Inhibition of [ 3H]γ-aminobutyric acid release by kainate receptor activation in rat hippocampal synaptosomes
We studied the modulation of γ-aminobutyric acid (GABA) release by activation of kainate receptor in rat whole hippocampal synaptosomes. Kainate (10–300 μM) inhibited [ 3H]GABA release in a concentration-dependent manner with an EC 50 of 25 μM. This effect of kainate (30 μM) was prevented by the ion...
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| Veröffentlicht in: | European journal of pharmacology 1997-04, Vol.323 (2), p.167-172 |
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| creator | Cunha, Rodrigo A Constantino, M.Dolores Ribeiro, J.Alexandre |
| description | We studied the modulation of γ-aminobutyric acid (GABA) release by activation of kainate receptor in rat whole hippocampal synaptosomes. Kainate (10–300 μM) inhibited [
3H]GABA release in a concentration-dependent manner with an EC
50 of 25 μM. This effect of kainate (30 μM) was prevented by the ionotropic non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and by the selective kainate receptor antagonist 5-nitro-6,7,8,9-tetrahydrobenzo(
g)indole-2,3-dione-3-oxime (NS-102, 10 μM), but not by the selective non-competitive AMPA receptor antagonist 1-(4-aminophenyl)-4-methyl-7,8-methylenedioxy-5
H-2,3-benzodiazepine (GYKI 52466, 100 μM). Other kainate receptor agonists, such as domoic acid (0.3–10 μM) and (2
S,4
R)-4-methylglutamic acid (MGA, 0.3–3 μM), also inhibited [
3H]GABA release in a concentration-dependent manner with EC
50 values of 4.0 μM and 0.90 μM, respectively, whereas α-amino-3-hydroxy-5-methyl-4-oxazolepropionate (AMPA, 10–100 μM) was devoid of effect. These inhibitory effects of both domoic acid (3 μM) and MGA (1 μM) were antagonized by CNQX (10 μM). These results indicate that GABA release can be modulated directly by presynaptic high-affinity kainate heteroreceptors. ©
1997 Elsevier Science B.V. All rights reserved. |
| doi_str_mv | 10.1016/S0014-2999(97)00043-5 |
| format | Article |
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3H]GABA release in a concentration-dependent manner with an EC
50 of 25 μM. This effect of kainate (30 μM) was prevented by the ionotropic non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and by the selective kainate receptor antagonist 5-nitro-6,7,8,9-tetrahydrobenzo(
g)indole-2,3-dione-3-oxime (NS-102, 10 μM), but not by the selective non-competitive AMPA receptor antagonist 1-(4-aminophenyl)-4-methyl-7,8-methylenedioxy-5
H-2,3-benzodiazepine (GYKI 52466, 100 μM). Other kainate receptor agonists, such as domoic acid (0.3–10 μM) and (2
S,4
R)-4-methylglutamic acid (MGA, 0.3–3 μM), also inhibited [
3H]GABA release in a concentration-dependent manner with EC
50 values of 4.0 μM and 0.90 μM, respectively, whereas α-amino-3-hydroxy-5-methyl-4-oxazolepropionate (AMPA, 10–100 μM) was devoid of effect. These inhibitory effects of both domoic acid (3 μM) and MGA (1 μM) were antagonized by CNQX (10 μM). These results indicate that GABA release can be modulated directly by presynaptic high-affinity kainate heteroreceptors. ©
1997 Elsevier Science B.V. All rights reserved.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/S0014-2999(97)00043-5</identifier><identifier>PMID: 9128835</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Domoic acid ; Excitatory Amino Acid Agonists - pharmacology ; GABA (γ-aminobutyric acid) release ; GABA Antagonists - pharmacology ; Gabaergic and benzodiazepinic system ; gamma-Aminobutyric Acid - metabolism ; Glutamate ; Glutamates - pharmacology ; Hippocampus ; Hippocampus - metabolism ; Hippocampus - ultrastructure ; In Vitro Techniques ; Kainate ; Kainic Acid - analogs & derivatives ; Kainic Acid - pharmacology ; Male ; Medical sciences ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; Pharmacology. Drug treatments ; Rats ; Rats, Wistar ; Receptors, Kainic Acid - drug effects ; Synaptic Transmission - physiology ; Synaptosome ; Synaptosomes - metabolism</subject><ispartof>European journal of pharmacology, 1997-04, Vol.323 (2), p.167-172</ispartof><rights>1997 Elsevier Science B.V.