16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis
The use of mitochondrial RNA as an indicator of apoptosis was investigated. Exposure of HA-1 fibroblastic cells to 10 μmol H 2O 2 per 10 7 cells induced nuclear fragmentation, cell shrinkage, and internucleosomal DNA fragmentation, all characteristics of apoptosis. RNA extracted from control and apo...
Gespeichert in:
| Veröffentlicht in: | Free radical biology & medicine 1997, Vol.22 (7), p.1295-1300 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1300 |
|---|---|
| container_issue | 7 |
| container_start_page | 1295 |
| container_title | Free radical biology & medicine |
| container_volume | 22 |
| creator | Crawford, Dana R Lauzon, Robert J Wang, Yanhong Mazurkiewicz, Joseph E Schools, Gary P Davies, Kelvin J.A |
| description | The use of mitochondrial RNA as an indicator of apoptosis was investigated. Exposure of HA-1 fibroblastic cells to 10
μmol H
2O
2 per 10
7 cells induced nuclear fragmentation, cell shrinkage, and internucleosomal DNA fragmentation, all characteristics of apoptosis. RNA extracted from control and apoptotic cultures, and analyzed by Northern blot hybridization, revealed a significant increase in the degradation of mitochondrial 16S ribosomal RNA (rRNA) that was associated with apoptosis. Conversely, minimal, if any, degradation of glyceraldehyde-3-phosphate dehydrogenase or actin mRNAs was observed. Similar results were obtained for HA-1 cells treated with the protein kinase inhibitor staurosporine, and for HT-2 T-lymphocytes induced to undergo apoptosis by interleukin-2 withdrawal. In addition, 16S rRNA degradation was an early event that was discernable well before chromatin condensation in hydrogen peroxide-treated HA-1 cells. These observations suggest that degradation of mitochondrial 16S ribosomal RNA is a new marker of mammalian cell apoptosis.
© 1997 Elsevier Science Inc. |
| doi_str_mv | 10.1016/S0891-5849(96)00544-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78945391</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0891584996005448</els_id><sourcerecordid>78945391</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-63a86dcd6ec679baf482769fd0496f6aa3e492aa114c6b950dd739b149dee64f3</originalsourceid><addsrcrecordid>eNqFkM1PAjEQxRujQUT_BJI9GT2stmy32zkZgh-QoCai56bbzkoNUGwXjf-9y0e4cppJ3nvzMj9CuozeMMrE7YRKYGkuOVyBuKY05zyVR6TNZJGlPAdxTNp7yyk5i_GLUsrzTLZICyhIRvM2GTIxSZ5d7c3UL2xwepa8udJHP19vL_3kHj-Dtrp2fpGMYtKP0Runa7TJr6unSX_pl7WPLp6Tk0rPIl7sZod8PD68D4bp-PVpNOiPU5MBq1ORaSmssQKNKKDUFZe9QkBlKQdRCa0z5NDTmjFuRAk5tbbIoGQcLKLgVdYhl9u7y-C_VxhrNXfR4GymF-hXURUSmh-BHTSyBlHesGqM-dZogo8xYKWWwc11-FOMqjVqtUGt1hwVCLVBrWST6-4KVuUc7T61Y9vod1sdGxw_DoOKxuHCoHUBTa2sdwca_gHFmI1j</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15965873</pqid></control><display><type>article</type><title>16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Crawford, Dana R ; Lauzon, Robert J ; Wang, Yanhong ; Mazurkiewicz, Joseph E ; Schools, Gary P ; Davies, Kelvin J.A</creator><creatorcontrib>Crawford, Dana R ; Lauzon, Robert J ; Wang, Yanhong ; Mazurkiewicz, Joseph E ; Schools, Gary P ; Davies, Kelvin J.A</creatorcontrib><description>The use of mitochondrial RNA as an indicator of apoptosis was investigated. Exposure of HA-1 fibroblastic cells to 10
μmol H
2O
2 per 10
7 cells induced nuclear fragmentation, cell shrinkage, and internucleosomal DNA fragmentation, all characteristics of apoptosis. RNA extracted from control and apoptotic cultures, and analyzed by Northern blot hybridization, revealed a significant increase in the degradation of mitochondrial 16S ribosomal RNA (rRNA) that was associated with apoptosis. Conversely, minimal, if any, degradation of glyceraldehyde-3-phosphate dehydrogenase or actin mRNAs was observed. Similar results were obtained for HA-1 cells treated with the protein kinase inhibitor staurosporine, and for HT-2 T-lymphocytes induced to undergo apoptosis by interleukin-2 withdrawal. In addition, 16S rRNA degradation was an early event that was discernable well before chromatin condensation in hydrogen peroxide-treated HA-1 cells. These observations suggest that degradation of mitochondrial 16S ribosomal RNA is a new marker of mammalian cell apoptosis.
