Effect of Inclusion Body Contaminants on the Oxidative Renaturation of Hen Egg White Lysozyme

The effect of typical contaminants in inclusion body preparations such as DNA, ribosomal RNA, phospholipids, lipopolysaccharides, and other proteins on renaturation rate and yield of hen egg white lysozyme was investigated. Separate experiments were conducted in which known amounts of individual con...

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Veröffentlicht in:	Biotechnology progress 1997, Vol.13 (2), p.144-150
Hauptverfasser:	Maachupalli-Reddy, Jhansi, Kelley, Brian D., Clark, Eliana De Bernardez
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Biological and medical sciences Biotechnology DNA - chemistry Egg Proteins - chemistry Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Inclusion Bodies - chemistry Kinetics Lipopolysaccharides - chemistry Methods. Procedures. Technologies Miscellaneous Molecular and cellular biology Molecular genetics Muramidase - chemistry Oxidation-Reduction Phospholipids - chemistry Plasmids Protein Denaturation Protein Folding RNA, Ribosomal - chemistry Synthetic digonucleotides and genes. Sequencing Translation. Translation factors. Protein processing
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