Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants
A survey for the presence of aster yellows‐related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested‐PCR assays. Using a phytoplasma 16Srl group‐specific primer pair followed by RFLP analysis of amplified products, we were able to detect and...
Gespeichert in:
| Veröffentlicht in: | Insect molecular biology 1997-05, Vol.6 (2), p.115-121 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 121 |
|---|---|
| container_issue | 2 |
| container_start_page | 115 |
| container_title | Insect molecular biology |
| container_volume | 6 |
| creator | Alma, A. Bosco, D. Danielli, A. Bertaccini, A. Vibio, M. Arzone, A. |
| description | A survey for the presence of aster yellows‐related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested‐PCR assays. Using a phytoplasma 16Srl group‐specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma‐free Vicia faba seedlings. Two of these broad beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows‐related phytoplasmas in S. titanus. |
| doi_str_mv | 10.1111/j.1365-2583.1997.tb00079.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78942880</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78942880</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4385-68f177b35f5898d1bff0715c301239f93f706869e03efb3af6b5b51a5b0fd3c3</originalsourceid><addsrcrecordid>eNqVkctu2zAQRYmiReqk_YQCRBddVSppiq8sAtRGYhtI20UNdElQEhnR1asihVp_Hwo2vA0ym1ncO2cGcwH4jFGKY307pJgwmiypICmWkqchRwhxmR7fgMVFegsWSLJlghGn78G194doEpLJK3AlkZSU0wVod6Vpg7Ou0MF1Lews7KspdH2tfaM9dC00T0_-K2ynpq881G0JdTnWwc_W34Xuq86VZvQwuKDb2Fe6ruFg9GBKGIGV0XWoJhiBbfAfwDura28-nvsN2D_c79fb5PHXZrf-_pgUGRE0YcJiznNCLRVSlDi3FnFMC4LwkkgrieWICSYNIsbmRFuW05xiTXNkS1KQG_DlhO2H7t9ofFCN84Wp4w2mG73iQmZLIdCLRkylECyj0Xh7MhZD5_1grOoH1-hhUhipORR1UPPn1fx5NYeizqGoYxz-dN4y5o0pL6PnFKJ-d9L_u9pMryCr3Y8VxjMgOQGcD-Z4Aejhr2KccKr-_Nyo7SbbklW2VxvyDAmMrMw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15988645</pqid></control><display><type>article</type><title>Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Alma, A. ; Bosco, D. ; Danielli, A. ; Bertaccini, A. ; Vibio, M. ; Arzone, A.</creator><creatorcontrib>Alma, A. ; Bosco, D. ; Danielli, A. ; Bertaccini, A. ; Vibio, M. ; Arzone, A.</creatorcontrib><description>A survey for the presence of aster yellows‐related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested‐PCR assays. Using a phytoplasma 16Srl group‐specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma‐free Vicia faba seedlings. Two of these broad beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows‐related phytoplasmas in S. titanus.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/j.1365-2583.1997.tb00079.x</identifier><identifier>PMID: 9099575</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; aster yellows ; Cicadellidae ; DNA, Bacterial - analysis ; Fabaceae ; Insecta - microbiology ; Keywords ; Nymph ; Ovum ; phytoplasma ; Plants, Medicinal ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Scaphoideus titanus ; Tenericutes - genetics ; Tenericutes - isolation & purification ; transovarial transmission ; Vicia faba</subject><ispartof>Insect molecular biology, 1997-05, Vol.6 (2), p.115-121</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4385-68f177b35f5898d1bff0715c301239f93f706869e03efb3af6b5b51a5b0fd3c3</citedby><cites>FETCH-LOGICAL-c4385-68f177b35f5898d1bff0715c301239f93f706869e03efb3af6b5b51a5b0fd3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2583.1997.tb00079.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2583.1997.tb00079.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9099575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alma, A.</creatorcontrib><creatorcontrib>Bosco, D.</creatorcontrib><creatorcontrib>Danielli, A.</creatorcontrib><creatorcontrib>Bertaccini, A.</creatorcontrib><creatorcontrib>Vibio, M.</creatorcontrib><creatorcontrib>Arzone, A.</creatorcontrib><title>Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>A survey for the presence of aster yellows‐related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested‐PCR assays. Using a phytoplasma 16Srl group‐specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma‐free Vicia faba seedlings. Two of these broad beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows‐related phytoplasmas in S. titanus.