A qualitative and quantitative cytochemical assay of dihydrofolate reductase in erythroid cells
The distribution and intensity of dihydrofolate reductase (DHFR) cytochemically demonstrable was studied in erythroid cells. Cells of normal human bone marrow, of human erythroleukaemia (M6), and cells of the Friend (MEL) clone 745A murine erythroleukaemia (also after differentiation with dimethylsu...
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| Veröffentlicht in: | Acta histochemica 1989, Vol.85 (1), p.51-58 |
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| container_title | Acta histochemica |
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| creator | Nano, Rosanna Gerzeli, Giuseppe Invernizzi, Rosangela Supino, Rosanna |
| description | The distribution and intensity of dihydrofolate reductase (DHFR) cytochemically demonstrable was studied in erythroid cells. Cells of normal human bone marrow, of human erythroleukaemia (M6), and cells of the
Friend (MEL) clone 745A murine erythroleukaemia (also after differentiation with dimethylsulphoxide, DMSO) were stained according to GERZELI and DE PICEIS POLVER (1969) technique; quantification of the reaction product was made using a
Vickers M86 microdensitometer. The enzyme activity progressively decreased during the normal differentiation of the erythropoietic series while persisted at high levels in erythroleukaemia cells. It can be suggested that in the 1st case, the cytochemical pattern of dihydrofolate reductase may be a useful added tool for studying the erythroid differentiation. In the 2nd case, the increased level of this enzyme may be related to an amplification of the gene of DHFR in the malignant transformation. |
| doi_str_mv | 10.1016/S0065-1281(89)80098-4 |
| format | Article |
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Vickers M86 microdensitometer. The enzyme activity progressively decreased during the normal differentiation of the erythropoietic series while persisted at high levels in erythroleukaemia cells. It can be suggested that in the 1st case, the cytochemical pattern of dihydrofolate reductase may be a useful added tool for studying the erythroid differentiation. In the 2nd case, the increased level of this enzyme may be related to an amplification of the gene of DHFR in the malignant transformation.</description><identifier>ISSN: 0065-1281</identifier><identifier>EISSN: 1618-0372</identifier><identifier>DOI: 10.1016/S0065-1281(89)80098-4</identifier><identifier>PMID: 2496572</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Animals ; Bone Marrow - enzymology ; Bone Marrow - pathology ; Bone Marrow Cells ; Cells, Cultured ; Histocytochemistry ; Humans ; Leukemia, Erythroblastic, Acute - enzymology ; Leukemia, Erythroblastic, Acute - pathology ; Leukemia, Experimental - enzymology ; Leukemia, Experimental - pathology ; Mice ; Tetrahydrofolate Dehydrogenase - metabolism</subject><ispartof>Acta histochemica, 1989, Vol.85 (1), p.51-58</ispartof><rights>1989 VEB Gustav Fischer Verlag Jena</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-b0a941eee587b1a449e0345a125e78b97c8136499df906e530807676b4e227ab3</citedby><cites>FETCH-LOGICAL-c360t-b0a941eee587b1a449e0345a125e78b97c8136499df906e530807676b4e227ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0065-1281(89)80098-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27906,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2496572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nano, Rosanna</creatorcontrib><creatorcontrib>Gerzeli, Giuseppe</creatorcontrib><creatorcontrib>Invernizzi, Rosangela</creatorcontrib><creatorcontrib>Supino, Rosanna</creatorcontrib><title>A qualitative and quantitative cytochemical assay of dihydrofolate reductase in erythroid cells</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>The distribution and intensity of dihydrofolate reductase (DHFR) cytochemically demonstrable was studied in erythroid cells. Cells of normal human bone marrow, of human erythroleukaemia (M6), and cells of the
Friend (MEL) clone 745A murine erythroleukaemia (also after differentiation with dimethylsulphoxide, DMSO) were stained according to GERZELI and DE PICEIS POLVER (1969) technique; quantification of the reaction product was made using a
