Differential determination of recombinant human interleukin-1α and its deamidated derivative by two sandwich enzyme immunoassays using monoclonal antibodies: Comparison with a polyclonal antibody-based competitive enzyme immunoassay

Using three different monoclonal antibodies (McAb no. 374, no. 964 and no. 1190) to human interleukin-1α (rHu-IL-1α), we have established two sandwich enzyme immunoassays (EIA) to differentiate rHu-IL-1α and its deamidated derivative (rHu-Asp 36-IL-1α) where the asparagine at position 36 (counting f...

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Veröffentlicht in:	Journal of immunological methods 1989-04, Vol.119 (1), p.75-82
Hauptverfasser:	Sunahara, N., Kawata, S., Kaibe, K., Furuta, R., Yamayoshi, M., Yamada, M., Kurooka, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - classification Aspartic Acid - metabolism Binding, Competitive Biological and medical sciences Deamidated human recombinant interleukin-1α Drug Stability Enzyme immunoassay Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immune Sera Immunoenzyme Techniques - standards Interleukin-1 - analogs & derivatives Interleukin-1 - analysis Interleukin-1 - pharmacokinetics Interleukin-1 - standards Interleukin-1α, human recombinant Kinetics Mice Mice, Inbred BALB C Mice, Inbred C3H Molecular immunology Monoclonal antibody Quality Control Rabbits Recombinant Proteins - analogs & derivatives Recombinant Proteins - analysis Recombinant Proteins - standards Techniques
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