</description><subject>Animals</subject><subject>aster yellows</subject><subject>Cicadellidae</subject><subject>DNA, Bacterial - analysis</subject><subject>Fabaceae</subject><subject>Insecta - microbiology</subject><subject>Keywords</subject><subject>Nymph</subject><subject>Ovum</subject><subject>phytoplasma</subject><subject>Plants, Medicinal</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Scaphoideus titanus</subject><subject>Tenericutes - genetics</subject><subject>Tenericutes - isolation & purification</subject><subject>transovarial transmission</subject><subject>Vicia faba</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkctu2zAQRYmiReqk_YQCRBddVSppiq8sAtRGYhtI20UNdElQEhnR1asihVp_Hwo2vA0ym1ncO2cGcwH4jFGKY307pJgwmiypICmWkqchRwhxmR7fgMVFegsWSLJlghGn78G194doEpLJK3AlkZSU0wVod6Vpg7Ou0MF1Lews7KspdH2tfaM9dC00T0_-K2ynpq881G0JdTnWwc_W34Xuq86VZvQwuKDb2Fe6ruFg9GBKGIGV0XWoJhiBbfAfwDura28-nvsN2D_c79fb5PHXZrf-_pgUGRE0YcJiznNCLRVSlDi3FnFMC4LwkkgrieWICSYNIsbmRFuW05xiTXNkS1KQG_DlhO2H7t9ofFCN84Wp4w2mG73iQmZLIdCLRkylECyj0Xh7MhZD5_1grOoH1-hhUhipORR1UPPn1fx5NYeizqGoYxz-dN4y5o0pL6PnFKJ-d9L_u9pMryCr3Y8VxjMgOQGcD-Z4Aejhr2KccKr-_Nyo7SbbklW2VxvyDAmMrMw</recordid><startdate>199705</startdate><enddate>199705</enddate><creator>Alma, A.</creator><creator>Bosco, D.</creator><creator>Danielli, A.</creator><creator>Bertaccini, A.</creator><creator>Vibio, M.</creator><creator>Arzone, A.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>199705</creationdate><title>Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants</title><author>Alma, A. ; Bosco, D. ; Danielli, A. ; Bertaccini, A. ; Vibio, M. ; Arzone, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4385-68f177b35f5898d1bff0715c301239f93f706869e03efb3af6b5b51a5b0fd3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>aster yellows</topic><topic>Cicadellidae</topic><topic>DNA, Bacterial - analysis</topic><topic>Fabaceae</topic><topic>Insecta - microbiology</topic><topic>Keywords</topic><topic>Nymph</topic><topic>Ovum</topic><topic>phytoplasma</topic><topic>Plants, Medicinal</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Scaphoideus titanus</topic><topic>Tenericutes - genetics</topic><topic>Tenericutes - isolation & purification</topic><topic>transovarial transmission</topic><topic>Vicia faba</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alma, A.</creatorcontrib><creatorcontrib>Bosco, D.</creatorcontrib><creatorcontrib>Danielli, A.</creatorcontrib><creatorcontrib>Bertaccini, A.</creatorcontrib><creatorcontrib>Vibio, M.</creatorcontrib><creatorcontrib>Arzone, A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alma, A.</au><au>Bosco, D.</au><au>Danielli, A.</au><au>Bertaccini, A.</au><au>Vibio, M.</au><au>Arzone, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>1997-05</date><risdate>1997</risdate><volume>6</volume><issue>2</issue><spage>115</spage><epage>121</epage><pages>115-121</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>A survey for the presence of aster yellows‐related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested‐PCR assays. Using a phytoplasma 16Srl group‐specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma‐free Vicia faba seedlings. Two of these broad beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows‐related phytoplasmas in S. titanus.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9099575</pmid><doi>10.1111/j.1365-2583.1997.tb00079.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0962-1075 |
| ispartof | Insect molecular biology, 1997-05, Vol.6 (2), p.115-121 |
| issn | 0962-1075 1365-2583 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78942880 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals aster yellows Cicadellidae DNA, Bacterial - analysis Fabaceae Insecta - microbiology Keywords Nymph Ovum phytoplasma Plants, Medicinal Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Scaphoideus titanus Tenericutes - genetics Tenericutes - isolation & purification transovarial transmission Vicia faba |
| title | Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T07%3A12%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20phytoplasmas%20in%20eggs,%20nymphs%20and%20adults%20of%20Scaphoideus%20titanus%20Ball%20reared%20on%20healthy%20plants&rft.jtitle=Insect%20molecular%20biology&rft.au=Alma,%20A.&rft.date=1997-05&rft.volume=6&rft.issue=2&rft.spage=115&rft.epage=121&rft.pages=115-121&rft.issn=0962-1075&rft.eissn=1365-2583&rft_id=info:doi/10.1111/j.1365-2583.1997.tb00079.x&rft_dat=%3Cproquest_cross%3E78942880%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15988645&rft_id=info:pmid/9099575&rfr_iscdi=true |