Polymorphism of a 35–48 kDa Plasmodium falciparum merozoite surface antigen
Glycoproteins of the asexual blood stages of Plasmodium falciparum were labelled with radioactive glucosamine and analysed by two-dimensional electrophoresis. Four major glycoproteins were detected in all eight parasite isolates studied. Two of the glycoproteins, designated GP2 and GP4, were invaria...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1989-04, Vol.34 (1), p.79-86 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Fenton, Brian Clark, John T. Wilson, Christina F. McBride, Jana S. Walliker, David |
| description | Glycoproteins of the asexual blood stages of
Plasmodium falciparum were labelled with radioactive glucosamine and analysed by two-dimensional electrophoresis. Four major glycoproteins were detected in all eight parasite isolates studied. Two of the glycoproteins, designated GP2 and GP4, were invariant among the isolates, while the other two GP1 and GP3 were found to be polymorphic in both their biochemical and antigenic properties. By immunoblotting and immunoprecipitation with specific monoclonal antibodies, the two polymorphic glycoproteins were identified as surface antigens of merozoites. |
| doi_str_mv | 10.1016/0166-6851(89)90022-4 |
| format | Article |
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Plasmodium falciparum were labelled with radioactive glucosamine and analysed by two-dimensional electrophoresis. Four major glycoproteins were detected in all eight parasite isolates studied. Two of the glycoproteins, designated GP2 and GP4, were invariant among the isolates, while the other two GP1 and GP3 were found to be polymorphic in both their biochemical and antigenic properties. By immunoblotting and immunoprecipitation with specific monoclonal antibodies, the two polymorphic glycoproteins were identified as surface antigens of merozoites.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(89)90022-4</identifier><identifier>PMID: 2651919</identifier><identifier>CODEN: MBIPDP</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; Antigenic diversity ; Antigens, Protozoan - analysis ; Antigens, Surface - analysis ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Cloning, Molecular ; Electrophoresis, Gel, Two-Dimensional ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; Glycoproteins - analysis ; Human protozoal diseases ; Immunoblotting ; Infectious diseases ; Malaria ; Medical sciences ; Parasitic diseases ; Plasmodium falciparum - genetics ; Plasmodium falciparum - immunology ; Plasmodium falciparum, Two-dimensional electrophoresis, Monoclonal antibody ; Polymorphism, Genetic ; Precipitin Tests ; Protozoa ; Protozoal diseases ; Tropical medicine</subject><ispartof>Molecular and biochemical parasitology, 1989-04, Vol.34 (1), p.79-86</ispartof><rights>1989</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-7767d7bacf9a9679aa8511b0ee28081fc70987b7274316b6f6a3b7710958aa953</citedby><cites>FETCH-LOGICAL-c386t-7767d7bacf9a9679aa8511b0ee28081fc70987b7274316b6f6a3b7710958aa953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0166685189900224$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7160993$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2651919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fenton, Brian</creatorcontrib><creatorcontrib>Clark, John T.</creatorcontrib><creatorcontrib>Wilson, Christina F.</creatorcontrib><creatorcontrib>McBride, Jana S.</creatorcontrib><creatorcontrib>Walliker, David</creatorcontrib><title>Polymorphism of a 35–48 kDa Plasmodium falciparum merozoite surface antigen</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>Glycoproteins of the asexual blood stages of
Plasmodium falciparum were labelled with radioactive glucosamine and analysed by two-dimensional electrophoresis. Four major glycoproteins were detected in all eight parasite isolates studied. Two of the glycoproteins, designated GP2 and GP4, were invariant among the isolates, while the other two GP1 and GP3 were found to be polymorphic in both their biochemical and antigenic properties. By immunoblotting and immunoprecipitation with specific monoclonal antibodies, the two polymorphic glycoproteins were identified as surface antigens of merozoites.</description><subject>Animals</subject><subject>Antigenic diversity</subject><subject>Antigens, Protozoan - analysis</subject><subject>Antigens, Surface - analysis</subject><subject>Biochemistry. Physiology. Immunology. 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Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. 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Plasmodium falciparum were labelled with radioactive glucosamine and analysed by two-dimensional electrophoresis. Four major glycoproteins were detected in all eight parasite isolates studied. Two of the glycoproteins, designated GP2 and GP4, were invariant among the isolates, while the other two GP1 and GP3 were found to be polymorphic in both their biochemical and antigenic properties. By immunoblotting and immunoprecipitation with specific monoclonal antibodies, the two polymorphic glycoproteins were identified as surface antigens of merozoites.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>2651919</pmid><doi>10.1016/0166-6851(89)90022-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular and biochemical parasitology, 1989-04, Vol.34 (1), p.79-86 |
| issn | 0166-6851 1872-9428 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antigenic diversity Antigens, Protozoan - analysis Antigens, Surface - analysis Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Cloning, Molecular Electrophoresis, Gel, Two-Dimensional Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Glycoproteins Glycoproteins - analysis Human protozoal diseases Immunoblotting Infectious diseases Malaria Medical sciences Parasitic diseases Plasmodium falciparum - genetics Plasmodium falciparum - immunology Plasmodium falciparum, Two-dimensional electrophoresis, Monoclonal antibody Polymorphism, Genetic Precipitin Tests Protozoa Protozoal diseases Tropical medicine |
| title | Polymorphism of a 35–48 kDa Plasmodium falciparum merozoite surface antigen |
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