Membrane Fluidity and Oxygen Diffusion in Cholesterol-Enriched Erythrocyte Membrane
This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol–protein ratio (C/Pt). The membrane fluidity...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1997-05, Vol.341 (1), p.34-39 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Dumas, Dominique Muller, Sylvaine Gouin, Frédérique Baros, Francis Viriot, Marie-Laure Stoltz, Jean-François |
| description | This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol–protein ratio (C/Pt). The membrane fluidity, as assessed by a fluorescence polarization method with diphenylhexatriene and 1-(4-trimethylamino)-6-phenylhexa-1,3,5-triene, decreased as the C/Pt ratio increased. Time-resolved fluorescence spectroscopy of pyrene dodecanoic acid (PDA) under an increasing C/Pt ratio in the erythrocyte membrane revealed enhanced oxygen diffusion in the middle of the membrane bilayer (in which PDA was incorporated), which was not the case with pyrene butyric acid (PBA) incorporated in the internal part of the membrane surface. It has generally been accepted that increased membrane fluidity reduces the physical barrier to oxygen permeation. Such conflicting observations on oxygen permeation in the rigidified erythrocyte membrane could be due to variations in oxygen solubility (preferential partitioning) in different polarity microdomains (cholesterol and phospholipid partitions). |
| doi_str_mv | 10.1006/abbi.1997.9936 |
| format | Article |
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Cholesterol concentration in the membrane was expressed as the cholesterol–protein ratio (C/Pt). The membrane fluidity, as assessed by a fluorescence polarization method with diphenylhexatriene and 1-(4-trimethylamino)-6-phenylhexa-1,3,5-triene, decreased as the C/Pt ratio increased. Time-resolved fluorescence spectroscopy of pyrene dodecanoic acid (PDA) under an increasing C/Pt ratio in the erythrocyte membrane revealed enhanced oxygen diffusion in the middle of the membrane bilayer (in which PDA was incorporated), which was not the case with pyrene butyric acid (PBA) incorporated in the internal part of the membrane surface. It has generally been accepted that increased membrane fluidity reduces the physical barrier to oxygen permeation. Such conflicting observations on oxygen permeation in the rigidified erythrocyte membrane could be due to variations in oxygen solubility (preferential partitioning) in different polarity microdomains (cholesterol and phospholipid partitions).</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1006/abbi.1997.9936</identifier><identifier>PMID: 9143350</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>cholesterol ; Cholesterol - analysis ; Cholesterol - physiology ; Cholesterol Esters - metabolism ; Cholesterol Esters - pharmacology ; Diffusion ; erythrocyte membrane ; Erythrocyte Membrane - chemistry ; Erythrocyte Membrane - metabolism ; Fluorescence Polarization ; fluorescence quenching ; Humans ; membrane fluidity ; Membrane Fluidity - drug effects ; Membrane Fluidity - physiology ; Molecular Probes - metabolism ; Molecular Structure ; Oxygen - metabolism ; oxygen diffusion ; Pyrenes - metabolism ; Temperature</subject><ispartof>Archives of biochemistry and biophysics, 1997-05, Vol.341 (1), p.34-39</ispartof><rights>1997 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-39841bc4e85be54129fd964915de2f24f71e2b70b78fba57e57e7e6dda33335e3</citedby><cites>FETCH-LOGICAL-c339t-39841bc4e85be54129fd964915de2f24f71e2b70b78fba57e57e7e6dda33335e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003986197999367$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9143350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dumas, Dominique</creatorcontrib><creatorcontrib>Muller, Sylvaine</creatorcontrib><creatorcontrib>Gouin, Frédérique</creatorcontrib><creatorcontrib>Baros, Francis</creatorcontrib><creatorcontrib>Viriot, Marie-Laure</creatorcontrib><creatorcontrib>Stoltz, Jean-François</creatorcontrib><title>Membrane Fluidity and Oxygen Diffusion in Cholesterol-Enriched Erythrocyte Membrane</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol–protein ratio (C/Pt). The membrane fluidity, as assessed by a fluorescence polarization method with diphenylhexatriene and 1-(4-trimethylamino)-6-phenylhexa-1,3,5-triene, decreased as the C/Pt ratio increased. Time-resolved fluorescence spectroscopy of pyrene dodecanoic acid (PDA) under an increasing C/Pt ratio in the erythrocyte membrane revealed enhanced oxygen diffusion in the middle of the membrane bilayer (in which PDA was incorporated), which was not the case with pyrene butyric acid (PBA) incorporated in the internal part of the membrane surface. It has generally been accepted that increased membrane fluidity reduces the physical barrier to oxygen permeation. Such conflicting observations on oxygen permeation in the rigidified erythrocyte membrane could be due to variations in oxygen solubility (preferential partitioning) in different polarity microdomains (cholesterol and phospholipid partitions).