Identification of high affinity receptors for vasoactive intestinal peptide on human lymphocytes of B cell lineage
Specific, high affinity receptors for vasoactive intestinal peptide (VIP) have been identified on a human pre-B cell line, Nalm 6, and on a human plasma cell line, Dakiki. The single class of high affinity sites exhibited a KD of 12.6 +/- 2.9 nM for VIP in Nalm 6 cells and 9.1 +/- 2.7 nM in Dakiki p...
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| Veröffentlicht in: | The Journal of immunology (1950) 1989-05, Vol.142 (10), p.3533-3536 |
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| container_end_page | 3536 |
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| container_issue | 10 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 142 |
| creator | O'Dorisio, MS Shannon, BT Fleshman, DJ Campolito, LB |
| description | Specific, high affinity receptors for vasoactive intestinal peptide (VIP) have been identified on a human pre-B cell line, Nalm 6, and on a human plasma cell line, Dakiki. The single class of high affinity sites exhibited a KD of 12.6 +/- 2.9 nM for VIP in Nalm 6 cells and 9.1 +/- 2.7 nM in Dakiki plasma cells. The homologous peptides, peptide histidine methionine (PHM), growth hormone releasing factor (GHRF), and secretin were all less effective than VIP in competitively inhibiting binding of 125I-VIP to Nalm 6 and Dakiki plasma membranes. The putative receptor was characterized as a 47-kDa protein using covalent cross-linking techniques and VIP stimulated adenylate cyclase in pre-B cells. Human lymphocytes of B cell lineage thus appear to express functional VIP receptors homologous to the receptor identified in T lymphoblasts, brain, pituitary, and intestine. |
| doi_str_mv | 10.4049/jimmunol.142.10.3533 |
| format | Article |
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Human lymphocytes of B cell lineage thus appear to express functional VIP receptors homologous to the receptor identified in T lymphoblasts, brain, pituitary, and intestine.</description><subject>Adenylyl Cyclases - metabolism</subject><subject>B-Lymphocytes - enzymology</subject><subject>B-Lymphocytes - metabolism</subject><subject>Cell Line</subject><subject>Humans</subject><subject>Leukemia, B-Cell - enzymology</subject><subject>Leukemia, B-Cell - metabolism</subject><subject>Membrane Proteins - metabolism</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Plasma Cells - metabolism</subject><subject>Receptors, Gastrointestinal Hormone - analysis</subject><subject>Receptors, Gastrointestinal Hormone - physiology</subject><subject>Receptors, Vasoactive Intestinal Peptide</subject><subject>Stem Cells - enzymology</subject><subject>Stem Cells - metabolism</subject><subject>Vasoactive Intestinal Peptide - metabolism</subject><subject>Vasoactive Intestinal Peptide - physiology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAQhUVJSbZp_0ELOoVevJVkybaOzZK2gUAv7VnI8iirIEuOZGfZf1-Z3ZbcehqY-d5jeA-hj5RsOeHyy5MbxyVEv6WcbcuyFnX9Bm2oEKRqGtJcoA0hjFW0bdor9C7nJ0JIQxi_RJdMcEql3KB0P0CYnXVGzy4GHC3eu8c91ta64OYjTmBgmmPK2MaEX3SO2szuBbALM-TZBe3xVAg3AC76_TLqgP1xnPbRHAuxOt5iA95j7wLoR3iP3lrtM3w4z2v0-9vdr92P6uHn9_vd14fK8LabKylADoTTllMBmg6ma5hmlmnb1tJY2_RUE0pBmBqobAUhA-lZLyTpikHd19fo5uQ7pfi8lF_V6PL6iA4Ql6zaTpaUZPNfkApWt123gvwEmhRzTmDVlNyo01FRotZO1N9OVOlkXa6dFNmns__SjzD8E51LKPfPp_ua_MElUHnU3heaqsPh8NrqD8JhmaU</recordid><startdate>19890515</startdate><enddate>19890515</enddate><creator>O'Dorisio, MS</creator><creator>Shannon, BT</creator><creator>Fleshman, DJ</creator><creator>Campolito, LB</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19890515</creationdate><title>Identification of high affinity receptors for vasoactive intestinal peptide on human lymphocytes of B cell lineage</title><author>O'Dorisio, MS ; Shannon, BT ; Fleshman, DJ ; Campolito, LB</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-95e9d0417415ea1dc862a2f2af739cff6b1a011e5c3e197500d0b2b5908c473b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adenylyl Cyclases - metabolism</topic><topic>B-Lymphocytes - enzymology</topic><topic>B-Lymphocytes - metabolism</topic><topic>Cell Line</topic><topic>Humans</topic><topic>Leukemia, B-Cell - enzymology</topic><topic>Leukemia, B-Cell - metabolism</topic><topic>Membrane Proteins - metabolism</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Plasma Cells - metabolism</topic><topic>Receptors, Gastrointestinal Hormone - analysis</topic><topic>Receptors, Gastrointestinal Hormone - physiology</topic><topic>Receptors, Vasoactive Intestinal Peptide</topic><topic>Stem Cells - enzymology</topic><topic>Stem Cells - metabolism</topic><topic>Vasoactive Intestinal Peptide - metabolism</topic><topic>Vasoactive Intestinal Peptide - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Dorisio, MS</creatorcontrib><creatorcontrib>Shannon, BT</creatorcontrib><creatorcontrib>Fleshman, DJ</creatorcontrib><creatorcontrib>Campolito, LB</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Dorisio, MS</au><au>Shannon, BT</au><au>Fleshman, DJ</au><au>Campolito, LB</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of high affinity receptors for vasoactive intestinal peptide on human lymphocytes of B cell lineage</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1989-05-15</date><risdate>1989</risdate><volume>142</volume><issue>10</issue><spage>3533</spage><epage>3536</epage><pages>3533-3536</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Specific, high affinity receptors for vasoactive intestinal peptide (VIP) have been identified on a human pre-B cell line, Nalm 6, and on a human plasma cell line, Dakiki. 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| ispartof | The Journal of immunology (1950), 1989-05, Vol.142 (10), p.3533-3536 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adenylyl Cyclases - metabolism B-Lymphocytes - enzymology B-Lymphocytes - metabolism Cell Line Humans Leukemia, B-Cell - enzymology Leukemia, B-Cell - metabolism Membrane Proteins - metabolism Neoplasm Proteins - metabolism Plasma Cells - metabolism Receptors, Gastrointestinal Hormone - analysis Receptors, Gastrointestinal Hormone - physiology Receptors, Vasoactive Intestinal Peptide Stem Cells - enzymology Stem Cells - metabolism Vasoactive Intestinal Peptide - metabolism Vasoactive Intestinal Peptide - physiology |
| title | Identification of high affinity receptors for vasoactive intestinal peptide on human lymphocytes of B cell lineage |
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