Coagulase and protein A polymorphisms do not contribute to persistence of nasal colonisation by Staphylococcus aureus
Department Of Bacteriology, University Hospital Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands * Statens Seruminstitut, Staphylococcus Laboratory, Artillerivej 5, DK-2300 Copenhagen S. Denmark 2 Corresponding author: Dr A. van Belkum. Received August 12, 1996 Accepted August 23,...
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| Veröffentlicht in: | Journal of medical microbiology 1997-03, Vol.46 (3), p.222-232 |
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| creator | Van Belkum, A Eriksen, N. H. Riewarts Sijmons, Marly Van Leeuwen, W Van Den Bergh, Marjolein Kluytmans, Jan Espersen, F Verbrugh, H |
| description | Department Of Bacteriology, University Hospital Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
* Statens Seruminstitut, Staphylococcus Laboratory, Artillerivej 5, DK-2300 Copenhagen S. Denmark
2 Corresponding author: Dr A. van Belkum.
Received August 12, 1996
Accepted August 23, 1996
The nasal carriage rate of Staphylococcus aureus was examined in a longitudinal study of 31 healthy Danish volunteers. Each person was classified as persistent (>8 positive cultures from 10 examinations), an intermittent carrier (50–80% positive cultures) or an ocassional carrier (positive cultures on 10–40% of ocassions only). One hundred and twenty strains from these persons were subjected to phage typing and random amplification of polymorphic DNA (RAPD) analysis. Phage and RAPD typing were in close agreement. RAPD confirmed the spread of a particular S. aureus clone (phage type 95) throughout Denmark. However, no common genotype or phenotype characteristics of S. aureus that could separate persistent from intermittent or incidental colonisers were identified. The immunoglobulin binding protein A and the prothrombin binding coagulase protein are both putative S. aureus virulence or defence factors. Analysis of polymorphisms in the variable repeat regions in the genes for these proteins showed no correlation between the number of repeat units and, consequently, the protein structure with the ability of strains to persist in the human nasal mucosa. The amount of protein A, detectable by its IgG binding activity, appeared not to be correlated to persistence of carriage. Thus protein A and coagulase -gene polymorphisms do not seem to play a significant role in the propensity of S. aureus to colonise human nasal epithelium. Furthermore, based on the genetic heterogeneity encountered among the S. aureus strains it is suggested that within the current study population, no single clonal lineage of S. aureus has increased capability to colonise the human nasal epithelium. |
| doi_str_mv | 10.1099/00222615-46-3-222 |
| format | Article |
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* Statens Seruminstitut, Staphylococcus Laboratory, Artillerivej 5, DK-2300 Copenhagen S. Denmark
2 Corresponding author: Dr A. van Belkum.
Received August 12, 1996
Accepted August 23, 1996
The nasal carriage rate of Staphylococcus aureus was examined in a longitudinal study of 31 healthy Danish volunteers. Each person was classified as persistent (>8 positive cultures from 10 examinations), an intermittent carrier (50–80% positive cultures) or an ocassional carrier (positive cultures on 10–40% of ocassions only). One hundred and twenty strains from these persons were subjected to phage typing and random amplification of polymorphic DNA (RAPD) analysis. Phage and RAPD typing were in close agreement. RAPD confirmed the spread of a particular S. aureus clone (phage type 95) throughout Denmark. However, no common genotype or phenotype characteristics of S. aureus that could separate persistent from intermittent or incidental colonisers were identified. The immunoglobulin binding protein A and the prothrombin binding coagulase protein are both putative S. aureus virulence or defence factors. Analysis of polymorphisms in the variable repeat regions in the genes for these proteins showed no correlation between the number of repeat units and, consequently, the protein structure with the ability of strains to persist in the human nasal mucosa. The amount of protein A, detectable by its IgG binding activity, appeared not to be correlated to persistence of carriage. Thus protein A and coagulase -gene polymorphisms do not seem to play a significant role in the propensity of S. aureus to colonise human nasal epithelium. Furthermore, based on the genetic heterogeneity encountered among the S. aureus strains it is suggested that within the current study population, no single clonal lineage of S. aureus has increased capability to colonise the human nasal epithelium.</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/00222615-46-3-222</identifier><identifier>PMID: 9126823</identifier><identifier>CODEN: JMMIAV</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Adult ; Aged ; Bacteriology ; Bacteriophage Typing ; Biological and medical sciences ; Carrier State - microbiology ; Coagulase - genetics ; DNA, Bacterial - analysis ; Female ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genotype ; Humans ; Longitudinal Studies ; Male ; Microbiology ; Middle Aged ; Nasal Mucosa - microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Phenotype ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Random Amplified Polymorphic DNA Technique ; Staphylococcal Infections - microbiology ; Staphylococcal Protein A - genetics ; Staphylococcus aureus - classification ; Staphylococcus aureus - genetics ; Staphylococcus aureus - pathogenicity ; Virulence</subject><ispartof>Journal of medical microbiology, 1997-03, Vol.46 (3), p.222-232</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-9aab51c09e53ecd6603d6edd7e89b700627caa6dee4f23d49a17d72fb3e25cee3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3733,3734,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2696471$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9126823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Belkum, A</creatorcontrib><creatorcontrib>Eriksen, N. H. Riewarts</creatorcontrib><creatorcontrib>Sijmons, Marly</creatorcontrib><creatorcontrib>Van Leeuwen, W</creatorcontrib><creatorcontrib>Van Den Bergh, Marjolein</creatorcontrib><creatorcontrib>Kluytmans, Jan</creatorcontrib><creatorcontrib>Espersen, F</creatorcontrib><creatorcontrib>Verbrugh, H</creatorcontrib><title>Coagulase and protein A polymorphisms do not contribute to persistence of nasal colonisation by Staphylococcus aureus</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>Department Of Bacteriology, University Hospital Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
* Statens Seruminstitut, Staphylococcus Laboratory, Artillerivej 5, DK-2300 Copenhagen S. Denmark
