Molecular cloning of the leub genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis
A gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pU...
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| Veröffentlicht in: | Biochemistry and molecular biology international 1997-04, Vol.41 (4), p.657-663 |
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| container_end_page | 663 |
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| container_issue | 4 |
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| container_title | Biochemistry and molecular biology international |
| container_volume | 41 |
| creator | Han, Mi Young Son, Mi Young Lee, Seung‐Hwan Kim, Jin Koo Huh, Jae Suk Kim, Jeong Hyun Choe, In Seong Chung, Tai Wha Choe, Yong‐Kyung |
| description | A gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pUC 19 digested with BamHI. Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1,011 bp encoding isopropytmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp. |
| doi_str_mv | 10.1080/15216549700201701 |
| format | Article |
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Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1,011 bp encoding isopropytmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp.</description><identifier>ISSN: 1521-6543</identifier><identifier>ISSN: 1039-9712</identifier><identifier>EISSN: 1521-6551</identifier><identifier>DOI: 10.1080/15216549700201701</identifier><identifier>PMID: 9111927</identifier><language>eng</language><publisher>UK: Informa Healthcare</publisher><subject>3-Isopropylmalate Dehydrogenase ; Alcohol Oxidoreductases - genetics ; Amino Acid Sequence ; Base Sequence ; BCG ; Cloning, Molecular ; Escherichia coli - genetics ; Genes, Bacterial - genetics ; Genetic Complementation Test ; Humans ; isopropylmalate dehydrogenase gene ; Korea ; leuB ; Leucine - biosynthesis ; M. tuberculosis ; Molecular Sequence Data ; Mycobacterium bovis - genetics ; Mycobacterium tuberculosis - genetics ; Restriction Mapping ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Tuberculosis - microbiology</subject><ispartof>Biochemistry and molecular biology international, 1997-04, Vol.41 (4), p.657-663</ispartof><rights>Copyright © 1997 International Union of Biochemistry and Molecular Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1080%2F15216549700201701$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1080%2F15216549700201701$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9111927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Mi Young</creatorcontrib><creatorcontrib>Son, Mi Young</creatorcontrib><creatorcontrib>Lee, Seung‐Hwan</creatorcontrib><creatorcontrib>Kim, Jin Koo</creatorcontrib><creatorcontrib>Huh, Jae Suk</creatorcontrib><creatorcontrib>Kim, Jeong Hyun</creatorcontrib><creatorcontrib>Choe, In Seong</creatorcontrib><creatorcontrib>Chung, Tai Wha</creatorcontrib><creatorcontrib>Choe, Yong‐Kyung</creatorcontrib><title>Molecular cloning of the leub genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis</title><title>Biochemistry and molecular biology international</title><addtitle>Biochem Mol Biol Int</addtitle><description>A gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pUC 19 digested with BamHI. Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1,011 bp encoding isopropytmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp.</description><subject>3-Isopropylmalate Dehydrogenase</subject><subject>Alcohol Oxidoreductases - genetics</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>BCG</subject><subject>Cloning, Molecular</subject><subject>Escherichia coli - genetics</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetic Complementation Test</subject><subject>Humans</subject><subject>isopropylmalate dehydrogenase gene</subject><subject>Korea</subject><subject>leuB</subject><subject>Leucine - biosynthesis</subject><subject>M. tuberculosis</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium bovis - genetics</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Restriction Mapping</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Tuberculosis - microbiology</subject><issn>1521-6543</issn><issn>1039-9712</issn><issn>1521-6551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDFPwzAQhS0EKlD4AQxIntgCPieOE4mFVlCQWrGU2YrtSwlK4mInoP57UrXqAAO33Onee9_wCLkCdgssY3cgOKQiySVjnIFkcETOtr8oFQKOD3cSn5LzED7YMJLlIzLKASDn8oyohavR9HXhqaldW7Ur6kravSOtsdd0hS0GWnrX0MXGOF2YDn3VN1S7ryrQyXRGi9b-0rpeox-YLlThgpyURR3wcr_H5O3pcTl9juavs5fpwzwyMQMRyVKI1HIDnIOMIbFcAzNoizIGmyAWUoJJU61lafOcWVuajEMep0ZYqUUWj8nNjrv27rPH0KmmCgbrumjR9UHJLE8zBjAYYWc03oXgsVRrXzWF3yhgaluq-lPqkLnew3vdoD0k9i0O-v1O_65q3PwPVMvJYiIFcOCCifgHGeKCoQ</recordid><startdate>199704</startdate><enddate>199704</enddate><creator>Han, Mi Young</creator><creator>Son, Mi Young</creator><creator>Lee, Seung‐Hwan</creator><creator>Kim, Jin Koo</creator><creator>Huh, Jae Suk</creator><creator>Kim, Jeong Hyun</creator><creator>Choe, In Seong</creator><creator>Chung, Tai Wha</creator><creator>Choe, Yong‐Kyung</creator><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199704</creationdate><title>Molecular cloning of the leub genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis</title><author>Han, Mi Young ; Son, Mi Young ; Lee, Seung‐Hwan ; Kim, Jin Koo ; Huh, Jae Suk ; Kim, Jeong Hyun ; Choe, In Seong ; Chung, Tai Wha ; Choe, Yong‐Kyung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3015-7f556d2c12217314d2b10cedaf31d4eea771c66bb7fd990ddfc821936c5d7b583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>3-Isopropylmalate Dehydrogenase</topic><topic>Alcohol Oxidoreductases - genetics</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>BCG</topic><topic>Cloning, Molecular</topic><topic>Escherichia coli - genetics</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetic Complementation Test</topic><topic>Humans</topic><topic>isopropylmalate dehydrogenase gene</topic><topic>Korea</topic><topic>leuB</topic><topic>Leucine - biosynthesis</topic><topic>M. tuberculosis</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium bovis - genetics</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Restriction Mapping</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Tuberculosis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Mi Young</creatorcontrib><creatorcontrib>Son, Mi Young</creatorcontrib><creatorcontrib>Lee, Seung‐Hwan</creatorcontrib><creatorcontrib>Kim, Jin Koo</creatorcontrib><creatorcontrib>Huh, Jae Suk</creatorcontrib><creatorcontrib>Kim, Jeong Hyun</creatorcontrib><creatorcontrib>Choe, In Seong</creatorcontrib><creatorcontrib>Chung, Tai Wha</creatorcontrib><creatorcontrib>Choe, Yong‐Kyung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry and molecular biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Mi Young</au><au>Son, Mi Young</au><au>Lee, Seung‐Hwan</au><au>Kim, Jin Koo</au><au>Huh, Jae Suk</au><au>Kim, Jeong Hyun</au><au>Choe, In Seong</au><au>Chung, Tai Wha</au><au>Choe, Yong‐Kyung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of the leub genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis</atitle><jtitle>Biochemistry and molecular biology international</jtitle><addtitle>Biochem Mol Biol Int</addtitle><date>1997-04</date><risdate>1997</risdate><volume>41</volume><issue>4</issue><spage>657</spage><epage>663</epage><pages>657-663</pages><issn>1521-6543</issn><issn>1039-9712</issn><eissn>1521-6551</eissn><abstract>A gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pUC 19 digested with BamHI. Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1,011 bp encoding isopropytmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp.</abstract><cop>UK</cop><pub>Informa Healthcare</pub><pmid>9111927</pmid><doi>10.1080/15216549700201701</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemistry and molecular biology international, 1997-04, Vol.41 (4), p.657-663 |
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| subjects | 3-Isopropylmalate Dehydrogenase Alcohol Oxidoreductases - genetics Amino Acid Sequence Base Sequence BCG Cloning, Molecular Escherichia coli - genetics Genes, Bacterial - genetics Genetic Complementation Test Humans isopropylmalate dehydrogenase gene Korea leuB Leucine - biosynthesis M. tuberculosis Molecular Sequence Data Mycobacterium bovis - genetics Mycobacterium tuberculosis - genetics Restriction Mapping Sequence Analysis, DNA Sequence Homology, Nucleic Acid Tuberculosis - microbiology |
| title | Molecular cloning of the leub genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis |
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