© 1997 Elsevier Science Inc.</description><identifier>ISSN: 0891-5849</identifier><identifier>EISSN: 1873-4596</identifier><identifier>DOI: 10.1016/S0891-5849(96)00544-8</identifier><identifier>PMID: 9098105</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Apoptosis ; Biomarkers ; Cell Line ; Cell Size - drug effects ; CHO Cells ; Cricetinae ; DNA Fragmentation ; Enzyme Inhibitors - pharmacology ; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; IL-2 withdrawal ; Interleukin-2 - pharmacology ; Mice ; Mitochondria - metabolism ; Programmed cell death ; RNA - metabolism ; RNA, Mitochondrial ; RNA, Ribosomal, 16S - metabolism ; Staurosporine ; Staurosporine - pharmacology ; T-Lymphocytes</subject><ispartof>Free radical biology & medicine, 1997, Vol.22 (7), p.1295-1300</ispartof><rights>1996 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-63a86dcd6ec679baf482769fd0496f6aa3e492aa114c6b950dd739b149dee64f3</citedby><cites>FETCH-LOGICAL-c391t-63a86dcd6ec679baf482769fd0496f6aa3e492aa114c6b950dd739b149dee64f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0891-5849(96)00544-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9098105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Crawford, Dana R</creatorcontrib><creatorcontrib>Lauzon, Robert J</creatorcontrib><creatorcontrib>Wang, Yanhong</creatorcontrib><creatorcontrib>Mazurkiewicz, Joseph E</creatorcontrib><creatorcontrib>Schools, Gary P</creatorcontrib><creatorcontrib>Davies, Kelvin J.A</creatorcontrib><title>16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis</title><title>Free radical biology & medicine</title><addtitle>Free Radic Biol Med</addtitle><description>The use of mitochondrial RNA as an indicator of apoptosis was investigated. Exposure of HA-1 fibroblastic cells to 10
μmol H
2O
2 per 10
7 cells induced nuclear fragmentation, cell shrinkage, and internucleosomal DNA fragmentation, all characteristics of apoptosis. RNA extracted from control and apoptotic cultures, and analyzed by Northern blot hybridization, revealed a significant increase in the degradation of mitochondrial 16S ribosomal RNA (rRNA) that was associated with apoptosis. Conversely, minimal, if any, degradation of glyceraldehyde-3-phosphate dehydrogenase or actin mRNAs was observed. Similar results were obtained for HA-1 cells treated with the protein kinase inhibitor staurosporine, and for HT-2 T-lymphocytes induced to undergo apoptosis by interleukin-2 withdrawal. In addition, 16S rRNA degradation was an early event that was discernable well before chromatin condensation in hydrogen peroxide-treated HA-1 cells. These observations suggest that degradation of mitochondrial 16S ribosomal RNA is a new marker of mammalian cell apoptosis.
© 1997 Elsevier Science Inc.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomarkers</subject><subject>Cell Line</subject><subject>Cell Size - drug effects</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>DNA Fragmentation</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>IL-2 withdrawal</subject><subject>Interleukin-2 - pharmacology</subject><subject>Mice</subject><subject>Mitochondria - metabolism</subject><subject>Programmed cell death</subject><subject>RNA - metabolism</subject><subject>RNA, Mitochondrial</subject><subject>RNA, Ribosomal, 16S - metabolism</subject><subject>Staurosporine</subject><subject>Staurosporine - pharmacology</subject><subject>T-Lymphocytes</subject><issn>0891-5849</issn><issn>1873-4596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1PAjEQxRujQUT_BJI9GT2stmy32zkZgh-QoCai56bbzkoNUGwXjf-9y0e4cppJ3nvzMj9CuozeMMrE7YRKYGkuOVyBuKY05zyVR6TNZJGlPAdxTNp7yyk5i_GLUsrzTLZICyhIRvM2GTIxSZ5d7c3UL2xwepa8udJHP19vL_3kHj-Dtrp2fpGMYtKP0Runa7TJr6unSX_pl7WPLp6Tk0rPIl7sZod8PD68D4bp-PVpNOiPU5MBq1ORaSmssQKNKKDUFZe9QkBlKQdRCa0z5NDTmjFuRAk5tbbIoGQcLKLgVdYhl9u7y-C_VxhrNXfR4GymF-hXURUSmh-BHTSyBlHesGqM-dZogo8xYKWWwc11-FOMqjVqtUGt1hwVCLVBrWST6-4KVuUc7T61Y9vod1sdGxw_DoOKxuHCoHUBTa2sdwca_gHFmI1j</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Crawford, Dana R</creator><creator>Lauzon, Robert J</creator><creator>Wang, Yanhong</creator><creator>Mazurkiewicz, Joseph E</creator><creator>Schools, Gary P</creator><creator>Davies, Kelvin J.A</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis</title><author>Crawford, Dana R ; Lauzon, Robert J ; Wang, Yanhong ; Mazurkiewicz, Joseph E ; Schools, Gary P ; Davies, Kelvin J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-63a86dcd6ec679baf482769fd0496f6aa3e492aa114c6b950dd739b149dee64f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomarkers</topic><topic>Cell Line</topic><topic>Cell Size - drug effects</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>DNA Fragmentation</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>IL-2 withdrawal</topic><topic>Interleukin-2 - pharmacology</topic><topic>Mice</topic><topic>Mitochondria - metabolism</topic><topic>Programmed cell death</topic><topic>RNA - metabolism</topic><topic>RNA, Mitochondrial</topic><topic>RNA, Ribosomal, 16S - metabolism</topic><topic>Staurosporine</topic><topic>Staurosporine - pharmacology</topic><topic>T-Lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crawford, Dana R</creatorcontrib><creatorcontrib>Lauzon, Robert J</creatorcontrib><creatorcontrib>Wang, Yanhong</creatorcontrib><creatorcontrib>Mazurkiewicz, Joseph E</creatorcontrib><creatorcontrib>Schools, Gary P</creatorcontrib><creatorcontrib>Davies, Kelvin J.A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical biology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crawford, Dana R</au><au>Lauzon, Robert J</au><au>Wang, Yanhong</au><au>Mazurkiewicz, Joseph E</au><au>Schools, Gary P</au><au>Davies, Kelvin J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis</atitle><jtitle>Free radical biology & medicine</jtitle><addtitle>Free Radic Biol Med</addtitle><date>1997</date><risdate>1997</risdate><volume>22</volume><issue>7</issue><spage>1295</spage><epage>1300</epage><pages>1295-1300</pages><issn>0891-5849</issn><eissn>1873-4596</eissn><abstract>The use of mitochondrial RNA as an indicator of apoptosis was investigated. Exposure of HA-1 fibroblastic cells to 10
μmol H
2O
2 per 10
7 cells induced nuclear fragmentation, cell shrinkage, and internucleosomal DNA fragmentation, all characteristics of apoptosis. RNA extracted from control and apoptotic cultures, and analyzed by Northern blot hybridization, revealed a significant increase in the degradation of mitochondrial 16S ribosomal RNA (rRNA) that was associated with apoptosis. Conversely, minimal, if any, degradation of glyceraldehyde-3-phosphate dehydrogenase or actin mRNAs was observed. Similar results were obtained for HA-1 cells treated with the protein kinase inhibitor staurosporine, and for HT-2 T-lymphocytes induced to undergo apoptosis by interleukin-2 withdrawal. In addition, 16S rRNA degradation was an early event that was discernable well before chromatin condensation in hydrogen peroxide-treated HA-1 cells. These observations suggest that degradation of mitochondrial 16S ribosomal RNA is a new marker of mammalian cell apoptosis.
© 1997 Elsevier Science Inc.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9098105</pmid><doi>10.1016/S0891-5849(96)00544-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0891-5849 |
| ispartof | Free radical biology & medicine, 1997, Vol.22 (7), p.1295-1300 |
| issn | 0891-5849 1873-4596 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78945391 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Apoptosis Biomarkers Cell Line Cell Size - drug effects CHO Cells Cricetinae DNA Fragmentation Enzyme Inhibitors - pharmacology Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Hydrogen peroxide Hydrogen Peroxide - pharmacology IL-2 withdrawal Interleukin-2 - pharmacology Mice Mitochondria - metabolism Programmed cell death RNA - metabolism RNA, Mitochondrial RNA, Ribosomal, 16S - metabolism Staurosporine Staurosporine - pharmacology T-Lymphocytes |
| title | 16S Mitochondrial Ribosomal RNA Degradation Is Associated with Apoptosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T18%3A43%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=16S%20Mitochondrial%20Ribosomal%20RNA%20Degradation%20Is%20Associated%20with%20Apoptosis&rft.jtitle=Free%20radical%20biology%20&%20medicine&rft.au=Crawford,%20Dana%20R&rft.date=1997&rft.volume=22&rft.issue=7&rft.spage=1295&rft.epage=1300&rft.pages=1295-1300&rft.issn=0891-5849&rft.eissn=1873-4596&rft_id=info:doi/10.1016/S0891-5849(96)00544-8&rft_dat=%3Cproquest_cross%3E78945391%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15965873&rft_id=info:pmid/9098105&rft_els_id=S0891584996005448&rfr_iscdi=true |