Vickers M86 microdensitometer. The enzyme activity progressively decreased during the normal differentiation of the erythropoietic series while persisted at high levels in erythroleukaemia cells. It can be suggested that in the 1st case, the cytochemical pattern of dihydrofolate reductase may be a useful added tool for studying the erythroid differentiation. In the 2nd case, the increased level of this enzyme may be related to an amplification of the gene of DHFR in the malignant transformation.</description><subject>Animals</subject><subject>Bone Marrow - enzymology</subject><subject>Bone Marrow - pathology</subject><subject>Bone Marrow Cells</subject><subject>Cells, Cultured</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Leukemia, Erythroblastic, Acute - enzymology</subject><subject>Leukemia, Erythroblastic, Acute - pathology</subject><subject>Leukemia, Experimental - enzymology</subject><subject>Leukemia, Experimental - pathology</subject><subject>Mice</subject><subject>Tetrahydrofolate Dehydrogenase - metabolism</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v2zAMhoVhQ5pm-wkBdCrWg1dJlvVxGoqiXQcU6GHbWZAlGtHgWIkkB_C_r_OxXnciSL7kSz4IrSn5RgkVd78IEU1FmaJflb5VhGhV8Q9oSQVVFakl-4iW75IrdJ3zXzKLSM0WaMG4Fo1kS2Tu8X60fSi2hANgO_hjPpR_BTeV6DawDc722OZsJxw77MNm8il2sbcFcAI_umIz4DBgSFPZpBg8dtD3-TP61Nk-w5dLXKE_T4-_H56rl9cfPx_uXypXC1KqlljNKQA0SrbUcq6B1LyxlDUgVaulU7QWXGvfaSKgqYkiUkjRcmBM2rZeoZvz3l2K-xFyMduQjxfYAeKYjVRaMD0vWaHmLHQp5pygM7sUtjZNhhJzBGtOYM2RmlHanMAaPs-tLwZjuwX_PnUhOfe_n_swf3kIkEx2AQYHPiRwxfgY_uPwBpkaiMs</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Nano, Rosanna</creator><creator>Gerzeli, Giuseppe</creator><creator>Invernizzi, Rosangela</creator><creator>Supino, Rosanna</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>A qualitative and quantitative cytochemical assay of dihydrofolate reductase in erythroid cells</title><author>Nano, Rosanna ; Gerzeli, Giuseppe ; Invernizzi, Rosangela ; Supino, Rosanna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-b0a941eee587b1a449e0345a125e78b97c8136499df906e530807676b4e227ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Bone Marrow - enzymology</topic><topic>Bone Marrow - pathology</topic><topic>Bone Marrow Cells</topic><topic>Cells, Cultured</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Leukemia, Erythroblastic, Acute - enzymology</topic><topic>Leukemia, Erythroblastic, Acute - pathology</topic><topic>Leukemia, Experimental - enzymology</topic><topic>Leukemia, Experimental - pathology</topic><topic>Mice</topic><topic>Tetrahydrofolate Dehydrogenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nano, Rosanna</creatorcontrib><creatorcontrib>Gerzeli, Giuseppe</creatorcontrib><creatorcontrib>Invernizzi, Rosangela</creatorcontrib><creatorcontrib>Supino, Rosanna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nano, Rosanna</au><au>Gerzeli, Giuseppe</au><au>Invernizzi, Rosangela</au><au>Supino, Rosanna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A qualitative and quantitative cytochemical assay of dihydrofolate reductase in erythroid cells</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>1989</date><risdate>1989</risdate><volume>85</volume><issue>1</issue><spage>51</spage><epage>58</epage><pages>51-58</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>The distribution and intensity of dihydrofolate reductase (DHFR) cytochemically demonstrable was studied in erythroid cells. Cells of normal human bone marrow, of human erythroleukaemia (M6), and cells of the
Friend (MEL) clone 745A murine erythroleukaemia (also after differentiation with dimethylsulphoxide, DMSO) were stained according to GERZELI and DE PICEIS POLVER (1969) technique; quantification of the reaction product was made using a
Vickers M86 microdensitometer. The enzyme activity progressively decreased during the normal differentiation of the erythropoietic series while persisted at high levels in erythroleukaemia cells. It can be suggested that in the 1st case, the cytochemical pattern of dihydrofolate reductase may be a useful added tool for studying the erythroid differentiation. In the 2nd case, the increased level of this enzyme may be related to an amplification of the gene of DHFR in the malignant transformation.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>2496572</pmid><doi>10.1016/S0065-1281(89)80098-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Acta histochemica, 1989, Vol.85 (1), p.51-58 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bone Marrow - enzymology Bone Marrow - pathology Bone Marrow Cells Cells, Cultured Histocytochemistry Humans Leukemia, Erythroblastic, Acute - enzymology Leukemia, Erythroblastic, Acute - pathology Leukemia, Experimental - enzymology Leukemia, Experimental - pathology Mice Tetrahydrofolate Dehydrogenase - metabolism |
| title | A qualitative and quantitative cytochemical assay of dihydrofolate reductase in erythroid cells |
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