</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-b6983aee235da9069a947e06484c86bed9776bfab022bee767d8b260b17c144d3</citedby><cites>FETCH-LOGICAL-c420t-b6983aee235da9069a947e06484c86bed9776bfab022bee767d8b260b17c144d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014299997000435$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2637620$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9128835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cunha, Rodrigo A</creatorcontrib><creatorcontrib>Constantino, M.Dolores</creatorcontrib><creatorcontrib>Ribeiro, J.Alexandre</creatorcontrib><title>Inhibition of [ 3H]γ-aminobutyric acid release by kainate receptor activation in rat hippocampal synaptosomes</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>We studied the modulation of γ-aminobutyric acid (GABA) release by activation of kainate receptor in rat whole hippocampal synaptosomes. Kainate (10–300 μM) inhibited [
3H]GABA release in a concentration-dependent manner with an EC
50 of 25 μM. This effect of kainate (30 μM) was prevented by the ionotropic non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and by the selective kainate receptor antagonist 5-nitro-6,7,8,9-tetrahydrobenzo(
g)indole-2,3-dione-3-oxime (NS-102, 10 μM), but not by the selective non-competitive AMPA receptor antagonist 1-(4-aminophenyl)-4-methyl-7,8-methylenedioxy-5
H-2,3-benzodiazepine (GYKI 52466, 100 μM). Other kainate receptor agonists, such as domoic acid (0.3–10 μM) and (2
S,4
R)-4-methylglutamic acid (MGA, 0.3–3 μM), also inhibited [
3H]GABA release in a concentration-dependent manner with EC
50 values of 4.0 μM and 0.90 μM, respectively, whereas α-amino-3-hydroxy-5-methyl-4-oxazolepropionate (AMPA, 10–100 μM) was devoid of effect. These inhibitory effects of both domoic acid (3 μM) and MGA (1 μM) were antagonized by CNQX (10 μM). These results indicate that GABA release can be modulated directly by presynaptic high-affinity kainate heteroreceptors. ©
1997 Elsevier Science B.V. All rights reserved.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Domoic acid</subject><subject>Excitatory Amino Acid Agonists - pharmacology</subject><subject>GABA (γ-aminobutyric acid) release</subject><subject>GABA Antagonists - pharmacology</subject><subject>Gabaergic and benzodiazepinic system</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Glutamate</subject><subject>Glutamates - pharmacology</subject><subject>Hippocampus</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - ultrastructure</subject><subject>In Vitro Techniques</subject><subject>Kainate</subject><subject>Kainic Acid - analogs & derivatives</subject><subject>Kainic Acid - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Kainic Acid - drug effects</subject><subject>Synaptic Transmission - physiology</subject><subject>Synaptosome</subject><subject>Synaptosomes - metabolism</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEQgIMo6-zqIyzkIOIeWpN0fjonkUXdhQUP6kkkVNI1bLQ7aZOehXku38NnsueHue6poOqrH-oj5JKzt5xx_e4rY1w2wlr7xporxphsG_WErHhnbMMMF0_J6oQ8J-e1_logZYU6I2eWi65r1Yqk23QffZxjTjSv6Q_a3vz897eBMabsN_O2xEAhxJ4WHBAqUr-lvyEmmHFJBZzmXBZgjg-wnxETLTDT-zhNOcA4wUDrNsGC1TxifUGerWGo-PIYL8j3Tx-_Xd80d18-315_uGuCFGxuvLZdC4iiVT1Ypi1YaZBp2cnQaY-9NUb7NXgmhEc02vSdF5p5bgKXsm8vyOvD3KnkPxussxtjDTgMkDBvqjOdlVJZ9ijItVSyVTtQHcBQcq0F124qcYSydZy5nRC3F-J233bWuL0Qp5a-y-OCjR-xP3UdDSz1V8c61ADDukAKsZ4woVujxW79-wOGy9ceIhZXQ8QUsI-Lhtn1OT5yyH_pK6k2</recordid><startdate>19970404</startdate><enddate>19970404</enddate><creator>Cunha, Rodrigo A</creator><creator>Constantino, M.Dolores</creator><creator>Ribeiro, J.Alexandre</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19970404</creationdate><title>Inhibition of [ 3H]γ-aminobutyric acid release by kainate receptor activation in rat hippocampal synaptosomes</title><author>Cunha, Rodrigo A ; Constantino, M.Dolores ; Ribeiro, J.Alexandre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-b6983aee235da9069a947e06484c86bed9776bfab022bee767d8b260b17c144d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Domoic acid</topic><topic>Excitatory Amino Acid Agonists - pharmacology</topic><topic>GABA (γ-aminobutyric acid) release</topic><topic>GABA Antagonists - pharmacology</topic><topic>Gabaergic and benzodiazepinic system</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Glutamate</topic><topic>Glutamates - pharmacology</topic><topic>Hippocampus</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - ultrastructure</topic><topic>In Vitro Techniques</topic><topic>Kainate</topic><topic>Kainic Acid - analogs & derivatives</topic><topic>Kainic Acid - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Kainic Acid - drug effects</topic><topic>Synaptic Transmission - physiology</topic><topic>Synaptosome</topic><topic>Synaptosomes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cunha, Rodrigo A</creatorcontrib><creatorcontrib>Constantino, M.Dolores</creatorcontrib><creatorcontrib>Ribeiro, J.Alexandre</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cunha, Rodrigo A</au><au>Constantino, M.Dolores</au><au>Ribeiro, J.Alexandre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of [ 3H]γ-aminobutyric acid release by kainate receptor activation in rat hippocampal synaptosomes</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1997-04-04</date><risdate>1997</risdate><volume>323</volume><issue>2</issue><spage>167</spage><epage>172</epage><pages>167-172</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>We studied the modulation of γ-aminobutyric acid (GABA) release by activation of kainate receptor in rat whole hippocampal synaptosomes. Kainate (10–300 μM) inhibited [
3H]GABA release in a concentration-dependent manner with an EC
50 of 25 μM. This effect of kainate (30 μM) was prevented by the ionotropic non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and by the selective kainate receptor antagonist 5-nitro-6,7,8,9-tetrahydrobenzo(
g)indole-2,3-dione-3-oxime (NS-102, 10 μM), but not by the selective non-competitive AMPA receptor antagonist 1-(4-aminophenyl)-4-methyl-7,8-methylenedioxy-5
H-2,3-benzodiazepine (GYKI 52466, 100 μM). Other kainate receptor agonists, such as domoic acid (0.3–10 μM) and (2
S,4
R)-4-methylglutamic acid (MGA, 0.3–3 μM), also inhibited [
3H]GABA release in a concentration-dependent manner with EC
50 values of 4.0 μM and 0.90 μM, respectively, whereas α-amino-3-hydroxy-5-methyl-4-oxazolepropionate (AMPA, 10–100 μM) was devoid of effect. These inhibitory effects of both domoic acid (3 μM) and MGA (1 μM) were antagonized by CNQX (10 μM). These results indicate that GABA release can be modulated directly by presynaptic high-affinity kainate heteroreceptors. ©
1997 Elsevier Science B.V. All rights reserved.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9128835</pmid><doi>10.1016/S0014-2999(97)00043-5</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | European journal of pharmacology, 1997-04, Vol.323 (2), p.167-172 |
| issn | 0014-2999 1879-0712 |
| language | eng |
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| subjects | Animals Biological and medical sciences Domoic acid Excitatory Amino Acid Agonists - pharmacology GABA (γ-aminobutyric acid) release GABA Antagonists - pharmacology Gabaergic and benzodiazepinic system gamma-Aminobutyric Acid - metabolism Glutamate Glutamates - pharmacology Hippocampus Hippocampus - metabolism Hippocampus - ultrastructure In Vitro Techniques Kainate Kainic Acid - analogs & derivatives Kainic Acid - pharmacology Male Medical sciences Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Pharmacology. Drug treatments Rats Rats, Wistar Receptors, Kainic Acid - drug effects Synaptic Transmission - physiology Synaptosome Synaptosomes - metabolism |
| title | Inhibition of [ 3H]γ-aminobutyric acid release by kainate receptor activation in rat hippocampal synaptosomes |
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