Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived from Normals and Patients with Cystic Fibrosis

Human sweat duct cells from the coiled reabsorptive segment have been cultured successfully, free from fibroblasts, in a low serum, hormone-supplemented medium, Ham's F12. The cultured cells exhibited a typical epithelial cobblestone pattern, and microvilli-covered luminal cells were seen joine...

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Veröffentlicht in:	In Vitro Cellular & Developmental Biology 1989-04, Vol.25 (4), p.342-352
1. Verfasser:	Pedersen, Peter Steen
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Amiloride - pharmacology Animal cells Biological and medical sciences Cell culture techniques Cell cultures. Hybridization. Fusion Cell growth Cell membranes Cells, Cultured Child Child, Preschool Culture Media Cultured cells Cystic fibrosis Cystic Fibrosis - metabolism Cystic Fibrosis - pathology Electric Conductivity Epithelial Cells Epithelium Fundamental and applied biological sciences. Psychology Humans Membrane Potentials Molecular and cellular biology P branes Potassium Channels - metabolism Sodium Sodium Channels - metabolism Sweat Sweat Glands - cytology Sweat Glands - metabolism Sweat Glands - pathology Temperature
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