately and its possible relation to other cellular parameters (e.g....

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Veröffentlicht in:	Cytometry (New York, N.Y.) N.Y.), 1997-05, Vol.28 (1), p.11-24
Hauptverfasser:	Bonsing, Bert A., Corver, Willem E., Gorsira, Michael C. B., van Vliet, Margreethe, Oud, Peter S., Cornelisse, Cees J., Fleuren, Gert Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal Antibody Specificity Blotting, Western breast carcinoma Breast Neoplasms - metabolism Breast Neoplasms - pathology confocal laser scanning microscopy DNA Mutational Analysis Female Flow Cytometry Fluorescent Antibody Technique, Indirect Genes, p53 - genetics Humans Immunohistochemistry Lymphoma - metabolism Lymphoma - pathology Microscopy, Confocal monoclonal antibodies Mutation Neoplasms - metabolism Neoplasms - pathology Osteosarcoma - metabolism Osteosarcoma - pathology Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology p53 Tumor Cells, Cultured tumor heterogeneity Tumor Suppressor Protein p53 - analysis Tumor Suppressor Protein p53 - immunology Western blotting
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