</description><subject>cholesterol</subject><subject>Cholesterol - analysis</subject><subject>Cholesterol - physiology</subject><subject>Cholesterol Esters - metabolism</subject><subject>Cholesterol Esters - pharmacology</subject><subject>Diffusion</subject><subject>erythrocyte membrane</subject><subject>Erythrocyte Membrane - chemistry</subject><subject>Erythrocyte Membrane - metabolism</subject><subject>Fluorescence Polarization</subject><subject>fluorescence quenching</subject><subject>Humans</subject><subject>membrane fluidity</subject><subject>Membrane Fluidity - drug effects</subject><subject>Membrane Fluidity - physiology</subject><subject>Molecular Probes - metabolism</subject><subject>Molecular Structure</subject><subject>Oxygen - metabolism</subject><subject>oxygen diffusion</subject><subject>Pyrenes - metabolism</subject><subject>Temperature</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL1PwzAQxS0EKqWwsiFlYkuwYyeOR1RaQAJ1AGYrts_UKB_FThD570nUwsbppBvu3dO7H0KXBCcE4_ymVMolRAieCEHzIzQnWOQxpgU7RnOMMY1FkZNTdBbCB8aEsDydoZkgjNIMz9HLM9TKlw1E66p3xnVDVDYm2nwP79BEd87aPri2iVwTLbdtBaED31bxqvFOb8FEKz90W9_qoYPo1-ocndiyCnBxmAv0tl69Lh_ip8394_L2KdaUii6momBEaQZFpiBjJBXWiJwJkhlIbcosJ5AqjhUvrCozDmNzyI0p6VgZ0AW63vvufPvZj9Fk7YKGqhoztH2QvBA8FVk-CpO9UPs2BA9W7ryrSz9IguVEUU4U5URRThTHg6uDc69qMH_yA7ZxX-z3ML735cDLoB00GozzoDtpWvef9Q_WHoGR</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>Dumas, Dominique</creator><creator>Muller, Sylvaine</creator><creator>Gouin, Frédérique</creator><creator>Baros, Francis</creator><creator>Viriot, Marie-Laure</creator><creator>Stoltz, Jean-François</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970501</creationdate><title>Membrane Fluidity and Oxygen Diffusion in Cholesterol-Enriched Erythrocyte Membrane</title><author>Dumas, Dominique ; Muller, Sylvaine ; Gouin, Frédérique ; Baros, Francis ; Viriot, Marie-Laure ; Stoltz, Jean-François</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-39841bc4e85be54129fd964915de2f24f71e2b70b78fba57e57e7e6dda33335e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>cholesterol</topic><topic>Cholesterol - analysis</topic><topic>Cholesterol - physiology</topic><topic>Cholesterol Esters - metabolism</topic><topic>Cholesterol Esters - pharmacology</topic><topic>Diffusion</topic><topic>erythrocyte membrane</topic><topic>Erythrocyte Membrane - chemistry</topic><topic>Erythrocyte Membrane - metabolism</topic><topic>Fluorescence Polarization</topic><topic>fluorescence quenching</topic><topic>Humans</topic><topic>membrane fluidity</topic><topic>Membrane Fluidity - drug effects</topic><topic>Membrane Fluidity - physiology</topic><topic>Molecular Probes - metabolism</topic><topic>Molecular Structure</topic><topic>Oxygen - metabolism</topic><topic>oxygen diffusion</topic><topic>Pyrenes - metabolism</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dumas, Dominique</creatorcontrib><creatorcontrib>Muller, Sylvaine</creatorcontrib><creatorcontrib>Gouin, Frédérique</creatorcontrib><creatorcontrib>Baros, Francis</creatorcontrib><creatorcontrib>Viriot, Marie-Laure</creatorcontrib><creatorcontrib>Stoltz, Jean-François</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dumas, Dominique</au><au>Muller, Sylvaine</au><au>Gouin, Frédérique</au><au>Baros, Francis</au><au>Viriot, Marie-Laure</au><au>Stoltz, Jean-François</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Membrane Fluidity and Oxygen Diffusion in Cholesterol-Enriched Erythrocyte Membrane</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>341</volume><issue>1</issue><spage>34</spage><epage>39</epage><pages>34-39</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol–protein ratio (C/Pt). The membrane fluidity, as assessed by a fluorescence polarization method with diphenylhexatriene and 1-(4-trimethylamino)-6-phenylhexa-1,3,5-triene, decreased as the C/Pt ratio increased. Time-resolved fluorescence spectroscopy of pyrene dodecanoic acid (PDA) under an increasing C/Pt ratio in the erythrocyte membrane revealed enhanced oxygen diffusion in the middle of the membrane bilayer (in which PDA was incorporated), which was not the case with pyrene butyric acid (PBA) incorporated in the internal part of the membrane surface. It has generally been accepted that increased membrane fluidity reduces the physical barrier to oxygen permeation. Such conflicting observations on oxygen permeation in the rigidified erythrocyte membrane could be due to variations in oxygen solubility (preferential partitioning) in different polarity microdomains (cholesterol and phospholipid partitions).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9143350</pmid><doi>10.1006/abbi.1997.9936</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | cholesterol Cholesterol - analysis Cholesterol - physiology Cholesterol Esters - metabolism Cholesterol Esters - pharmacology Diffusion erythrocyte membrane Erythrocyte Membrane - chemistry Erythrocyte Membrane - metabolism Fluorescence Polarization fluorescence quenching Humans membrane fluidity Membrane Fluidity - drug effects Membrane Fluidity - physiology Molecular Probes - metabolism Molecular Structure Oxygen - metabolism oxygen diffusion Pyrenes - metabolism Temperature |
| title | Membrane Fluidity and Oxygen Diffusion in Cholesterol-Enriched Erythrocyte Membrane |
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