2 Corresponding author: Dr A. van Belkum.
Received August 12, 1996
Accepted August 23, 1996
The nasal carriage rate of Staphylococcus aureus was examined in a longitudinal study of 31 healthy Danish volunteers. Each person was classified as persistent (>8 positive cultures from 10 examinations), an intermittent carrier (50–80% positive cultures) or an ocassional carrier (positive cultures on 10–40% of ocassions only). One hundred and twenty strains from these persons were subjected to phage typing and random amplification of polymorphic DNA (RAPD) analysis. Phage and RAPD typing were in close agreement. RAPD confirmed the spread of a particular S. aureus clone (phage type 95) throughout Denmark. However, no common genotype or phenotype characteristics of S. aureus that could separate persistent from intermittent or incidental colonisers were identified. The immunoglobulin binding protein A and the prothrombin binding coagulase protein are both putative S. aureus virulence or defence factors. Analysis of polymorphisms in the variable repeat regions in the genes for these proteins showed no correlation between the number of repeat units and, consequently, the protein structure with the ability of strains to persist in the human nasal mucosa. The amount of protein A, detectable by its IgG binding activity, appeared not to be correlated to persistence of carriage. Thus protein A and coagulase -gene polymorphisms do not seem to play a significant role in the propensity of S. aureus to colonise human nasal epithelium. Furthermore, based on the genetic heterogeneity encountered among the S. aureus strains it is suggested that within the current study population, no single clonal lineage of S. aureus has increased capability to colonise the human nasal epithelium.</description><subject>Adult</subject><subject>Aged</subject><subject>Bacteriology</subject><subject>Bacteriophage Typing</subject><subject>Biological and medical sciences</subject><subject>Carrier State - microbiology</subject><subject>Coagulase - genetics</subject><subject>DNA, Bacterial - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Humans</subject><subject>Longitudinal Studies</subject><subject>Male</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Nasal Mucosa - microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Protein A - genetics</subject><subject>Staphylococcus aureus - classification</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Virulence</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoY8_oD3AhZCG6Ks2rks5yaNQRBlyo65BKbnVnqErKPJD-91bTbW9d3QPnuy8OQm8o-UiJ1p8IYYxJ2ndCdrxb9TO0oULxrpdCPEebk9-dgJfotpQnQqjiXN-gG02Z3DK-QW2X7L5NtgC20eMlpwoh4nu8pOk4p7wcQpkL9gnHVLFLseYwtAq4JrxALqFUiA5wGnG0xU4rMqUYiq0hRTwc8Y9ql8NxSi451wq2LUMrr9CL0U4FXl_qHfr15fPP3UP3-P3rt939Y-cEp7XT1g49dURDz8F5KQn3ErxXsNWDIkQy5ayVHkCMjHuhLVVesXHgwHoHwO_Q-_Pc9a_fDUo1cygOpslGSK0YtdWK9YL_F6S9Zpz0agXpGXQ5lZJhNEsOs81HQ4k5ZWL-ZWKENNyseu15exnehhn8teMSwuq_u_i2ODuN2UYXyhVjUkuh6Ip9OGOHsD_8CRnMHuIc1kOGkMzTPF8X_gULE6SO</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>Van Belkum, A</creator><creator>Eriksen, N. H. Riewarts</creator><creator>Sijmons, Marly</creator><creator>Van Leeuwen, W</creator><creator>Van Den Bergh, Marjolein</creator><creator>Kluytmans, Jan</creator><creator>Espersen, F</creator><creator>Verbrugh, H</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19970301</creationdate><title>Coagulase and protein A polymorphisms do not contribute to persistence of nasal colonisation by Staphylococcus aureus</title><author>Van Belkum, A ; Eriksen, N. H. Riewarts ; Sijmons, Marly ; Van Leeuwen, W ; Van Den Bergh, Marjolein ; Kluytmans, Jan ; Espersen, F ; Verbrugh, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-9aab51c09e53ecd6603d6edd7e89b700627caa6dee4f23d49a17d72fb3e25cee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Bacteriology</topic><topic>Bacteriophage Typing</topic><topic>Biological and medical sciences</topic><topic>Carrier State - microbiology</topic><topic>Coagulase - genetics</topic><topic>DNA, Bacterial - analysis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Humans</topic><topic>Longitudinal Studies</topic><topic>Male</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Nasal Mucosa - microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Protein A - genetics</topic><topic>Staphylococcus aureus - classification</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - pathogenicity</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Belkum, A</creatorcontrib><creatorcontrib>Eriksen, N. H. Riewarts</creatorcontrib><creatorcontrib>Sijmons, Marly</creatorcontrib><creatorcontrib>Van Leeuwen, W</creatorcontrib><creatorcontrib>Van Den Bergh, Marjolein</creatorcontrib><creatorcontrib>Kluytmans, Jan</creatorcontrib><creatorcontrib>Espersen, F</creatorcontrib><creatorcontrib>Verbrugh, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Belkum, A</au><au>Eriksen, N. H. Riewarts</au><au>Sijmons, Marly</au><au>Van Leeuwen, W</au><au>Van Den Bergh, Marjolein</au><au>Kluytmans, Jan</au><au>Espersen, F</au><au>Verbrugh, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coagulase and protein A polymorphisms do not contribute to persistence of nasal colonisation by Staphylococcus aureus</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>1997-03-01</date><risdate>1997</risdate><volume>46</volume><issue>3</issue><spage>222</spage><epage>232</epage><pages>222-232</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>Department Of Bacteriology, University Hospital Dijkzigt, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
* Statens Seruminstitut, Staphylococcus Laboratory, Artillerivej 5, DK-2300 Copenhagen S. Denmark
2 Corresponding author: Dr A. van Belkum.
Received August 12, 1996
Accepted August 23, 1996
The nasal carriage rate of Staphylococcus aureus was examined in a longitudinal study of 31 healthy Danish volunteers. Each person was classified as persistent (>8 positive cultures from 10 examinations), an intermittent carrier (50–80% positive cultures) or an ocassional carrier (positive cultures on 10–40% of ocassions only). One hundred and twenty strains from these persons were subjected to phage typing and random amplification of polymorphic DNA (RAPD) analysis. Phage and RAPD typing were in close agreement. RAPD confirmed the spread of a particular S. aureus clone (phage type 95) throughout Denmark. However, no common genotype or phenotype characteristics of S. aureus that could separate persistent from intermittent or incidental colonisers were identified. The immunoglobulin binding protein A and the prothrombin binding coagulase protein are both putative S. aureus virulence or defence factors. Analysis of polymorphisms in the variable repeat regions in the genes for these proteins showed no correlation between the number of repeat units and, consequently, the protein structure with the ability of strains to persist in the human nasal mucosa. The amount of protein A, detectable by its IgG binding activity, appeared not to be correlated to persistence of carriage. Thus protein A and coagulase -gene polymorphisms do not seem to play a significant role in the propensity of S. aureus to colonise human nasal epithelium. Furthermore, based on the genetic heterogeneity encountered among the S. aureus strains it is suggested that within the current study population, no single clonal lineage of S. aureus has increased capability to colonise the human nasal epithelium.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>9126823</pmid><doi>10.1099/00222615-46-3-222</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of medical microbiology, 1997-03, Vol.46 (3), p.222-232 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adult Aged Bacteriology Bacteriophage Typing Biological and medical sciences Carrier State - microbiology Coagulase - genetics DNA, Bacterial - analysis Female Fundamental and applied biological sciences. Psychology Genetic Variation Genotype Humans Longitudinal Studies Male Microbiology Middle Aged Nasal Mucosa - microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Phenotype Polymerase Chain Reaction Polymorphism, Genetic Random Amplified Polymorphic DNA Technique Staphylococcal Infections - microbiology Staphylococcal Protein A - genetics Staphylococcus aureus - classification Staphylococcus aureus - genetics Staphylococcus aureus - pathogenicity Virulence |
| title | Coagulase and protein A polymorphisms do not contribute to persistence of nasal colonisation by Staphylococcus aureus |
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